"MicroRNA Profiling of Plasma Exosomes in Type 2 Diabetes Following Exercises"

Abstract

A total of 12 male participants, including 7 patients and 5 healthy individuals, were recruited. T2D patients underwent a randomized three-period crossover 1-hour postprandial intervention: high-intensity interval exercise (T2D-HIIE), moderate-intensity continuous exercise (T2D-MICE) with cycling and sedentary control (T2D-CON) for 30 min, while healthy participants served as controls (HP-CON) without exercise intervention. Exosomes were extracted from plasma using differential ultracentrifugation at 1.5 hours postprandial, which equates to immediate post-exercise for T2D patients. Exosomal miRNA sequencing was conducted. KEGG analysis was performed to identify pathways linked with altered expression of miRNAs.