Isolation of Lactiplantibacillus plantarum producing Extracellular Lipase from Dairy Products and Optimization of the Enzyme Production

IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Research Journal of Biotechnology Pub Date : 2023-12-05 DOI:10.25303/1901rjbt0109
Mahdiye Ziyaee, Somaye Imanparast, Fatemeh Salimi, H. Jahandar
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Abstract

Recently, microbial lipases isolated from probiotic bacteria have attracted the attention of many scientists because they have significantly beneficial properties for human health. As a highly nutritious substrate, dairy products possess great potential for probiotic bacteria isolation. The main purpose of this study was to isolate probiotic bacteria in order to search for lipase producing bacteria. The bacteria were isolated from dairy products and purified by repeated sub-culturing on MRS medium at 37°C. The isolates were subjected to primary and secondary screening via fluorometric and colorimetric methods respectively. The isolates showing orange fluorescence under UV light at 350 nm around their colonies were selected for the colorimetric method. In this method, lipase activity was investigated by p-nitrophenyl palmitate. Probiotic characterizations of lipase-producing isolates were performed by acid resistance test, cell surface hydrophobicity assay, auto-aggregation and co-aggregation. The optimization of lipase production was performed by Central Composite Design (CCD). In the present study, 92 isolates were recovered from 71 samples. The results showed that 41 isolates were positive for fluorometric assay. According to the amount of enzyme production, seven strains were selected for probiotic characterizations. Finally, a potential probiotic strain of genus Lactobacillus showed the highest enzyme activity with 3.4 U.ml-1 compared to other isolates. This strain showed 100% similarity to Lactiplantibacillus plantarum. This research indicated that the lipase of Lactiplantibacillus plantarum can be used as a promising enzyme in the food and medicine industries.
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从乳制品中分离出产生胞外脂肪酶的植物乳杆菌并优化酶的生产
近来,从益生菌中分离出的微生物脂肪酶引起了许多科学家的关注,因为它们对人体健康大有裨益。乳制品作为一种高营养基质,具有分离益生菌的巨大潜力。本研究的主要目的是分离益生菌,以寻找产生脂肪酶的细菌。研究人员从乳制品中分离出益生菌,并通过在 37°C 的 MRS 培养基上反复亚培养进行纯化。分离菌分别通过荧光法和比色法进行初筛和复筛。比色法筛选在 350 纳米紫外光下菌落周围显示橙色荧光的分离物。在这种方法中,脂肪酶活性是通过对硝基苯棕榈酸酯来研究的。通过耐酸性测试、细胞表面疏水性检测、自动聚集和共聚集,对产生脂肪酶的分离菌进行了益生特性鉴定。脂肪酶产量的优化是通过中央复合设计(CCD)进行的。本研究从 71 份样本中回收了 92 个分离菌株。结果显示,41 个分离菌株对荧光测定呈阳性。根据产酶量,7 株菌株被选为益生菌。最后,乳酸杆菌属的一个潜在益生菌株与其他分离株相比,酶活性最高,达到 3.4 U.ml-1。该菌株与植物乳杆菌的相似度为 100%。这项研究表明,植物乳杆菌的脂肪酶可作为一种有前途的酶用于食品和医药行业。
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来源期刊
Research Journal of Biotechnology
Research Journal of Biotechnology 工程技术-生物工程与应用微生物
CiteScore
0.60
自引率
0.00%
发文量
192
审稿时长
1.5 months
期刊介绍: We invite you to contribute Research Papers / Short Communications / Review Papers: -In any field of Biotechnology, Biochemistry, Microbiology and Industrial Microbiology, Soil Technology, Agriculture Biotechnology. -in any field related to Food Biotechnology, Nutrition Biotechnology, Genetic Engineering and Commercial Biotechnology. -in any field of Biotechnology related to Drugs and Pharmaceutical products for human beings, animals and plants. -in any field related to Environmental Biotechnolgy, Waste Treatment of Liquids, Soilds and Gases; Sustainability. -in inter-realted field of Chemical Sciences, Biological Sciences, Environmental Sciences and Life Sciences. -in any field related to Biotechnological Engineering, Industrial Biotechnology and Instrumentation. -in any field related to Nano-technology. -in any field related to Plant Biotechnology.
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