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erum interleukin content and lipid metabolism in cows with subclinical ketosis 亚临床酮症奶牛血清白介素含量与脂质代谢
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6820
DMYTRO KIBKAŁO, OLGA TYMOSZENKO, HALINA WIKULINA
This paper analyses the diagnostic utility of interleukins (1, 4, 6) and lipid metabolism products (cholesterol, triacylglycerols, lipoprotein fractions) in subclinical ketosis in cows. It was found that serum levels of proinflammatory (IL-1 and IL-6) and anti-inflammatory (IL-4) interleukins in cows with ketosis did not differ from those in clinically healthy animals. This therefore indicates the absence of inflammation in the subclinical form of ketosis. These data were also confirmed by normal α1- and α2-globulin levels. IL-6 is known to increase the synthesis of acute phase inflammatory proteins. There was no significant difference in the IL-4 content between the experimental and control groups of cows. In the subclinical form of ketosis in cows, abnormalities in lipid and lipoprotein metabolism were detected in the form of hypertriglyceridaemia and hyperlipoproteinaemia with very low-density lipoproteins, an increase in low-density lipoproteins, and a decrease in very high-density lipoproteins. This was verified by similar changes in β-lipoprotein levels and lipoprotein fractions and was specific to the first stage of lipomobilisation syndrome.
本文分析了白介素(1,4,6)和脂质代谢产物(胆固醇、甘油三酯、脂蛋白)在奶牛亚临床酮症中的诊断价值。发现酮症奶牛血清中促炎(IL-1、IL-6)和抗炎(IL-4)白介素水平与临床健康动物无显著差异。因此,这表明在亚临床形式的酮症中没有炎症。α1-和α2-球蛋白水平正常也证实了这些数据。已知IL-6可以增加急性期炎症蛋白的合成。试验组与对照组奶牛IL-4含量差异不显著。在奶牛的亚临床酮症中,脂质和脂蛋白代谢异常表现为高甘油三酯血症和高脂蛋白血症,伴极低密度脂蛋白,低密度脂蛋白增加,极高密度脂蛋白减少。β-脂蛋白水平和脂蛋白组分的类似变化证实了这一点,这是脂肪动员综合征第一阶段的特异性变化。
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引用次数: 0
Use of rapid immunochromatographic tests and PCR for the detection of vaccine viruses (CPV, CDV CAV) in faeces and conjunctival sac swabs of vaccinated dogs 应用快速免疫层析试验和PCR检测接种犬粪便和结膜囊拭子中的疫苗病毒(CPV、CDV、CAV)
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6836
ŁUKASZ ADASZEK, MARIA PISAREK, MARTA STANIEC, ŁUKASZ MAZUREK, ANNA KUCHARCZYK, MAŁGORZATA RUTKOWSKA-SZULCZYK, ŁUKASZ DENEKA
The aim of the study was to determine whether the rapid diagnostic CPV Ag (VetExpert), CDV Ag (VetExpert) and CDV/CAV Ag (VetExpert) tests are able to detect the presence of vaccine viruses in faeces and conjunctival swabs (CDV) collected from dogs vaccinated against distemper, parvovirus and Rubarth’s disease. The tested dogs were administered vaccines once, and then faeces and conjunctival sac swabs were collected from all animals on days: 2, 5, 7, 10, 14, 20, 25 after the vaccination for molecular testing and to perform rapid immunochromatographic tests for the detection of CPV, CDV and CAV antigens. The immunochromatographic tests were not able to detect the presence of any of the tested pathogens during the 25-day observation period in the vaccinated dogs, although CPV was detected in faeces by PCR up to 20 days after vaccination, and CDV and CAV up to 14 days after vaccination. Summing up the results of our own research, it should be stated that rapid immunochromatographic tests are not able to detect CPV, CDV and CAV vaccine viruses in the faeces of animals subjected to vaccination, and in the case of CDV also in the conjunctival swabs, but they are an excellent tool for detecting these pathogens in animals infected with field strains of these viruses and showing clinical symptoms of the diseases.
该研究的目的是确定快速诊断CPV Ag (VetExpert)、CDV Ag (VetExpert)和CDV/CAV Ag (VetExpert)检测是否能够检测从接种过犬瘟热、细小病毒和鲁氏病疫苗的狗收集的粪便和结膜拭子(CDV)中存在疫苗病毒。接种一次疫苗后,于接种后第2、5、7、10、14、20、25天采集所有动物的粪便和结膜囊拭子进行分子检测和快速免疫层析检测CPV、CDV和CAV抗原。免疫层析试验未能在接种犬的25天观察期内检测到任何被测病原体的存在,尽管在接种后20天通过PCR在粪便中检测到CPV,在接种后14天通过PCR在粪便中检测到CDV和CAV。总结我们自己的研究结果,需要说明的是,快速免疫层析技术不能在接种动物的粪便中检测到CPV、CDV和CAV疫苗病毒,在结膜拭子中也不能检测到CDV,但在感染这些病毒的野外菌株的动物中检测这些病原体并显示疾病的临床症状是一种很好的工具。
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引用次数: 0
Combating infectious animal diseases in accordance with the Regulation EU2016/429 -Animal Health Law 根据EU2016/429条例-动物卫生法,与传染性动物疾病作斗争
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6732
M. Welz, M. Bruczyńska, Renata Kondrat, Przemysław Łoś, Paulina Piersiak, K. Anusz
Comparing to the former regulations, the methods of combating infectious animal diseases established in the Animal Health Law has undergone significant changes in many aspects, including the categorization of diseases, and the need to apply the provisions of this regulation directly may raise doubts both as to their interpretation and to the conduct of the authorities responsible for this area. In particular, this applies to domestic activities and the scope of competences of poviat veterinarians. An additional aspect that hinders the transparency of the application of the Animal Health Law is the principle of delegation of powers to the European Commission to issue delegated and implementing acts that supplement, specify or amend them. Therefore, we are dealing with the need to efficiently navigate the area of many new legal acts, the overall operational knowledge of which becomes indispensable, especially for employees and bodies of the Veterinary Inspectionin Poland.
与以前的条例相比,《动物卫生法》规定的防治传染性动物疾病的方法在许多方面发生了重大变化,包括疾病的分类,直接适用该条例的规定的必要性可能会对其解释和负责这一领域的当局的行为产生怀疑。特别是,这适用于家庭活动和私人兽医的权限范围。阻碍《动物卫生法》实施透明度的另一个方面是将权力下放给欧洲委员会的原则,由其颁布补充、具体说明或修改《动物卫生法》的授权法令和执行法令。因此,我们正在处理有效地驾驭许多新法律行为领域的需要,其中的整体操作知识变得不可或缺,特别是对于波兰兽医检查的员工和机构。
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引用次数: 0
Occurrence of the selected parasites and viral infections in horses and donkeys in Turkey 土耳其马和驴中选定寄生虫和病毒感染的发生情况
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6743
E. Baydar, U. Aydoğdu, A. E. Utuk, F. Kaya, O. Tımurkan, Ufuk Erol, C. Babur
The present study aimed to investigate the prevalence of certain protozoa (B. caballi, T. equi, T. gondii, Neospora sp.) and viral agents (equine influenza, equine viral arteritis, equine herpesviruses) of equids in Balikesir and its surroundings, in Turkey. Plasma and serum samples were collected from 66 horses and 96 donkeys. Babesia caballi, T. equi and Neospora sp. antibodies were detected with c-ELISA, whereas T. gondii antibodies were revealed by the Sabin Feldman Dye test. Viral agents were detected by the PCR technique. The prevalence rates of the protozoa in horses were 12.12% for B. caballi, 34.84% for T. equi, 9.09% for T. gondii, and 10.6% for Neospora sp. The molecular prevalence of the viral agents amounted to 3.03% for equine influenza virus and 6.06% for equine herpesvirus 5. Equine viral arteritis virus and other herpesviruses (1, 2 and 4) were not detected in any of the samples. The rates of seropositivity in donkeys were 1.69% for B. caballi, 71.87% for T. equi, 90.62% for T. gondii, 23.95% for Neospora sp., 1.04% for equine influenza virus, 0% for equine viral arteritis virus, 3.12% for equine herpesvirus 5, and 0% for other herpesviruses. This study is the first to report the existence of anti-Neospora sp. antibodies in donkeys and the seroprevalence of T. gondii in horses and donkeys in Western Anatolia, Turkey.
本研究旨在调查土耳其Balikesir及其周边地区马科动物中某些原生动物(卡巴利贝氏杆菌、马弓形虫、刚地弓形虫、新孢子虫等)和病毒因子(马流感、马病毒性动脉炎、马疱疹病毒)的流行情况。采集了66匹马和96头驴的血浆和血清样本。c-ELISA法检测卡巴贝斯虫、马氏弓形虫和新孢子虫抗体,Sabin Feldman Dye法检测弓形虫抗体。采用PCR技术检测病毒因子。马体内原虫感染率分别为:卡巴利布氏杆菌12.12%、马弓形虫34.84%、弓形虫9.09%、新孢子虫10.6%;马流感病毒和马疱疹病毒5型的分子流行率分别为3.03%和6.06%。在所有样本中均未检测到马病毒性动脉炎病毒和其他疱疹病毒(1、2和4)。毛驴血清阳性率分别为:卡巴利布氏杆菌1.69%、马弓形虫71.87%、弓形虫90.62%、新孢子虫23.95%、马流感病毒1.04%、马病毒性动脉炎病毒0%、马5型疱疹病毒3.12%、其他疱疹病毒0%。本研究首次报道了土耳其西部安纳托利亚地区驴中存在抗新孢子虫抗体,以及马和驴中弓形虫的血清阳性率。
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引用次数: 0
Fish as a source of foodborne bacteria 鱼类是食源性细菌的来源
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6739
Aleksandra Kobuszewska, B. Wysok
A significant part of the global population depends on fish for both survival and health. However, as industry and agriculture expand rapidly, more and more natural and artificial aquatic ecosystems become contaminated. It may not only have an impact on the health of fish, but also raise safety concerns regarding the human consumption of fish. Although fish and fish products are well known to have a great nutritional value, it is crucial to be aware of the risk associated with their consumption, as they are among the main sources of foodborne bacteria.
全球人口的很大一部分依靠鱼类生存和健康。然而,随着工农业的迅速发展,越来越多的自然和人工水生生态系统受到污染。这不仅会影响鱼类的健康,还会引起人们对食用鱼类的安全担忧。虽然众所周知鱼和鱼制品具有很高的营养价值,但至关重要的是要意识到与食用它们相关的风险,因为它们是食源性细菌的主要来源之一。
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引用次数: 1
Complications after surgical extraction of cheek teeth in horses 马颊齿手术拔除后的并发症
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6742
Marta Marcinek, P. Zielińska, Zdzisław Kiełbowicz
The aim of this article is to summarise the factors that influence the occurrence of complications after surgical extraction of teeth in horses. This is influenced not only by the choice of method, but also by the age of the horse, the position of the tooth, the experience of the person performing the procedure and the available diagnostics methods. It should be emphasised that the procedure is performed only when there is no possibility of further conservative treatment and an increasing clinical condition may be an important risk factor for complications. Further studies focusing on the topic are needed to fully assess the number of complications and the links between their occurrence and the relevant factor.
本文的目的是总结影响马匹拔牙术后并发症发生的因素。这不仅受到方法选择的影响,还受到马的年龄、牙齿的位置、执行程序的人的经验和可用的诊断方法的影响。需要强调的是,只有在没有进一步保守治疗的可能性和临床病情加重可能是并发症的重要危险因素时,才进行手术。需要进一步研究这一主题,以充分评估并发症的数量及其发生与相关因素之间的联系。
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引用次数: 0
Serum Mineral Levels and Haematobiochemical Parameters in Buffalo Calves with Allotriophagy (Pica Syndrome) 异食癖(异食癖)水牛犊牛血清矿物质水平及血液生化指标
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6753
A. Kochan, A. Simsek, E. Arıca
Pica is defined as a depraved or abnormal appetite characterized by licking and eating non-nutritive substances. The aim of this study was to report changes in the serum mineral levels and haematobiochemical parameters of buffalo calves with pica. The study included 30 buffalo calves exhibiting pica behaviour, such as licking stall walls or eating wool and non-food items (pica group), and 6 buffalo calves without the signs of pica behaviour (control group). The age of both groups ranged between 1 and 6 months. In the pica group, the haematological analysis revealed a decrease in red blood cells (P < 0.001) and red cell distribution width (P < 0.05) as well as an increase in the mean corpuscular volume and mean corpuscular haemoglobin (P < 0.001), whereas the biochemical parameters were characterized by an increase in aspartate aminotransferase (P < 0.01), alanine aminotransferase (P < 0.001) and lactate dehydrogenase (P < 0.05) levels and a decrease in creatinine and glucose (P < 0.01) levels. Furthermore, the pica group showed a significant decrease in serum manganese, iron, zinc, selenium (P < 0.001) and copper (P < 0.01) concentrations. In conclusion, it was determined that deficiencies in the levels of serum iron, copper, zinc, manganese and selenium play an important role in the aetiology of pica in buffalo calves.
异食癖被定义为以舔食非营养物质为特征的食欲减退或异常。本研究的目的是报道患有异食癖的水牛犊牛血清矿物质水平和血液生化参数的变化。该研究包括30头表现出异食癖行为的水牛,比如舔马厩墙壁或吃羊毛和非食物(异食癖组),以及6头没有异食癖行为迹象的水牛(对照组)。两组患儿年龄在1 ~ 6个月之间。异食癖组血液学的分析揭示了血红细胞减少(P < 0.001),红细胞分布宽度(P < 0.05)以及增加平均微粒体积和平均微粒血红蛋白(P < 0.001),而生化参数以天冬氨酸转氨酶增加(P < 0.01),丙氨酸转氨酶(P < 0.001)和乳酸脱氢酶(P < 0.05)水平,降低肌酐和葡萄糖(P < 0.01)水平。异食癖组血清锰、铁、锌、硒(P < 0.001)和铜(P < 0.01)浓度显著降低。综上所述,血清铁、铜、锌、锰和硒水平的缺乏在水牛犊牛异食癖的病因中起重要作用。
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引用次数: 0
Black Mulberry (Morus Nigra L.) juice has positive effects on spermatological parameters in male New Zealand White rabbits 黑桑(Morus Nigra L.)汁对雄性新西兰大白兔精子指标有积极影响
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6764
Özlem YILDIZ-GULAY, M. Gulay
The current study aimed to investigate the possible effects of black mulberry juice (BMJ) consumption on spermatologic parameters, oxidant/antioxidant activity, and seminal plasma testosterone concentrations of male rabbits. Twenty-four healthy adult New Zealand White rabbits were randomly divided into 4 groups (n = 6). By oral gavage, 3.0 mL of distilled water, 0.75 mL BMJ + 2.25 distilled water, 1.50 mL BMJ + 1.5 mL distilled water, and 3 mL BMJ per 1 kg body weight were given daily for 9 weeks to rabbits in the C, LD, MD, and HD groups, respectively. Ejaculates were taken at the beginning and end of the experiments. Progressive motility, sperm concentration, and intact sperm rates were significantly increased especially in MD and HD groups. A significant decrease in malondialdehyde concentrations was apparent due to BMJ consumption. Moreover, there was a marked increase in catalase and glutathione peroxidase levels in the seminal plasma of the MD and HD groups. The current results indicated that, although seminal plasma protein levels and seminal plasma glutathione levels were not altered, overall oral BMJ treatments were able to improve sperm quality and antioxidant states of the male rabbits.
本研究旨在探讨黑桑椹汁(BMJ)摄入对雄性家兔精子学参数、氧化/抗氧化活性和精浆睾酮浓度的可能影响。选取健康成年新西兰大白兔24只,随机分为4组(n = 6), C组、LD组、MD组、HD组每天分别灌胃蒸馏水3.0 mL、BMJ 0.75 mL + 2.25蒸馏水、BMJ 1.50 mL + 1.5 mL蒸馏水、BMJ 3 mL / 1 kg体重,连续9周。在实验的开始和结束时分别服用射精液。进行性运动、精子浓度和完整精子率显著增加,尤其是在MD和HD组。由于BMJ的消耗,丙二醛浓度明显下降。此外,MD组和HD组精浆中过氧化氢酶和谷胱甘肽过氧化物酶水平显著升高。目前的研究结果表明,尽管精浆蛋白和精浆谷胱甘肽水平没有改变,但总体上口服BMJ治疗能够改善雄性兔的精子质量和抗氧化状态。
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引用次数: 0
Feline leukemia as a still relevant problem in domestic and wild animals – the current state of knowledge 猫白血病作为一个在家养和野生动物中仍然相关的问题-目前的认识状况
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6770
Joanna Zdziennicka, Dominika Dobko, Beata Drzewiecka, Katarzyna Krać, Aleksandra Kozera, Dominika Nguyen Ngoc, J. Wessely-Szponder
The aim of this review is to present information about leukemia caused by feline leukemia virus (FeLV) infection in felids. Despite widespread vaccinations, leukemia is still one of the most common diseases in cats. FeLV infection occurs by direct contact between animals, especially through contact with saliva. Infection with this highly contagious virus is characterized by immunosuppression and non-specific signs, such as cytopenia or tumorigenesis. The virus is transmitted horizontally through close contact between cats and vertically from infected queens to their kittens. Persistently infected cats are the reservoir of the virus. Rapid ELISA tests and immunochromatography play a major role in diagnosis, but RT-PCR is the most reliable diagnostic method. There is no specific therapy against FeLV, so treatment is mainly symptomatic. Research on the use of antiviral drugs, i.e. tenofovir, raltegravir, and fozyvudine, is underway. Proper prophylaxis, including regular vaccination of animals, is important to minimize the risk and consequences of infection
本文综述了猫科动物白血病病毒(FeLV)感染引起的白血病的研究进展。尽管广泛接种疫苗,白血病仍然是猫最常见的疾病之一。FeLV感染发生在动物之间的直接接触,特别是通过唾液接触。感染这种高传染性病毒的特点是免疫抑制和非特异性体征,如细胞减少或肿瘤发生。该病毒通过猫之间的密切接触水平传播,并通过受感染的母猫向其小猫垂直传播。持续感染的猫是病毒的宿主。快速ELISA检测和免疫层析在诊断中起主要作用,但RT-PCR是最可靠的诊断方法。目前尚无针对FeLV的特异性治疗方法,因此治疗主要是对症治疗。目前正在研究使用抗病毒药物,即替诺福韦、雷替格拉韦和福西夫定。适当的预防措施,包括定期为动物接种疫苗,对于尽量减少感染的风险和后果非常重要
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引用次数: 0
Gastrointestinal ulceration in dogs with portosystemic shunt 门静脉系统分流犬的胃肠道溃疡
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-01-01 DOI: 10.21521/mw.6786
J. Frymus, P. Trębacz, M. Galanty
Portosystemic shunt (PSS) in dogs is a common abnormality in which blood from the intestines bypasses the liver flowing directly to the systemic circulation through an aberrant vessel. The lack of hepatic detoxication can lead to a fatal outcome. Treatment of choice is a surgical occlusion of the shunt. Gastrointestinal ulceration/ erosion (GUE) has been described as one of the numerous clinical consequences of PSS, As these lesions are a severe complication, and seem to be underestimated, this review presents the current knowledge about GUE in dogs with PSS. Although the pathogenesis of these lesions is not clear and experimental data are scarce, we conclude that proton pump inhibitors should be administered pre- and postoperatively to dogs with PSS to reduce the incidence of GUE and thus increase the recovery rate.
狗的门静脉系统分流(PSS)是一种常见的异常,即来自肠道的血液绕过肝脏,通过异常血管直接流入体循环。缺乏肝脏排毒会导致致命的后果。治疗的选择是手术阻断分流。胃肠道溃疡/糜烂(GUE)已被描述为PSS的众多临床后果之一,由于这些病变是一种严重的并发症,似乎被低估了,本文综述了目前关于PSS犬GUE的知识。虽然这些病变的发病机制尚不清楚,实验数据也很少,但我们认为,PSS犬术前和术后应给予质子泵抑制剂,以减少GUE的发生率,从而提高恢复率。
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引用次数: 1
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Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
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