Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virus

IF 1.9 3区农林科学 Q2 AGRONOMY Phytopathologia Mediterranea Pub Date : 2023-09-15 DOI:10.36253/phyto-14463

Arnaud G. BLOUIN, Nathalie DUBUIS, Justine BRODARD, Laure APOTHÉLOZ-PERRET-GENTIL, Denise ALTENBACH, Olivier SCHUMPP

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Abstract

Tomato production is an important part of the Swiss vegetable production with most tomato crops grown in greenhouses. Tomato plants are vulnerable to diseases caused by viruses, which can have significant impacts on crop production. This study reports the first detection of tomato fruit botch virus (ToFBV, Blunervirus solani) in Switzerland, from a tomato production site at the southern part of the Ticino region. The symptoms observed indicated presence of a viral pathogen, but tests against the most common tomato viruses were negative. Immunocapture of double-stranded RNA and its subsequent sequencing on a Flongle flowcell (Oxford Nanopore Technologies) identified the presence of ToFBV and southern tomato virus. The genome of the Swiss ToFBV isolate was very similar to that available in GenBank. Datamining of the sequence read archives found the virus in two other countries, with a highly conserved genome. With this study, there are now 12 near-complete genomes of ToFBV available, and the virus is recorded from ten countries. This study underlines the importance of continuous monitoring and research on emerging viruses in tomato production.

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有症状、广泛、不明显:番茄果实斑病病毒新发现

番茄生产是瑞士蔬菜生产的重要组成部分，大多数番茄作物在温室中种植。番茄植株容易受到病毒引起的病害，这对作物生产有重大影响。本研究报道了瑞士首次在提契诺州南部的一个番茄生产基地检测到番茄果腐病毒(ToFBV, brunervirus solani)。观察到的症状表明病毒病原体的存在，但对最常见的番茄病毒的测试是阴性的。双链RNA的免疫捕获及其随后在Flongle流动细胞(Oxford Nanopore Technologies)上的测序确定了ToFBV和南方番茄病毒的存在。瑞士ToFBV分离物的基因组与GenBank中提供的基因组非常相似。对序列读取档案的数据挖掘发现，该病毒存在于另外两个国家，具有高度保守的基因组。通过这项研究，现在有12个几乎完整的ToFBV基因组，并且来自10个国家记录了该病毒。这项研究强调了对番茄生产中出现的病毒进行持续监测和研究的重要性。

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来源期刊

Phytopathologia Mediterranea 生物-植物科学

CiteScore

4.40

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world. The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.