Pub Date : 2023-05-31DOI: 10.30651/jmlt.v6i1.18153
Putu Ayu Parwati, Anak Agung Lilyk Cahyani
Bacteriuria is the leading indicator of Urinary Tract Infection (UTI), which is detected through urine culture examination. Significant bacteriuria indicates the growth of microorganisms as much as ≥ 100,000 cfu/ml in urine culture. There are drawbacks to this examination that requires a screening test with a short time and lower cost. This study aimed to determine differences in the results of nitrite and leukocyte esterase examination using a urine dipstick with urine culture as a predictor of bacteriuria. The type of research used is analytic with an experimental approach. The sample used in this study was ten samples of urine. Based on the bivariate analysis results, the p-value was 0.541, which means that there was no difference in the results of leukocyte esterase and urine culture. This indicates the presence of leukocytes in the urine can be an indication of bacteriuria. The results for the nitrite examination obtained a p-value of 0.000, which means there is a difference between the results of the nitrite examination and the results of the urine culture. This shows that the presence of nitrites in the urine cannot indicate of bacteriuria. However the data on the sensitivity and specificity of dipstick examination are still low for leukocyte esterase and urine nitrite tests. However, urine dipstick examination to detect bacteriuria and UTI is still an option for faster screening. Keywords : leukocyte esterase, nitrites, urine culture
{"title":"Penilaian Hasil Dipstik Urine (Nitrit Dan Leukosit Esterase) Sebagai Prediktor Bakteriuria","authors":"Putu Ayu Parwati, Anak Agung Lilyk Cahyani","doi":"10.30651/jmlt.v6i1.18153","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.18153","url":null,"abstract":"Bacteriuria is the leading indicator of Urinary Tract Infection (UTI), which is detected through urine culture examination. Significant bacteriuria indicates the growth of microorganisms as much as ≥ 100,000 cfu/ml in urine culture. There are drawbacks to this examination that requires a screening test with a short time and lower cost. This study aimed to determine differences in the results of nitrite and leukocyte esterase examination using a urine dipstick with urine culture as a predictor of bacteriuria. The type of research used is analytic with an experimental approach. The sample used in this study was ten samples of urine. Based on the bivariate analysis results, the p-value was 0.541, which means that there was no difference in the results of leukocyte esterase and urine culture. This indicates the presence of leukocytes in the urine can be an indication of bacteriuria. The results for the nitrite examination obtained a p-value of 0.000, which means there is a difference between the results of the nitrite examination and the results of the urine culture. This shows that the presence of nitrites in the urine cannot indicate of bacteriuria. However the data on the sensitivity and specificity of dipstick examination are still low for leukocyte esterase and urine nitrite tests. However, urine dipstick examination to detect bacteriuria and UTI is still an option for faster screening. Keywords : leukocyte esterase, nitrites, urine culture","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"67 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80883336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.30651/jmlt.v6i1.17683
Nisa Julia Safarti, B. Santosa, Purwanto Adhipireno
Chronic diabetes mellitus is carried on by a rise in blood glucose levels as a result of decreased insulin sensitivity to manage blood sugar levels. The purpose of this study was to analyze participants with controlled and uncontrolled type 2 diabetes in relation of blood glucose, insulin, Homa-IR, and SCUBE2 protein levels. This study has a cross-sectional design and is descriptive analytic research. The sample for this study was stored biological fluid which was collected by total sampling from the Roemani Muhammadiyah Hospital Semarang Laboratory Installation in September 2022 to October 2022. The T-Independent test and the Mann-Whitney U-test used to the data analysis. The results of the T-Independent test results suggest that there was no significant variance between the insulin levels in DMT2 patients who were in control and those who unable (p=0.820, p>0.05). According to the results of the Mann-Whitney U-test analysis, there was no significant difference in the levels of scube2 protein between controlled and uncontrolled T2DM, and there was a significant difference in the glucose levels and Homa-IR values between controlled and uncontrolled T2DM, including both.but there was a significant effect of differences in glucose levels and Homa-IR values. The conclusion is that there are differences in glucose levels and Homa-IR values in controlled and uncontrolled DMT2 and there is no difference in insulin and scube 2 protein levels in controlled and uncontrolled DMT2. Keywords: Glucose, Insulin, Homa-IR, Protein Scube2
慢性糖尿病是由于控制血糖水平的胰岛素敏感性降低而导致血糖水平升高。本研究的目的是分析控制型和非控制型2型糖尿病参与者的血糖、胰岛素、Homa-IR和SCUBE2蛋白水平的关系。本研究采用横断面设计，为描述性分析研究。本研究的样本是2022年9月至2022年10月在Roemani Muhammadiyah医院三宝郎实验室装置通过总采样收集的储存生物液。数据分析采用t独立检验和Mann-Whitney u检验。t独立检验结果显示，控制DMT2患者与不能控制DMT2患者胰岛素水平无显著差异(p=0.820, p < 0.05)。根据Mann-Whitney u检验分析结果，T2DM控制组与未控制组之间scube2蛋白水平无显著差异，血糖水平和Homa-IR值在控制组与未控制组之间均有显著差异。但血糖水平和Homa-IR值的差异有显著影响。结论:DMT2控制与不控制组血糖水平和Homa-IR值存在差异，而DMT2控制与不控制组胰岛素和scube 2蛋白水平无差异。关键词:葡萄糖，胰岛素，Homa-IR，蛋白Scube2
{"title":"Perbedaan Kadar Glukosa, Kadar Insulin, Homa IR Dan Protein Scube2 Pada Penderita DM Tipe 2 Terkontrol Dan Tidak Terkontrol","authors":"Nisa Julia Safarti, B. Santosa, Purwanto Adhipireno","doi":"10.30651/jmlt.v6i1.17683","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.17683","url":null,"abstract":"Chronic diabetes mellitus is carried on by a rise in blood glucose levels as a result of decreased insulin sensitivity to manage blood sugar levels. The purpose of this study was to analyze participants with controlled and uncontrolled type 2 diabetes in relation of blood glucose, insulin, Homa-IR, and SCUBE2 protein levels. This study has a cross-sectional design and is descriptive analytic research. The sample for this study was stored biological fluid which was collected by total sampling from the Roemani Muhammadiyah Hospital Semarang Laboratory Installation in September 2022 to October 2022. The T-Independent test and the Mann-Whitney U-test used to the data analysis. The results of the T-Independent test results suggest that there was no significant variance between the insulin levels in DMT2 patients who were in control and those who unable (p=0.820, p>0.05). According to the results of the Mann-Whitney U-test analysis, there was no significant difference in the levels of scube2 protein between controlled and uncontrolled T2DM, and there was a significant difference in the glucose levels and Homa-IR values between controlled and uncontrolled T2DM, including both.but there was a significant effect of differences in glucose levels and Homa-IR values. The conclusion is that there are differences in glucose levels and Homa-IR values in controlled and uncontrolled DMT2 and there is no difference in insulin and scube 2 protein levels in controlled and uncontrolled DMT2. Keywords: Glucose, Insulin, Homa-IR, Protein Scube2","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79791630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.30651/jmlt.v6i1.15887
R. Yuan
The corona virus outbreak first occurred at the end of 2019 in Wuhan, China. This virus then spread rapidly throughout the world, including Indonesia. So, countries in the world take steps to lock down. In Indonesia, large-scale social restrictions have been implemented in big cities with a high prevalence of the spread of the virus. Efforts to control the virus include limiting mobility, wearing masks, frequently washing hands or using hand sanitizers. Hand sanitizers are products made from ethanol which are increasingly limited and expensive during the Covid-19 pandemic. The purpose of this study is to make bioethanol with a variation of the fermentation time made from molasses, so as to produce ethanol content above 70%. This research is an experimental research. Production of bioethanol based on molasses, aquades, NPK, urea, and yeast culture of Saccharomycess cerevisae with time variations of 4, 5, and 6 days and distilled at a temperature of 500C. Testing the ethanol content using gas chromatography mass spectrometry. The results of this study, the fourth day of fermentation produces ethanol content of 50.23±0.42% with an average volume of 490 mL; day 5 produced ethanol content of 62.92±0.38% with an average volume of 385 mL; and the 6th day yielded ethanol content of 72.11±0.46% with an average volume of 243 mL. So, the 6th day of fermentation is the best fermentation to produce bioethanol as a raw material for hand sanitizer with ethanol content above 70%.
{"title":"Pengaruh Waktu Fermentasi Pada Produksi Bioethanol Dari Molase","authors":"R. Yuan","doi":"10.30651/jmlt.v6i1.15887","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.15887","url":null,"abstract":"The corona virus outbreak first occurred at the end of 2019 in Wuhan, China. This virus then spread rapidly throughout the world, including Indonesia. So, countries in the world take steps to lock down. In Indonesia, large-scale social restrictions have been implemented in big cities with a high prevalence of the spread of the virus. Efforts to control the virus include limiting mobility, wearing masks, frequently washing hands or using hand sanitizers. Hand sanitizers are products made from ethanol which are increasingly limited and expensive during the Covid-19 pandemic. The purpose of this study is to make bioethanol with a variation of the fermentation time made from molasses, so as to produce ethanol content above 70%. This research is an experimental research. Production of bioethanol based on molasses, aquades, NPK, urea, and yeast culture of Saccharomycess cerevisae with time variations of 4, 5, and 6 days and distilled at a temperature of 500C. Testing the ethanol content using gas chromatography mass spectrometry. The results of this study, the fourth day of fermentation produces ethanol content of 50.23±0.42% with an average volume of 490 mL; day 5 produced ethanol content of 62.92±0.38% with an average volume of 385 mL; and the 6th day yielded ethanol content of 72.11±0.46% with an average volume of 243 mL. So, the 6th day of fermentation is the best fermentation to produce bioethanol as a raw material for hand sanitizer with ethanol content above 70%.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87980038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRAK Nutrient agar merupakan jenis media umum yang sering digunakan pada laboratorium, harga media ini relatif mahal. Nutrient agar berbentuk padat, terdiri atas campuran ekstrak daging, pepton serta agar. Ikan teri jengki dan ikan layang deles jumlahnya melimpah di Indonesia. Ikan teri jengki (Stolephorus indicus) mengandung protein 51.54%, sementara ikan layang deles (Decapterus Macrosoma) mengandung protein sebesar 57,89%. Escherichia coli digunakan sebab bakteri ini merupakan salah satu jenis mikroorganisme kontrol positif yang direkomendasikan berdasarkan Data Sheet Nutrient Agar OXOID. Penelitian ini bersifat eksperimental dengan tiga perlakuan pada masing-masing bahan yaitu variasi massa 3 gram, 4 gram dan 5 gram yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya. Hasil penelitian menunjukkan jumlah koloni rata-rata Escherichia coli yang tumbuh pada kontrol positif sebanyak 118 x 1013, sementara pada media ikan teri jengki variasi massa 3 gram adalah 72 x 1013 CFU/mL, 4 gram sebanyak 85 x 1013 CFU/mL, 5 gram sebanyak 94 x 1013 CFU/mL. Pada media ikan layang deles variasi 3 gram sebanyak 88 x 1013 CFU/mL, 4 gram sebanyak 96 x 1013 CFU/mL, 5 gram 108 x 1013 CFU/mL. Variasi massa yang paling baik dan mendekati hasil kontrol positif (nutriet agar) pada ikan teri jengki dan ikan layang deles adalah variasi massa 5 gram.Kata kunci: ikan teri jengki, ikan layang deles, Escherichia coli, nutrient agar
{"title":"Pemanfaatan Teri Jengki (Stolephorus indicus) Dan Layang Deles (Decapterus macrosoma) Sebagai Media Alternatif Pertumbuhan Escherichia coli","authors":"Armelia Gitasari Nurhasanah, Pestariati Pestariati, Anita Dwi Anggraini, Sri Sulami Endah Astuti","doi":"10.30651/jmlt.v6i1.14452","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.14452","url":null,"abstract":"ABSTRAK Nutrient agar merupakan jenis media umum yang sering digunakan pada laboratorium, harga media ini relatif mahal. Nutrient agar berbentuk padat, terdiri atas campuran ekstrak daging, pepton serta agar. Ikan teri jengki dan ikan layang deles jumlahnya melimpah di Indonesia. Ikan teri jengki (Stolephorus indicus) mengandung protein 51.54%, sementara ikan layang deles (Decapterus Macrosoma) mengandung protein sebesar 57,89%. Escherichia coli digunakan sebab bakteri ini merupakan salah satu jenis mikroorganisme kontrol positif yang direkomendasikan berdasarkan Data Sheet Nutrient Agar OXOID. Penelitian ini bersifat eksperimental dengan tiga perlakuan pada masing-masing bahan yaitu variasi massa 3 gram, 4 gram dan 5 gram yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya. Hasil penelitian menunjukkan jumlah koloni rata-rata Escherichia coli yang tumbuh pada kontrol positif sebanyak 118 x 1013, sementara pada media ikan teri jengki variasi massa 3 gram adalah 72 x 1013 CFU/mL, 4 gram sebanyak 85 x 1013 CFU/mL, 5 gram sebanyak 94 x 1013 CFU/mL. Pada media ikan layang deles variasi 3 gram sebanyak 88 x 1013 CFU/mL, 4 gram sebanyak 96 x 1013 CFU/mL, 5 gram 108 x 1013 CFU/mL. Variasi massa yang paling baik dan mendekati hasil kontrol positif (nutriet agar) pada ikan teri jengki dan ikan layang deles adalah variasi massa 5 gram.Kata kunci: ikan teri jengki, ikan layang deles, Escherichia coli, nutrient agar","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76206080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.30651/jmlt.v6i1.11932
Retno Sulistiyowati, Agny Kusumaningrum
Asam urat merupakan senyawa kimia hasil akhir dari metabolisme purin dalam tubuh. Wanita mengalami hiperurisemia meningkat pada wanita setelah menopause, karena mengalami penurunan hormon estrogen. Daun sirsak mengandung flavonoid yang bersifat sebagai antioksidan yang dapat mengurangi terbentuknya asam urat. Tujuan penelitian menganalisis pengaruh pemberian air rebusan daun sirsak terhadap kadar asam urat pada wanita usia di atas 45 tahun di Desa Besuki. Penelitian berjenis kuantitatif dengan menggunakan metode pre-experimental design tipe one group pretest – posttest. 14 responden mengkonsumsi air rebusan daun sirsak sebanyak satu gelas sehari selama 2 minggu. Hasil penelitian menunjukkan penurunan kadar asam urat setelah pemberian air rebusan daun sirsak, dengan rerata kadar asam urat sebelum diberi perlakuan 6,0714±1,06946 mg/dL sedangkan rerata kadar asam urat setelah diberi perlakuan 5,4071±1,36971 mg/dL. Rerata nilai perbedaan kadar asam urat sebelum dan sesudah diberi perlakuan sebesar 0,6429±0,72707 mg/dL.
{"title":"Pengaruh Air Rebusan Daun Sirsak (Annona muricata Linn) Terhadap Kadar Asam Urat Pada Wanita Usia Lebih Dari 45 Tahun Di Desa Besuki Kecamatan Lumbir Kabupaten Banyumas","authors":"Retno Sulistiyowati, Agny Kusumaningrum","doi":"10.30651/jmlt.v6i1.11932","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.11932","url":null,"abstract":"Asam urat merupakan senyawa kimia hasil akhir dari metabolisme purin dalam tubuh. Wanita mengalami hiperurisemia meningkat pada wanita setelah menopause, karena mengalami penurunan hormon estrogen. Daun sirsak mengandung flavonoid yang bersifat sebagai antioksidan yang dapat mengurangi terbentuknya asam urat. Tujuan penelitian menganalisis pengaruh pemberian air rebusan daun sirsak terhadap kadar asam urat pada wanita usia di atas 45 tahun di Desa Besuki. Penelitian berjenis kuantitatif dengan menggunakan metode pre-experimental design tipe one group pretest – posttest. 14 responden mengkonsumsi air rebusan daun sirsak sebanyak satu gelas sehari selama 2 minggu. Hasil penelitian menunjukkan penurunan kadar asam urat setelah pemberian air rebusan daun sirsak, dengan rerata kadar asam urat sebelum diberi perlakuan 6,0714±1,06946 mg/dL sedangkan rerata kadar asam urat setelah diberi perlakuan 5,4071±1,36971 mg/dL. Rerata nilai perbedaan kadar asam urat sebelum dan sesudah diberi perlakuan sebesar 0,6429±0,72707 mg/dL.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85630178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.30651/jmlt.v6i1.15840
Sarlince Agustin Nesan, B. Santosa, Mudyawati Kamarudin
Malaria is almost found all over the world, including Indonesia. WHO data on malaria case transmission in 2020, namely 241 million and 627,000 deaths. Female Anopheles mosquitoes are the main factor in the transmission of parasites, one of which is Plasmodium falciparum which is most dangerous to cause complications to death. Data from the Ministry of Health 2019 the highest transmission in Papua Province is 216,380 cases. The malaria elimination program uses ACT as a treatment therapy for malaria. The first appearance of ACT resistance in Cambodia was in 2008 to Plasmodium falciparum which was associated with the presence of a Kelch 13 gene mutation. The purpose of this study was to identify a mutation of the Kelch 13 gene markers of resistance in Plasmodium falciparum with ACT treatment after 3 days. This type of exploratory study used a population of all patients at the Amban Manokwari Health Center in West Papua, with a total sampling in August-September 2022. Of the 51 and 11 H3 samples met the inclusion criteria using RDT and Microscopic methods. The DNA isolated sample using Favorgen kit then amplified PCR and electrophoresis gel agarose. Then were 7 amplicons seen in the DNA band (±200bp) and confirmed results from PT. Science Genetics (±100bp), then 1 amplicon is continued for sequencing. The sequence results were analyzed using the Bioedit and Mega IX softwere with the alignment of the sequence results. Changes in nucleeid bases and amino acids were obtained so that the mutation of the Kelch 13 gene occurred, 3 mutation variants: substitution (transition & transversion), silent mutation (C3T codon 1 cysteine-cysteine), and missense mutation (T4A codon 2 tryptophan-serine). It can be concluded that the Plasmodium falciparum treatment ACT carriers the Kelch 13 gene mutation as one of the markers of resistance. Keywords: ACT, Plasmodium falciparum, Kelch 13 Gene Mutation.
{"title":"Identifikasi Mutasi Gen kelch 13 Penanda Resistensi Pada Plasmodium falciparum Dengan Pengobatan ACT Setelah 3 Hari Di Manokwari Papua Barat","authors":"Sarlince Agustin Nesan, B. Santosa, Mudyawati Kamarudin","doi":"10.30651/jmlt.v6i1.15840","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.15840","url":null,"abstract":"Malaria is almost found all over the world, including Indonesia. WHO data on malaria case transmission in 2020, namely 241 million and 627,000 deaths. Female Anopheles mosquitoes are the main factor in the transmission of parasites, one of which is Plasmodium falciparum which is most dangerous to cause complications to death. Data from the Ministry of Health 2019 the highest transmission in Papua Province is 216,380 cases. The malaria elimination program uses ACT as a treatment therapy for malaria. The first appearance of ACT resistance in Cambodia was in 2008 to Plasmodium falciparum which was associated with the presence of a Kelch 13 gene mutation. The purpose of this study was to identify a mutation of the Kelch 13 gene markers of resistance in Plasmodium falciparum with ACT treatment after 3 days. This type of exploratory study used a population of all patients at the Amban Manokwari Health Center in West Papua, with a total sampling in August-September 2022. Of the 51 and 11 H3 samples met the inclusion criteria using RDT and Microscopic methods. The DNA isolated sample using Favorgen kit then amplified PCR and electrophoresis gel agarose. Then were 7 amplicons seen in the DNA band (±200bp) and confirmed results from PT. Science Genetics (±100bp), then 1 amplicon is continued for sequencing. The sequence results were analyzed using the Bioedit and Mega IX softwere with the alignment of the sequence results. Changes in nucleeid bases and amino acids were obtained so that the mutation of the Kelch 13 gene occurred, 3 mutation variants: substitution (transition & transversion), silent mutation (C3T codon 1 cysteine-cysteine), and missense mutation (T4A codon 2 tryptophan-serine). It can be concluded that the Plasmodium falciparum treatment ACT carriers the Kelch 13 gene mutation as one of the markers of resistance. Keywords: ACT, Plasmodium falciparum, Kelch 13 Gene Mutation.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86879473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil sludge is a product of petroleum mining activities and causes environmental pollution. However, oil sludge, including hazardous and toxic waste materials (B3), has been less effective in reducing oil sludge pollution. Thus, the researcher solved the issue by using hydrocarbonoclastic. It is necessary to use hydrocarbonoclastic bacteria isolated directly from their habitat (indigenous bacteria) as hydrocarbon degrading agents. Therefore, this study aimed to isolate and identify indigenous thermophilic bacteria from East Kalimantan Oil sludge. This study is an observational study that is analyzed descriptively. Procedure for isolation and identification of thermophilic bacteria from oil sludge grow on Synthetic Mineral Water media (SMW) with and without an autoclave. The Microbact Identification System Kit GNB 24E was used to characterize colonies macroscopically, microscopically, Gram staining, physiological tests (catalase, coagulase, and motility tests), and biochemically. The bacteria that were successfully isolated were later identified with Microbact Software and Bergey's book Manual of Determinative Bacteriology Ninth Edition. The results of the isolation and identification of thermophilic Indigenous bacteria from Oil Sludge Kalimantan Timur found Pseudomonas aeruginosa species with a similarity accuracy of 98.33%. The identified bacterial isolates can later be used as bioremediation agents on soils polluted with oil sludge. Keywords : Indigenous bacteria, Oil Sludge, Pseudomonas aeruginosa.
油泥是石油开采活动的产物，对环境造成污染。然而，包括有害和有毒废物(B3)在内的油泥在减少油泥污染方面效果不佳。因此，研究人员利用油气碎屑解决了这一问题。有必要使用直接从其栖息地分离的破烃菌(原生细菌)作为烃类降解剂。因此，本研究旨在从东加里曼丹油泥中分离和鉴定本地嗜热细菌。本研究是一项描述性分析的观察性研究。从合成矿泉水介质(SMW)上生长的油泥中分离和鉴定嗜热细菌的程序，有和没有高压灭菌器。使用microbactidentification System Kit GNB 24E对菌落进行宏观、微观、革兰氏染色、生理测试(过氧化氢酶、凝固酶和运动性测试)和生化表征。成功分离的细菌后来用microbacact软件和Bergey的书《测定细菌学手册》第九版进行了鉴定。加里曼丹铁木尔油泥中嗜热原生细菌的分离鉴定结果为铜绿假单胞菌，相似度为98.33%。鉴定出的分离菌可作为油泥污染土壤的生物修复剂。关键词:原生细菌;油泥;铜绿假单胞菌;
{"title":"Isolasi Dan Karakterisasi Bakteri Termofilik Dari Oil Sludge Asal Kalimantan Timur","authors":"Mulya Fitrah Juniawan, Dita Artanti, Yuni Gayatri, Ainutajriani Ainutajriani","doi":"10.30651/jmlt.v6i1.15898","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.15898","url":null,"abstract":"Oil sludge is a product of petroleum mining activities and causes environmental pollution. However, oil sludge, including hazardous and toxic waste materials (B3), has been less effective in reducing oil sludge pollution. Thus, the researcher solved the issue by using hydrocarbonoclastic. It is necessary to use hydrocarbonoclastic bacteria isolated directly from their habitat (indigenous bacteria) as hydrocarbon degrading agents. Therefore, this study aimed to isolate and identify indigenous thermophilic bacteria from East Kalimantan Oil sludge. This study is an observational study that is analyzed descriptively. Procedure for isolation and identification of thermophilic bacteria from oil sludge grow on Synthetic Mineral Water media (SMW) with and without an autoclave. The Microbact Identification System Kit GNB 24E was used to characterize colonies macroscopically, microscopically, Gram staining, physiological tests (catalase, coagulase, and motility tests), and biochemically. The bacteria that were successfully isolated were later identified with Microbact Software and Bergey's book Manual of Determinative Bacteriology Ninth Edition. The results of the isolation and identification of thermophilic Indigenous bacteria from Oil Sludge Kalimantan Timur found Pseudomonas aeruginosa species with a similarity accuracy of 98.33%. The identified bacterial isolates can later be used as bioremediation agents on soils polluted with oil sludge. Keywords : Indigenous bacteria, Oil Sludge, Pseudomonas aeruginosa.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82695973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jombang Regency was the 10th most prevalent smoker in East Java in 2018 and experienced an increase in the prevalence of smokers by 0.1% from 2013. The high number of smokers raises a concern. Smoking may lead to an increase of hs-CRP and LDL cholesterol levels, where the increase in these two parameters is closely related to the formation of atherosclerotic plaques, as a cause of cardiovascular disease. Physical activity of each individual is something that needs to be known because it affects the levels of hs-CRP and LDL cholesterol. This study aims to analyze the correlation between hs-CRP and LDL cholesterol in active smokers with physical activity. This study used an observational analytic study with a cross-sectional design. This research was conducted in March-April 2022 with a total sample of 32 active smokers who work as pedicab drivers at Pondok Pesantren 'DU', Jombang Regency. The hs-CRP examination was carried out using the Latex Turbidimetric Assay method, while the LDL cholesterol examination was using the Homogeneous Enzymatic Colorimetric Assay method. Based on the statistical analysis of the Spearman correlation, the results obtained p>0.05 (p = 0.687, r = 0.074), which means that there is no correlation between levels of hs-CRP and LDL cholesterol in active smokers with physical activity.
2018年，仲邦摄政是东爪哇第十大吸烟者，吸烟者的患病率比2013年增加了0.1%。吸烟人数之多令人担忧。吸烟可导致hs-CRP和LDL胆固醇水平升高，其中这两个参数的升高与动脉粥样硬化斑块的形成密切相关，是引起心血管疾病的原因之一。每个人的身体活动都是需要了解的，因为它会影响hs-CRP和LDL胆固醇的水平。本研究旨在分析活跃吸烟者hs-CRP和LDL胆固醇与体力活动的相关性。本研究采用横断面设计的观察性分析研究。这项研究是在2022年3月至4月期间进行的，共有32名在中邦县Pondok Pesantren 'DU'担任三轮车司机的活跃吸烟者。hs-CRP检测采用乳胶比浊法，LDL -胆固醇检测采用均质酶比色法。通过Spearman相关性的统计分析，结果得到p>0.05 (p = 0.687, r = 0.074)，即活跃吸烟者hs-CRP和LDL胆固醇水平与体力活动无相关性。
{"title":"Korelasi Kadar High Sensitivity C-Reactive Protein (hs-CRP) Dan Kolesterol Low Density Lipoprotein (LDL) Pada Perokok Aktif Dengan Aktivitas Fisik","authors":"Aliya Ayu Rizqiyah, Evy Diah Woelansari, Suhariyadi Suhariyadi","doi":"10.30651/jmlt.v6i1.14363","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.14363","url":null,"abstract":"Jombang Regency was the 10th most prevalent smoker in East Java in 2018 and experienced an increase in the prevalence of smokers by 0.1% from 2013. The high number of smokers raises a concern. Smoking may lead to an increase of hs-CRP and LDL cholesterol levels, where the increase in these two parameters is closely related to the formation of atherosclerotic plaques, as a cause of cardiovascular disease. Physical activity of each individual is something that needs to be known because it affects the levels of hs-CRP and LDL cholesterol. This study aims to analyze the correlation between hs-CRP and LDL cholesterol in active smokers with physical activity. This study used an observational analytic study with a cross-sectional design. This research was conducted in March-April 2022 with a total sample of 32 active smokers who work as pedicab drivers at Pondok Pesantren 'DU', Jombang Regency. The hs-CRP examination was carried out using the Latex Turbidimetric Assay method, while the LDL cholesterol examination was using the Homogeneous Enzymatic Colorimetric Assay method. Based on the statistical analysis of the Spearman correlation, the results obtained p>0.05 (p = 0.687, r = 0.074), which means that there is no correlation between levels of hs-CRP and LDL cholesterol in active smokers with physical activity.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"240 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75849130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.30651/jmlt.v6i1.17996
Umi Ma’rifah, Baterun Kunsah, Nova Elok Mardiyah, Fulatul Anifah
The increasing number of Covid-19 cases, especially the presence of a new Delta variant which causes a risk of severe symptoms in pregnant women, the Indonesian government has established a screening program and implementation of the Covid-19 vaccination as stated in circular letter HK.02.01/I/2007/2021. Based on preliminary studies carried out on pregnant women in the city of Surabaya, there are 90% percent of pregnant women who are still hesitant and afraid to vaccinate. One form of effort being made is the development of an application that facilitates screening and accelerates vaccination of pregnant women, namely the VMil application. The purpose of this study was to find out how the Volunteer program through the V-MiL application affects pregnant women in carrying out Covid-19. This type of research is pre-experimental with one group pre-test and post test design. The dependent variable is the respondent's participation in the implementation of the Covid-19 vaccine, while the independent variable is intervention by volunteers using the V-MiL application. The research sample for pregnant women who had not been vaccinated was 40 people using a purposive sampling technique. Data collection with questionnaires and interviews. The results of the study the majority of respondents aged 21-30 years (58%), gestational age 13-27 weeks (78%). After the intervention the respondents had good knowledge 18 (45%), lack of knowledge 22 (55%), negative attitude positive attitude 16 (40%), negative attitude 24 (60%), strong motivation 15 (38%), weak motivation 25 (63%) to vaccinate. Respondents did the Covid-19 vaccine as many as 15 (38%) and 25 (63%) respondents did not do the vaccine. Analysis using the Paired sample T test obtained a value of λ of 0.001 <0.005 which indicated that there was a significant influence on the intervention given by volunteers to the respondents. Keywords : Volunteers, Covid-19 Vaccines, Pregnant Women
{"title":"Volunteer Program Skrining Dan Percepatan Vaksinasi Covid-19 Pada Ibu Hamil Di Surabaya","authors":"Umi Ma’rifah, Baterun Kunsah, Nova Elok Mardiyah, Fulatul Anifah","doi":"10.30651/jmlt.v6i1.17996","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.17996","url":null,"abstract":"The increasing number of Covid-19 cases, especially the presence of a new Delta variant which causes a risk of severe symptoms in pregnant women, the Indonesian government has established a screening program and implementation of the Covid-19 vaccination as stated in circular letter HK.02.01/I/2007/2021. Based on preliminary studies carried out on pregnant women in the city of Surabaya, there are 90% percent of pregnant women who are still hesitant and afraid to vaccinate. One form of effort being made is the development of an application that facilitates screening and accelerates vaccination of pregnant women, namely the VMil application. The purpose of this study was to find out how the Volunteer program through the V-MiL application affects pregnant women in carrying out Covid-19. This type of research is pre-experimental with one group pre-test and post test design. The dependent variable is the respondent's participation in the implementation of the Covid-19 vaccine, while the independent variable is intervention by volunteers using the V-MiL application. The research sample for pregnant women who had not been vaccinated was 40 people using a purposive sampling technique. Data collection with questionnaires and interviews. The results of the study the majority of respondents aged 21-30 years (58%), gestational age 13-27 weeks (78%). After the intervention the respondents had good knowledge 18 (45%), lack of knowledge 22 (55%), negative attitude positive attitude 16 (40%), negative attitude 24 (60%), strong motivation 15 (38%), weak motivation 25 (63%) to vaccinate. Respondents did the Covid-19 vaccine as many as 15 (38%) and 25 (63%) respondents did not do the vaccine. Analysis using the Paired sample T test obtained a value of λ of 0.001 <0.005 which indicated that there was a significant influence on the intervention given by volunteers to the respondents. Keywords : Volunteers, Covid-19 Vaccines, Pregnant Women","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78396430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.30651/jmlt.v6i1.14494
C. S. Rini
Saluran pernapasan merupakan organ tubuh manusia yang banyak mengandung populasi bakteri (sekitar 13 spesies) yang dapat menjadi patogen seperti Klebsiella pneumoniae dan Streptococcus pneumoniae. Oleh karena itu, perlu adanya upaya mengendalikan Klebsiella pneumoniae dan Streptococcus pneumoniae dengan bahan dari bahan alam, salah satunya yaitu pare (Momordica charantia Linn). Buah pare (Momordica charantia Linn) telah terbukti khasiatnya sebagai obat yang memiliki kandungan senyawa seperti alkaloid, saponin, fenolik, streoid, flavonoid, dan tanin yang memiliki kemampuan sebagai antibakteri. Jenis penelitian ini adalah penelitian eksperimental. Dengan metode difusi kertas cakram atau Kirby-Bauer dengan 5 konsentrasi yaitu : 20%, 40%, 60%, 80%, 100%, dan digunakan antibiotik kloramfenikol sebagai (kontrol+). Pengujian dilakukan sebanyak 3 kali pengulangan. Data yang peroleh kemudian diuji menggunakan uji Anova one way dengan nilai signifikansi 0,05. Hasil penelitian menunjukkan bahwa ekstrak segar buah pare efektif terhadap pertumbuhan bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae. Konsentrasi optimum pada bakteri Klebsiella pneumoniae diperoleh pada konsentrasi 100% dengan diameter sebesar 21,31 mm dan konsentrasi 80% dengan diameter sebesar 22,36 mm pada bakteri Streptococcus pneumoniae, sedangkan hasil uji ANOVA one way yang telah dilakukan terhadap diameter zona hambat bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae diperoleh nilai signifikan p=0,000 dari hasil tersebut diketahui bahwa nilai <0,05 yang menunjukkan ada pengaruh yang nyata dari pemberian ekstrak segar buah pare terhadap pertumbuhan bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae.
{"title":"Uji Daya Hambat Ekstrak Segar Buah Pare (Momordica charantia Linn.) Terhadap Pertumbuhan Bakteri Klebsiella pneumoniae Dan Streptococcus pneumoniae","authors":"C. S. Rini","doi":"10.30651/jmlt.v6i1.14494","DOIUrl":"https://doi.org/10.30651/jmlt.v6i1.14494","url":null,"abstract":"Saluran pernapasan merupakan organ tubuh manusia yang banyak mengandung populasi bakteri (sekitar 13 spesies) yang dapat menjadi patogen seperti Klebsiella pneumoniae dan Streptococcus pneumoniae. Oleh karena itu, perlu adanya upaya mengendalikan Klebsiella pneumoniae dan Streptococcus pneumoniae dengan bahan dari bahan alam, salah satunya yaitu pare (Momordica charantia Linn). Buah pare (Momordica charantia Linn) telah terbukti khasiatnya sebagai obat yang memiliki kandungan senyawa seperti alkaloid, saponin, fenolik, streoid, flavonoid, dan tanin yang memiliki kemampuan sebagai antibakteri. Jenis penelitian ini adalah penelitian eksperimental. Dengan metode difusi kertas cakram atau Kirby-Bauer dengan 5 konsentrasi yaitu : 20%, 40%, 60%, 80%, 100%, dan digunakan antibiotik kloramfenikol sebagai (kontrol+). Pengujian dilakukan sebanyak 3 kali pengulangan. Data yang peroleh kemudian diuji menggunakan uji Anova one way dengan nilai signifikansi 0,05. Hasil penelitian menunjukkan bahwa ekstrak segar buah pare efektif terhadap pertumbuhan bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae. Konsentrasi optimum pada bakteri Klebsiella pneumoniae diperoleh pada konsentrasi 100% dengan diameter sebesar 21,31 mm dan konsentrasi 80% dengan diameter sebesar 22,36 mm pada bakteri Streptococcus pneumoniae, sedangkan hasil uji ANOVA one way yang telah dilakukan terhadap diameter zona hambat bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae diperoleh nilai signifikan p=0,000 dari hasil tersebut diketahui bahwa nilai <0,05 yang menunjukkan ada pengaruh yang nyata dari pemberian ekstrak segar buah pare terhadap pertumbuhan bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91088498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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