[Effect of pesticides on the release of histamine, chemotactic factors and leukotrienes from rat mast cells and human basophils].

Abstract

The influence of pesticides on mediator release from rat peritoneal mast cells and human basophiles was studied. Mediators from mast cells and basophils are important factors in allergic and inflammatory reactions. Release of histamine from rat mast cells and human basophils is demonstrated by stimulation with dieldrin, DDT, heptachlor, heptachlorepoxid and biphenyl. This pesticide-induced histamine secretion is dose-dependent and requires Ca2+. In contrast, incubation with gamma-HCH, HCB and carbaryl produces no significant histamine release. Additive histamine secretion results from simultaneous stimulation of rat mast cells with pesticides and anaphylatoxin C5a. Further, secretion of eosinophil and neutrophil chemotactic factors from rat mast cells is induced during incubation with dieldrin, biphenyl and heptachlorepoxid. It is also demonstrated that pesticides can stimulate the generation of lipidmediators. Biphenyl, gamma-HCH, heptachlor, heptachlorepoxid, DDT and dieldrin cause the release of leukotriene C4 from rat mast cells. The possible role of these in vitro results is discussed.