{"title":"Other Sea Star Igkappa Gene Cloning Assay in E.Coli with New Parameters","authors":"","doi":"10.33140/jcei.08.02.02","DOIUrl":null,"url":null,"abstract":"The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014 [1]. This construct is designed to allow the expression of a 13.6 kDA protein with a C-terminal 6histag. It is supposed to be an anti-HRP (Horse-radish peroxydase protein). This protein was not expressed in first E.coli: we attempt to explain this phenomenon.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"112 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical & experimental immunology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33140/jcei.08.02.02","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014 [1]. This construct is designed to allow the expression of a 13.6 kDA protein with a C-terminal 6histag. It is supposed to be an anti-HRP (Horse-radish peroxydase protein). This protein was not expressed in first E.coli: we attempt to explain this phenomenon.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
