ESTIMATION AND DETOXIFICATION OF AFLATOXINS IN OSTRICH FEED SAMPLES COLLECTED FROM LAHORE, PAKISTAN

None Sonal Chaudhry, None Bushra Nisar Khan, None Sajila Hina, None Waqar Ali Gill, None Ayesha Shaheen, None Romana Zulfiqar, None Madiha Ashraf
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Abstract

Ostrich farming is an emerging agricultural business in Pakistan with high demands for meat, eggs, oil, feathers, and leather. However, the presence of aflatoxins may lead to health hazards to poultry and consumers depending on this industry. A study for aflatoxin contamination was conducted on ostrich feed samples collected from 10 localities of Lahore with an emphasis on storage and handling conditions. The samples were analyzed using thin liquid chromatography. The aflatoxin positive feed samples were then detoxified using 0.5, 1, and 1.5 g bud powder of Moringa oleifera, incubated for 7, 14, 21, and 28 days, and were analyzed again. Results revealed maximum contamination (60%) during summers with an average temperature of 30.88 ºC and minimum (20%) in winters with an average temperature of 17.08 ºC. The major contaminant that found was B1 Aflatoxin. Results were statistically analyzed by applying Paired T–Test which proved the effectiveness of M. oleifera ratio in detoxification of feed aflatoxins. On the basis of this study, it was concluded that M. oleifera buds are an efficient biological agent against aflatoxins in feed. In addition, control of temperature and improvement in storage conditions at the sites can reduce the risks of aflatoxicosis
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从巴基斯坦拉合尔收集的鸵鸟饲料样品中黄曲霉毒素的估计和解毒
鸵鸟养殖在巴基斯坦是一项新兴的农业业务,对肉、蛋、油、羽毛和皮革的需求很高。然而,黄曲霉毒素的存在可能会对家禽和依赖该行业的消费者造成健康危害。对从拉合尔10个地区采集的鸵鸟饲料样本进行了黄曲霉毒素污染研究,重点研究了储存和处理条件。采用薄液相色谱法对样品进行分析。分别用0.5、1、1.5 g辣木芽粉对黄曲霉毒素阳性饲料进行解毒,孵育7、14、21、28 d,再次进行分析。结果表明,夏季污染最大(60%),平均气温为30.88ºC;冬季污染最小(20%),平均气温为17.08ºC。发现的主要污染物是B1黄曲霉毒素。采用配对t检验对结果进行统计分析,证实了油松菌比例对饲料中黄曲霉毒素的脱毒效果。在此基础上,得出油橄榄芽是一种有效的饲料黄曲霉毒素生物制剂的结论。此外,控制温度和改善储存条件可以降低黄曲霉中毒的风险
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