Sensitivitas Pemeriksaan Covid-19: Insulated Isothermal PCR (iiPCR) Dan Reverse Transcription PCR (RT-PCR)

Lisa Yunita, Dian Rachma Wijayanti, Apriani Riyanti
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Abstract

Covid-19 (Corona Virus disease 2019) is a new type of disease caused by a virus from the Corona Virus group, namely SARS-CoV-2. Covid-19 may cause respiratory system disorders, ranging from mild symptoms such as flu to lung infections, such as pneumonia. Laboratory diagnoses for Covid-19 disease generally include hematological (complete blood examinations), and molecular or a combination of serology and molecular. PCR examination that can be carried out using the Insulated Isothermal PCR and Reverse Transcription PCR methods. This research is descriptive observational research. Data were collected at Grha Kedoya Hospital, West Jakarta, North Kedoya. The result showed a sensitivity of iiPCR score 82,6%, which is relatively lower than gold-standard RT-PCR. Our research suggested that RT-PCR is still an effective and sensitive method for Covid-19 examination.
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pemeriksaa对Covid-19的敏感性:绝缘等温PCR (iiPCR)和反转录PCR (RT-PCR)
Covid-19(冠状病毒病2019)是由冠状病毒组中的一种病毒即SARS-CoV-2引起的新型疾病。Covid-19可能导致呼吸系统疾病,从流感等轻微症状到肺炎等肺部感染。Covid-19疾病的实验室诊断通常包括血液学(全血检查)、分子检查或血清学和分子检查的结合。采用绝缘等温PCR和反转录PCR方法进行PCR检测。本研究为描述性观察性研究。数据在西雅加达、北Kedoya的Grha Kedoya医院收集。结果显示,iiPCR的灵敏度为82.6%,相对低于金标准RT-PCR。我们的研究表明,RT-PCR仍然是一种有效和敏感的检测新冠病毒的方法。
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