{"title":"Investigation of protective effects of Harpagophytumprocumbens extract in an experimental rat colitis model","authors":"MUSTAFA ERKEK, DİLEK AKSIT","doi":"10.21521/mw.6818","DOIUrl":null,"url":null,"abstract":"The aim of this study was to investigate the possible effects of Harpagophytum procumbens (HP) extract on pro-inflammatory cytokines, oxidative stress, apoptosis and antioxidant mechanisms in a rat colitis model induced by acetic acid. 40 Wistar albino rats were divided into five equal groups: (1) Control, (2) HP, (3) Colitis, (4) Colitis + HP, (5) Colitis + Sulfasalazine. Malondialdehyde (MDA), total antioxidant capacity (TAS), superoxide dismutase (SOD), nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), ceruloplasmin and sialic acid levels were analyzed in blood samples. In addition colon tissues were evaluated histopathologically and immunohistochemically (B-cell lymphoma 2 (Bcl-2) and Bcl-2 associated X protein (Bax)). A significant decrease in serum MDA, NO, TNF-α, IL-6, sialic acid levels, and an increase in serum SOD, TAS, ceruplasmin levels were observed in Colitis + HP group compared to Colitis group. On the other hand, HP administration moderately reduced histopathological damage. It was detected immunohistochemically (Bcl-2, Bax) that apoptosis levels increased in the Colitis group and decreased with HP application. HP administration ameliorated the biochemical and pathological changes caused by colitis in rats. According to the results obtained in this study; HP might have a protective effect by suppressing of proinflammatory cytokines, apoptosis and reduced oxidative stress in colitis.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21521/mw.6818","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
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Abstract
The aim of this study was to investigate the possible effects of Harpagophytum procumbens (HP) extract on pro-inflammatory cytokines, oxidative stress, apoptosis and antioxidant mechanisms in a rat colitis model induced by acetic acid. 40 Wistar albino rats were divided into five equal groups: (1) Control, (2) HP, (3) Colitis, (4) Colitis + HP, (5) Colitis + Sulfasalazine. Malondialdehyde (MDA), total antioxidant capacity (TAS), superoxide dismutase (SOD), nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), ceruloplasmin and sialic acid levels were analyzed in blood samples. In addition colon tissues were evaluated histopathologically and immunohistochemically (B-cell lymphoma 2 (Bcl-2) and Bcl-2 associated X protein (Bax)). A significant decrease in serum MDA, NO, TNF-α, IL-6, sialic acid levels, and an increase in serum SOD, TAS, ceruplasmin levels were observed in Colitis + HP group compared to Colitis group. On the other hand, HP administration moderately reduced histopathological damage. It was detected immunohistochemically (Bcl-2, Bax) that apoptosis levels increased in the Colitis group and decreased with HP application. HP administration ameliorated the biochemical and pathological changes caused by colitis in rats. According to the results obtained in this study; HP might have a protective effect by suppressing of proinflammatory cytokines, apoptosis and reduced oxidative stress in colitis.
期刊介绍：
"Medycyna Weterynaryjna" publishes various types of articles which are grouped in the following editorial categories: reviews, original studies, scientific and professional problems, the history of veterinary medicine, posthumous memoirs, as well as chronicles that briefly relate scientific advances and developments in the veterinary profession and medicine. The most important are the first two categories, which are published with short summaries in English. Moreover, from 2001 the editors of "Medycyna Weterynaryjna", bearing in mind market demands, has also started publishing entire works in English. Since 2008 the periodical has appeared in an electronic version. The following are available in this version: summaries of studies published from 1999 to 2005, full versions of all the studies published in the years 2006-2011 (in pdf files), and full versions of the English studies published in the current year (pdf). Only summaries of the remaining studies from the current year are available. In accordance with the principles accepted by the editors, the full versions of these texts will not be made available until next year.
All articles are evaluated twice by leading Polish scientists and professionals before they are considered for publication. For years now "Medycyna Weterynaryjna" has maintained a high standard thanks to this system. The review articles are actually succinct monographs dealing with specific scientific and professional problems that are based on the most recent findings. Original works have a particular value, since they present research carried out in Polish and international scientific centers.
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