Pub Date : 2023-09-25DOI: 10.54692/lgujls.2023.0703283
None Sonal Chaudhry, None Bushra Nisar Khan, None Sajila Hina, None Waqar Ali Gill, None Ayesha Shaheen, None Romana Zulfiqar, None Madiha Ashraf
Ostrich farming is an emerging agricultural business in Pakistan with high demands for meat, eggs, oil, feathers, and leather. However, the presence of aflatoxins may lead to health hazards to poultry and consumers depending on this industry. A study for aflatoxin contamination was conducted on ostrich feed samples collected from 10 localities of Lahore with an emphasis on storage and handling conditions. The samples were analyzed using thin liquid chromatography. The aflatoxin positive feed samples were then detoxified using 0.5, 1, and 1.5 g bud powder of Moringa oleifera, incubated for 7, 14, 21, and 28 days, and were analyzed again. Results revealed maximum contamination (60%) during summers with an average temperature of 30.88 ºC and minimum (20%) in winters with an average temperature of 17.08 ºC. The major contaminant that found was B1 Aflatoxin. Results were statistically analyzed by applying Paired T–Test which proved the effectiveness of M. oleifera ratio in detoxification of feed aflatoxins. On the basis of this study, it was concluded that M. oleifera buds are an efficient biological agent against aflatoxins in feed. In addition, control of temperature and improvement in storage conditions at the sites can reduce the risks of aflatoxicosis
{"title":"ESTIMATION AND DETOXIFICATION OF AFLATOXINS IN OSTRICH FEED SAMPLES COLLECTED FROM LAHORE, PAKISTAN","authors":"None Sonal Chaudhry, None Bushra Nisar Khan, None Sajila Hina, None Waqar Ali Gill, None Ayesha Shaheen, None Romana Zulfiqar, None Madiha Ashraf","doi":"10.54692/lgujls.2023.0703283","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703283","url":null,"abstract":"Ostrich farming is an emerging agricultural business in Pakistan with high demands for meat, eggs, oil, feathers, and leather. However, the presence of aflatoxins may lead to health hazards to poultry and consumers depending on this industry. A study for aflatoxin contamination was conducted on ostrich feed samples collected from 10 localities of Lahore with an emphasis on storage and handling conditions. The samples were analyzed using thin liquid chromatography. The aflatoxin positive feed samples were then detoxified using 0.5, 1, and 1.5 g bud powder of Moringa oleifera, incubated for 7, 14, 21, and 28 days, and were analyzed again. Results revealed maximum contamination (60%) during summers with an average temperature of 30.88 ºC and minimum (20%) in winters with an average temperature of 17.08 ºC. The major contaminant that found was B1 Aflatoxin. Results were statistically analyzed by applying Paired T–Test which proved the effectiveness of M. oleifera ratio in detoxification of feed aflatoxins. On the basis of this study, it was concluded that M. oleifera buds are an efficient biological agent against aflatoxins in feed. In addition, control of temperature and improvement in storage conditions at the sites can reduce the risks of aflatoxicosis","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antimicrobial fabric is suitable for providing protective covering to the medicinal industry, army uniform, casual wear, and household furnishing as well as for general public usage. The environmentally friendly antibacterial finish was extracted from leaves of Azadirachata indica, Butea monosperma and Litchi chinensis trees leaves and then applied on 100% polyester. Before and after applying the antimicrobial finish, fabric property was checked. The antimicrobial finish was applied by the pad dry cure method and the finish was fixed by using of polyurethane binder. Under aesthetic properties, stiffness and surface appearance were checked. The stiffness of the fabric was checked by Shirley Stiffness tester and surface appearance was checked by using AATCC Technical Manual /2004 TM 124-2001 203 test method. The aesthetic properties (smoothness, surface appearance) of the antimicrobial finish had a positive effect on 100% polyester. Results showed thatusing an antimicrobial finish made from the leaves of A. indica , B. monosperma , and L. chinensis reduced the amount of bacteria on polyester fabric by 100% when compared to the control group. The result showed that antimicrobial finish increased the stiffness while smoothness appearance was decreased in case of A. indica and B. monosperma and increased in case of L. chinensis.
{"title":"Effect of Antimicrobial Finish on Aesthetic property of Polyester Fabric","authors":"None Shama Sadaf, None Shagufta Naz, None Wajeeha Salamat, None Zeeshan Ahmad, None Saima Sharif, None Komal Hassan, None Ayesha Saeed","doi":"10.54692/lgujls.2023.0703284","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703284","url":null,"abstract":"Antimicrobial fabric is suitable for providing protective covering to the medicinal industry, army uniform, casual wear, and household furnishing as well as for general public usage. The environmentally friendly antibacterial finish was extracted from leaves of Azadirachata indica, Butea monosperma and Litchi chinensis trees leaves and then applied on 100% polyester. Before and after applying the antimicrobial finish, fabric property was checked. The antimicrobial finish was applied by the pad dry cure method and the finish was fixed by using of polyurethane binder. Under aesthetic properties, stiffness and surface appearance were checked. The stiffness of the fabric was checked by Shirley Stiffness tester and surface appearance was checked by using AATCC Technical Manual /2004 TM 124-2001 203 test method. The aesthetic properties (smoothness, surface appearance) of the antimicrobial finish had a positive effect on 100% polyester. Results showed thatusing an antimicrobial finish made from the leaves of A. indica , B. monosperma , and L. chinensis reduced the amount of bacteria on polyester fabric by 100% when compared to the control group. The result showed that antimicrobial finish increased the stiffness while smoothness appearance was decreased in case of A. indica and B. monosperma and increased in case of L. chinensis.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135859261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Awareness of ethical values between humans and animals is gaining significant attention in recent years. It is reflecting society's attitude towards animals. This survey-based research aimed to assess the ethical awareness among students and delve into the intricate web of relationships between humans and animals. The study examined the extent to which students were familiar to ethical issues related to animal welfare. This survey study designed with the students from various academic disciplines, age groups, and backgrounds. A mixed-methods approach was used to explore student’s awareness, attitudes, and experiences concerning animals. The survey reveal the multifaceted nature of ethical awareness among students and it uncovers various factors influencing students' ethical behaviors towards animals, including cultural and societal norms, personal experiences, and educational exposure. It was concluded that the survey provides valuable insights into the ethical awareness among students concerning the relationship between humans and animals. It emphasized the need for comprehensive educational programs that not only inform students about animal ethics but also encourage empathy, compassion, and responsible stewardship
{"title":"Evaluation of Ethical Awareness among Students to Scrutinize the Intrinsic Relationship between Humans and Animals","authors":"None Nageen Hussain, None Mubashir Ahmad, None Abida Butt, None Amina Bashir","doi":"10.54692/lgujls.2023.0703286","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703286","url":null,"abstract":"Awareness of ethical values between humans and animals is gaining significant attention in recent years. It is reflecting society's attitude towards animals. This survey-based research aimed to assess the ethical awareness among students and delve into the intricate web of relationships between humans and animals. The study examined the extent to which students were familiar to ethical issues related to animal welfare. This survey study designed with the students from various academic disciplines, age groups, and backgrounds. A mixed-methods approach was used to explore student’s awareness, attitudes, and experiences concerning animals. The survey reveal the multifaceted nature of ethical awareness among students and it uncovers various factors influencing students' ethical behaviors towards animals, including cultural and societal norms, personal experiences, and educational exposure. It was concluded that the survey provides valuable insights into the ethical awareness among students concerning the relationship between humans and animals. It emphasized the need for comprehensive educational programs that not only inform students about animal ethics but also encourage empathy, compassion, and responsible stewardship","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135859258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The world's most popular stimulant, caffeine, accounts for 80% of all caffeine consumption. It is widely used as a component in pharmaceuticals due to its high antioxidant and nutrient content, including phenol and polyphenol. On the presence of caffeine drinks are categorised as caffeinated and decaffeinated. The present study was conducted to evaluate the effect of caffeinated and decaffeinated coffee on blood pressure among the young generation. This experimental study was conducted on 85 healthy individuals (both male and female, non hypertensive) between 18-28 years of age. Blood Pressure (BP) was measured by an automatic digital blood pressure monitor, before and after taking of coffee in 30, 60, and 90 minutes. Each participant ingested 100 mL of coffee prepared from 50 mg of caffeinated coffee in water. The consumption time of each individual was between 2- 4 minutes. It was noticed almost 65.7% participants were found normal while, a significant difference in 34.3% found in systolic blood pressure (SBP) and diastolic blood pressure (DBP) after the intake of caffeinated coffee at p-value (<0.001).An increase of4.4 mmHg and 5.02 mmHg was found in systolic blood pressure after 60, 90 minutes respectively from the normal that is <20mm Hg. While, a slight increase was noticed in DBP after taking caffeinated coffee. No significant difference were seen after the congestion of decaffeinated coffee among participants. It was concluded by the study that individual responses can vary. There was increase in BP after taking caffeinated coffee. There was no significant effect found after decaffeinated coffee. Some people might be more sensitive to caffeine and feel its effects more strongly, while others might not notice much difference. Additionally, any effects from this small amount of coffee are likely to wear off relatively quickly, within a few hours at most.
{"title":"EFFECT OF CAFFEINATED AND DECAFFEINATED COFFEE ON BLOOD PRESSURE","authors":"None Tahira Khurram, None Samina Khanum, None Asma Nadeem, None Usama Kiran, None Rashida Kazmi, None Ummara Rasheed","doi":"10.54692/lgujls.2023.0703281","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703281","url":null,"abstract":"The world's most popular stimulant, caffeine, accounts for 80% of all caffeine consumption. It is widely used as a component in pharmaceuticals due to its high antioxidant and nutrient content, including phenol and polyphenol. On the presence of caffeine drinks are categorised as caffeinated and decaffeinated. The present study was conducted to evaluate the effect of caffeinated and decaffeinated coffee on blood pressure among the young generation. This experimental study was conducted on 85 healthy individuals (both male and female, non hypertensive) between 18-28 years of age. Blood Pressure (BP) was measured by an automatic digital blood pressure monitor, before and after taking of coffee in 30, 60, and 90 minutes. Each participant ingested 100 mL of coffee prepared from 50 mg of caffeinated coffee in water. The consumption time of each individual was between 2- 4 minutes. It was noticed almost 65.7% participants were found normal while, a significant difference in 34.3% found in systolic blood pressure (SBP) and diastolic blood pressure (DBP) after the intake of caffeinated coffee at p-value (<0.001).An increase of4.4 mmHg and 5.02 mmHg was found in systolic blood pressure after 60, 90 minutes respectively from the normal that is <20mm Hg. While, a slight increase was noticed in DBP after taking caffeinated coffee. No significant difference were seen after the congestion of decaffeinated coffee among participants. It was concluded by the study that individual responses can vary. There was increase in BP after taking caffeinated coffee. There was no significant effect found after decaffeinated coffee. Some people might be more sensitive to caffeine and feel its effects more strongly, while others might not notice much difference. Additionally, any effects from this small amount of coffee are likely to wear off relatively quickly, within a few hours at most.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135859263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-25DOI: 10.54692/lgujls.2023.0703278
None Ramna Zia, None Zahra Arshad, None Hina Batool, None Kaneez Fatima, None Syed Abdul Qadir Shah, None Muhammad Zaid
The symptoms of COVID-19 are acute and mostly people recovered from these after a specific time period. Although, a considerable percentage of patients around the globe were affected but Per our knowledge, study of post covid- 19 associated mental health symptoms found scarce. The present study was organized to inspect the frequency of long-term anosmia, parosmia, odynophagia and associated mental health issues in the Pakistani population. A 5-month study was collected via an online questionnaire and phone calls among patients infected from March 2020 to February 2021. This study included a total of 124 patients with COVID-19 RT-PCR positive test. It was recorded by this survey that almost 13% of cases were with long-term anosmia, 81% with parosmia after short-term anosmia, and 79%with odynophagia. The level of depression and anxiety among patients suffering from long-term parosmia was significantly higher in participants than in anosmia. Most shockingly, a large fraction of respondents showed self-medication during the COVID-19 infection leading to the misuse of antibiotics, especially azithromycin, as medical treatment. To summarize, anosmia, parosmia and odynophagia are prominent, persistent symptoms of COVID-19 disease in thePakistani population leading to associated mental health issues. There’s a dire need for detailed research and proper awareness campaigns through different platforms about long-covid and medical treatment to avoid further health and psychological problems.
{"title":"A PERSPECTIVE OF POST COVID-19 SYMPTOMS AND MENTAL HEALTH","authors":"None Ramna Zia, None Zahra Arshad, None Hina Batool, None Kaneez Fatima, None Syed Abdul Qadir Shah, None Muhammad Zaid","doi":"10.54692/lgujls.2023.0703278","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703278","url":null,"abstract":"The symptoms of COVID-19 are acute and mostly people recovered from these after a specific time period. Although, a considerable percentage of patients around the globe were affected but Per our knowledge, study of post covid- 19 associated mental health symptoms found scarce. The present study was organized to inspect the frequency of long-term anosmia, parosmia, odynophagia and associated mental health issues in the Pakistani population. A 5-month study was collected via an online questionnaire and phone calls among patients infected from March 2020 to February 2021. This study included a total of 124 patients with COVID-19 RT-PCR positive test. It was recorded by this survey that almost 13% of cases were with long-term anosmia, 81% with parosmia after short-term anosmia, and 79%with odynophagia. The level of depression and anxiety among patients suffering from long-term parosmia was significantly higher in participants than in anosmia. Most shockingly, a large fraction of respondents showed self-medication during the COVID-19 infection leading to the misuse of antibiotics, especially azithromycin, as medical treatment. To summarize, anosmia, parosmia and odynophagia are prominent, persistent symptoms of COVID-19 disease in thePakistani population leading to associated mental health issues. There’s a dire need for detailed research and proper awareness campaigns through different platforms about long-covid and medical treatment to avoid further health and psychological problems.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135860766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-25DOI: 10.54692/lgujls.2023.0703277
None Awais Bokhari, None Muhammad Sarwar Khan, None Muhammad Hassan Saleem, None Ruheeb Aslam Sandhu, None Ahmad Yar Qamar, None Ahmad Yar Qamar, None Muhammad Azhar, None Nazish Iqrar, None Bushra Anwar
Ancylostomiasis is a prevailing parasitic disease in cats and trend to keep cats as a pet is increasing in the society. So, the present study was designed to determine the prevalence of Ancylostomiasis in pet cats, its treatment and to see effect of Ancylostomiasis in various blood parameters. In the present study, 300 fecal samples were collected from pet cats from September 2021 to January 2022 and 65 samples (21.67%) were found positive for the Ancylostoma spp. For the chemotherapeutical trials, cats were divided into three groups viz. A, B and C. Group A was treated with Pyrantel Pamoate, Group B was treated with Albendazole. Group C was kept as a positive control. The Pyrantel Pamoate was more effective than Albendazole with an efficacy rate of 94.20% and 86.81% respectively. There was a change in hematological values due to Ancylostomiasis. The values of hemoglobin, packed cell volume, and total erythrocyte count were lower in positive samples, and the value white blood cell count was not affected much in Ancylostoma affected groups. It was concluded that Ancylostomiasis wasprevailing in cats and Pyrantel Pamoate is a good remedy to treat it as compared to Albendazole. However, disease mapping and molecular epidemiology are recommended for further detail analysis.
{"title":"Prevalence and Chemotherapy of Feline Ancylostomiasis in and around Lahore, Pakistan","authors":"None Awais Bokhari, None Muhammad Sarwar Khan, None Muhammad Hassan Saleem, None Ruheeb Aslam Sandhu, None Ahmad Yar Qamar, None Ahmad Yar Qamar, None Muhammad Azhar, None Nazish Iqrar, None Bushra Anwar","doi":"10.54692/lgujls.2023.0703277","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703277","url":null,"abstract":"Ancylostomiasis is a prevailing parasitic disease in cats and trend to keep cats as a pet is increasing in the society. So, the present study was designed to determine the prevalence of Ancylostomiasis in pet cats, its treatment and to see effect of Ancylostomiasis in various blood parameters. In the present study, 300 fecal samples were collected from pet cats from September 2021 to January 2022 and 65 samples (21.67%) were found positive for the Ancylostoma spp. For the chemotherapeutical trials, cats were divided into three groups viz. A, B and C. Group A was treated with Pyrantel Pamoate, Group B was treated with Albendazole. Group C was kept as a positive control. The Pyrantel Pamoate was more effective than Albendazole with an efficacy rate of 94.20% and 86.81% respectively. There was a change in hematological values due to Ancylostomiasis. The values of hemoglobin, packed cell volume, and total erythrocyte count were lower in positive samples, and the value white blood cell count was not affected much in Ancylostoma affected groups. It was concluded that Ancylostomiasis wasprevailing in cats and Pyrantel Pamoate is a good remedy to treat it as compared to Albendazole. However, disease mapping and molecular epidemiology are recommended for further detail analysis.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135860767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-25DOI: 10.54692/lgujls.2023.0703275
Muhammad Fayaz Khan, Aamer Ali Khattak, Afshan Saleem, Muhammad Rizwan, Muhammad Asif, Iqbal Ahmad Alvi
Pseudomonas aeruginosa biofilm infections pose significant challenges in clinical settings due to their increased resistance to conventional antibiotics. Bacteriophages, viruses that infect and kill bacteria, have emerged as promising agents for combating biofilm-related infections. This study aimed to isolate and characterize a potent bacteriophage with antibiofilm activity against P. aeruginosa. Hospital sewage was utilized to isolate a bacteriophage targeting P. aeruginosa. Quantification of phages was conducted through spot tests and doublelayer agar methods. The stability of the isolated phage was assessed under varying pH and temperature conditions. Furthermore, the bacteriophage's ability to reduce bacterial growth and exhibit antibiofilm activity was evaluated at different Multiplicity of Infection (MOI) levels. The isolated bacteriophage, named MLG, was identified as a member of the Myoviridae family within the Caudovirales order. MLG effectively reduced bacterial growth over a 14-hour period. It displayed tolerance to a pH range of 5 to 9 and temperatures spanning 25 to 60°C. Moreover, MLG demonstrated efficient inhibition of biofilm formation across various MOI levels. Given its demonstrated in vitro capacity for bacterial growth reduction and antibiofilm activity, MLG holds potential for combatting P. aeruginosa biofilm infections. This study suggests a promising avenue for the development of alternative antibiofilm strategies using bacteriophages.
{"title":"Efficient Reduction of Pseudomonas aeruginosa Biofilms Using the Myoviridae Lytic Bacteriophage vBPaeM MLG","authors":"Muhammad Fayaz Khan, Aamer Ali Khattak, Afshan Saleem, Muhammad Rizwan, Muhammad Asif, Iqbal Ahmad Alvi","doi":"10.54692/lgujls.2023.0703275","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703275","url":null,"abstract":"Pseudomonas aeruginosa biofilm infections pose significant challenges in clinical settings due to their increased resistance to conventional antibiotics. Bacteriophages, viruses that infect and kill bacteria, have emerged as promising agents for combating biofilm-related infections. This study aimed to isolate and characterize a potent bacteriophage with antibiofilm activity against P. aeruginosa. Hospital sewage was utilized to isolate a bacteriophage targeting P. aeruginosa. Quantification of phages was conducted through spot tests and doublelayer agar methods. The stability of the isolated phage was assessed under varying pH and temperature conditions. Furthermore, the bacteriophage's ability to reduce bacterial growth and exhibit antibiofilm activity was evaluated at different Multiplicity of Infection (MOI) levels. The isolated bacteriophage, named MLG, was identified as a member of the Myoviridae family within the Caudovirales order. MLG effectively reduced bacterial growth over a 14-hour period. It displayed tolerance to a pH range of 5 to 9 and temperatures spanning 25 to 60°C. Moreover, MLG demonstrated efficient inhibition of biofilm formation across various MOI levels. Given its demonstrated in vitro capacity for bacterial growth reduction and antibiofilm activity, MLG holds potential for combatting P. aeruginosa biofilm infections. This study suggests a promising avenue for the development of alternative antibiofilm strategies using bacteriophages.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135860774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-25DOI: 10.54692/lgujls.2023.0703276
None Ali Zeeshan, None Aquib Nazar, None Humaira Akram, None Irsa Hafeez, None Arshia Tariq, None Ghadir Ali, None Mohammad Zeeshan Anwar, None Muhammad Khurram, None Muhammad Haris, None Uswa Aashiq Nadeem, None Muhammad Shahzad Shah, None Anum Shafique, None Mushtaq Hussain, None Kousar Fatima, None Zarar Ahmed
Parthenium hysterophorus L, also known as false ragweed or wild carrot, is a flowering plant belonging to the Asteraceae family. It is a weed that causes many losses to ecosystem due to its ability to spread in to the agricultural and causes allergic reactions into humans and animals. This plant has many medicinal, antibacterial, antifungal, anticancerous, anti-tumor and anti-allergic activities and many folks are using it. Control measures include chemical, physical, and biological methods. The purpose of this review is to provide a comprehensive overview of the plant's origin, life cycle, geographic distribution, phytochemistry, pseudoguaianolides, flavonoids, phenolic acids, minerals, clinical applications, synthetic applications, economic importance, and control methods. This comprehensive review highlights the diverse medicinal value of P. hysterophorus and provides direction for future investigations into leveraging this plant for modern medicine.
{"title":"PARTHENIUM HYSTEROPHORUS L: A REVIEW OF ITS PHYTOCHEMICAL, BIOLOGICAL AND THERAPEUTICAL APPLICATIONS","authors":"None Ali Zeeshan, None Aquib Nazar, None Humaira Akram, None Irsa Hafeez, None Arshia Tariq, None Ghadir Ali, None Mohammad Zeeshan Anwar, None Muhammad Khurram, None Muhammad Haris, None Uswa Aashiq Nadeem, None Muhammad Shahzad Shah, None Anum Shafique, None Mushtaq Hussain, None Kousar Fatima, None Zarar Ahmed","doi":"10.54692/lgujls.2023.0703276","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703276","url":null,"abstract":"Parthenium hysterophorus L, also known as false ragweed or wild carrot, is a flowering plant belonging to the Asteraceae family. It is a weed that causes many losses to ecosystem due to its ability to spread in to the agricultural and causes allergic reactions into humans and animals. This plant has many medicinal, antibacterial, antifungal, anticancerous, anti-tumor and anti-allergic activities and many folks are using it. Control measures include chemical, physical, and biological methods. The purpose of this review is to provide a comprehensive overview of the plant's origin, life cycle, geographic distribution, phytochemistry, pseudoguaianolides, flavonoids, phenolic acids, minerals, clinical applications, synthetic applications, economic importance, and control methods. This comprehensive review highlights the diverse medicinal value of P. hysterophorus and provides direction for future investigations into leveraging this plant for modern medicine.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135860770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-25DOI: 10.54692/lgujls.2023.0703287
None Hubza Ruatt Khan, None Rabia Sultan, None Aiman shahzadi, None Mehvish Javeed, None Sara Janiad
SARS-CoV-2 has given rise to several variants such as Alpha, Beta, Gamma, Delta and Omicron are the major but not all COVID strains (variants) currently creating concern. The strains avoid antibodies response because of change in structure (mutations in genome) and thus cause disease. The most prevalent and infectious variant identified so far is Delta variant. The spike protein's structural alterations, which make it quick and fit, cause the greater occurrence. The Delta Plus variant was recently found as a variant of the Delta. Furthermore, twin variants of the Delta Plus variant are making their way around the world. Inactivated vaccines (59%), viral vectors (67.74%), and mRNA-based vaccines (77.74%) were found to be efficacious against diseases caused by the SARS-CoV-2 virus. Currently, Community immunization is an efficient strategy to stop COVID- 19; if not, it may persist for a long time and continue to wreak havoc on humanity
{"title":"DELTA AND DELTA PLUS VARIANT: A SIMPLE ENLIGHTENMENT","authors":"None Hubza Ruatt Khan, None Rabia Sultan, None Aiman shahzadi, None Mehvish Javeed, None Sara Janiad","doi":"10.54692/lgujls.2023.0703287","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703287","url":null,"abstract":"SARS-CoV-2 has given rise to several variants such as Alpha, Beta, Gamma, Delta and Omicron are the major but not all COVID strains (variants) currently creating concern. The strains avoid antibodies response because of change in structure (mutations in genome) and thus cause disease. The most prevalent and infectious variant identified so far is Delta variant. The spike protein's structural alterations, which make it quick and fit, cause the greater occurrence. The Delta Plus variant was recently found as a variant of the Delta. Furthermore, twin variants of the Delta Plus variant are making their way around the world. Inactivated vaccines (59%), viral vectors (67.74%), and mRNA-based vaccines (77.74%) were found to be efficacious against diseases caused by the SARS-CoV-2 virus. Currently, Community immunization is an efficient strategy to stop COVID- 19; if not, it may persist for a long time and continue to wreak havoc on humanity","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135859257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-25DOI: 10.54692/lgujls.2023.0703290
None Mouvez Zeeshan, None Aqeela Ashraf, None Humaira Niamat, None Muhammad Danyall Mustafa, None Laiba Riaz, None Rashid Saif
Vancomycin resistant enterococci (VRE) nosocomial diseases are rapidly spreading across the world. To treat these diseases is becoming a great challenge due to antibiotic resistance. The present study was aimed to estimate the prevalence of vancomycin resistant enterococci and to find out genes responsible for causing vancomycin resistance. One hundred different blood, wound and urine samples were collected from hospitalized patients at tertiary care health hospital, Lahore. On the basis of colony morphology, isolates of Enterococci were identified, Gram staining and biochemical tests including catalase test, Bile esculin test, growth in 6.5% NaCl, litmus milk reduction were performed. All the clinical isolates were checked for vancomycin resistant and 15% of all the samples were vancomycin resistant. Polymerase chain reaction (PCR) was performed for vancomycin resistance, enterococcal isolates to identify van genes responsible for resistance. In our study the presence of vanA gene was found in all 15 vancomycin resistant samples. vanB gene was not detected in any of the sample. The study highlights the increased VRE prevalence in clinicalisolates obtained from local hospital. The presence of vanA gene in all resistant samples is suggestive of its role as resistant gene. On the basis of these results there is an urgent need of establishing a rational antibiotic use policy for better management of enterococcal infections.
{"title":"Identification of Vancomycin Resistant Enterococcus Genes in Clinical Isolates","authors":"None Mouvez Zeeshan, None Aqeela Ashraf, None Humaira Niamat, None Muhammad Danyall Mustafa, None Laiba Riaz, None Rashid Saif","doi":"10.54692/lgujls.2023.0703290","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703290","url":null,"abstract":"Vancomycin resistant enterococci (VRE) nosocomial diseases are rapidly spreading across the world. To treat these diseases is becoming a great challenge due to antibiotic resistance. The present study was aimed to estimate the prevalence of vancomycin resistant enterococci and to find out genes responsible for causing vancomycin resistance. One hundred different blood, wound and urine samples were collected from hospitalized patients at tertiary care health hospital, Lahore. On the basis of colony morphology, isolates of Enterococci were identified, Gram staining and biochemical tests including catalase test, Bile esculin test, growth in 6.5% NaCl, litmus milk reduction were performed. All the clinical isolates were checked for vancomycin resistant and 15% of all the samples were vancomycin resistant. Polymerase chain reaction (PCR) was performed for vancomycin resistance, enterococcal isolates to identify van genes responsible for resistance. In our study the presence of vanA gene was found in all 15 vancomycin resistant samples. vanB gene was not detected in any of the sample. The study highlights the increased VRE prevalence in clinicalisolates obtained from local hospital. The presence of vanA gene in all resistant samples is suggestive of its role as resistant gene. On the basis of these results there is an urgent need of establishing a rational antibiotic use policy for better management of enterococcal infections.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135859255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}