Triploidy verification of Schizothorax richardsonii using erythrocyte cell and nucleus measurement

Abstract

The individuals of diploid and triploid Schizothorax richardsonii (Gray, 1832) were identified using erythrocyte indices. The Erythrocyte cell's major axis of the triploid was 17% higher than the diploid. The increase in the nucleus major axis size of the erythrocyte of the triploid was also greater by 8% concerning the diploid fishes. The erythrocyte measurement ranged between 13.29±1.06 to 14.45±0.97 for diploid 15.91±1.32 to 16.49±1.23 for triploid erythrocyte nucleus 6.16±0.67 to 6.40±0.63 and 6.70±0.71 to 6.94±0.56 for diploid and triploid respectively. The data were analyzed by One Way of Analysis of Variance (ANOVA) for both diploid (control) and triploid (treated) erythrocyte and nucleus measurement which showed significant differences with p<0.05. These parameters, thus, can successfully be used in discriminating diploid and triploid S. richadrsonii.