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ESTIMATION AND DETOXIFICATION OF AFLATOXINS IN OSTRICH FEED SAMPLES COLLECTED FROM LAHORE, PAKISTAN 从巴基斯坦拉合尔收集的鸵鸟饲料样品中黄曲霉毒素的估计和解毒
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703283
None Sonal Chaudhry, None Bushra Nisar Khan, None Sajila Hina, None Waqar Ali Gill, None Ayesha Shaheen, None Romana Zulfiqar, None Madiha Ashraf
Ostrich farming is an emerging agricultural business in Pakistan with high demands for meat, eggs, oil, feathers, and leather. However, the presence of aflatoxins may lead to health hazards to poultry and consumers depending on this industry. A study for aflatoxin contamination was conducted on ostrich feed samples collected from 10 localities of Lahore with an emphasis on storage and handling conditions. The samples were analyzed using thin liquid chromatography. The aflatoxin positive feed samples were then detoxified using 0.5, 1, and 1.5 g bud powder of Moringa oleifera, incubated for 7, 14, 21, and 28 days, and were analyzed again. Results revealed maximum contamination (60%) during summers with an average temperature of 30.88 ºC and minimum (20%) in winters with an average temperature of 17.08 ºC. The major contaminant that found was B1 Aflatoxin. Results were statistically analyzed by applying Paired T–Test which proved the effectiveness of M. oleifera ratio in detoxification of feed aflatoxins. On the basis of this study, it was concluded that M. oleifera buds are an efficient biological agent against aflatoxins in feed. In addition, control of temperature and improvement in storage conditions at the sites can reduce the risks of aflatoxicosis
鸵鸟养殖在巴基斯坦是一项新兴的农业业务,对肉、蛋、油、羽毛和皮革的需求很高。然而,黄曲霉毒素的存在可能会对家禽和依赖该行业的消费者造成健康危害。对从拉合尔10个地区采集的鸵鸟饲料样本进行了黄曲霉毒素污染研究,重点研究了储存和处理条件。采用薄液相色谱法对样品进行分析。分别用0.5、1、1.5 g辣木芽粉对黄曲霉毒素阳性饲料进行解毒,孵育7、14、21、28 d,再次进行分析。结果表明,夏季污染最大(60%),平均气温为30.88ºC;冬季污染最小(20%),平均气温为17.08ºC。发现的主要污染物是B1黄曲霉毒素。采用配对t检验对结果进行统计分析,证实了油松菌比例对饲料中黄曲霉毒素的脱毒效果。在此基础上,得出油橄榄芽是一种有效的饲料黄曲霉毒素生物制剂的结论。此外,控制温度和改善储存条件可以降低黄曲霉中毒的风险
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引用次数: 0
Effect of Antimicrobial Finish on Aesthetic property of Polyester Fabric 抗菌整理剂对涤纶织物美观性能的影响
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703284
None Shama Sadaf, None Shagufta Naz, None Wajeeha Salamat, None Zeeshan Ahmad, None Saima Sharif, None Komal Hassan, None Ayesha Saeed
Antimicrobial fabric is suitable for providing protective covering to the medicinal industry, army uniform, casual wear, and household furnishing as well as for general public usage. The environmentally friendly antibacterial finish was extracted from leaves of Azadirachata indica, Butea monosperma and Litchi chinensis trees leaves and then applied on 100% polyester. Before and after applying the antimicrobial finish, fabric property was checked. The antimicrobial finish was applied by the pad dry cure method and the finish was fixed by using of polyurethane binder. Under aesthetic properties, stiffness and surface appearance were checked. The stiffness of the fabric was checked by Shirley Stiffness tester and surface appearance was checked by using AATCC Technical Manual /2004 TM 124-2001 203 test method. The aesthetic properties (smoothness, surface appearance) of the antimicrobial finish had a positive effect on 100% polyester. Results showed thatusing an antimicrobial finish made from the leaves of A. indica , B. monosperma , and L. chinensis reduced the amount of bacteria on polyester fabric by 100% when compared to the control group. The result showed that antimicrobial finish increased the stiffness while smoothness appearance was decreased in case of A. indica and B. monosperma and increased in case of L. chinensis.
抗菌面料适用于医药行业、军服、休闲服、家居用品等提供防护覆盖物,也适用于一般公众使用。环保型抗菌整理剂是从印楝、单子叶茶和荔枝树叶中提取的,然后涂在100%聚酯上。应用抗菌整理前后,检查织物性能。采用垫干固化法进行抗菌整理,并采用聚氨酯粘结剂进行整理。在美学性能下,检查了刚度和表面外观。织物的刚度采用Shirley刚度试验机进行检验,表面外观采用AATCC技术手册/2004 TM 124-2001 203试验方法进行检验。抗菌整理的美观性能(光滑度,表面外观)对100%聚酯有积极的影响。结果表明,与对照组相比,使用由籼稻、单精子双歧杆菌和羊草叶片制成的抗菌整理剂可使涤纶织物上的细菌数量减少100%。结果表明,抗菌整理剂提高了籼稻和单精子双歧杆菌的硬度,降低了光洁度,提高了羊草的光洁度。
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引用次数: 0
Evaluation of Ethical Awareness among Students to Scrutinize the Intrinsic Relationship between Humans and Animals 审视人与动物内在关系的学生伦理意识评价
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703286
None Nageen Hussain, None Mubashir Ahmad, None Abida Butt, None Amina Bashir
Awareness of ethical values between humans and animals is gaining significant attention in recent years. It is reflecting society's attitude towards animals. This survey-based research aimed to assess the ethical awareness among students and delve into the intricate web of relationships between humans and animals. The study examined the extent to which students were familiar to ethical issues related to animal welfare. This survey study designed with the students from various academic disciplines, age groups, and backgrounds. A mixed-methods approach was used to explore student’s awareness, attitudes, and experiences concerning animals. The survey reveal the multifaceted nature of ethical awareness among students and it uncovers various factors influencing students' ethical behaviors towards animals, including cultural and societal norms, personal experiences, and educational exposure. It was concluded that the survey provides valuable insights into the ethical awareness among students concerning the relationship between humans and animals. It emphasized the need for comprehensive educational programs that not only inform students about animal ethics but also encourage empathy, compassion, and responsible stewardship
近年来,人与动物之间的伦理价值意识受到了极大的关注。这反映了社会对动物的态度。这项基于调查的研究旨在评估学生的道德意识,并深入研究人类与动物之间错综复杂的关系网络。这项研究调查了学生对与动物福利有关的道德问题的熟悉程度。本研究的调查对象是来自不同学科、年龄和背景的学生。采用混合方法探讨学生对动物的认识、态度和经验。该调查揭示了学生道德意识的多面性,并揭示了影响学生对待动物道德行为的各种因素,包括文化和社会规范、个人经历和教育曝光。结论是,该调查提供了宝贵的见解,在人与动物之间的关系,学生的道德意识。它强调需要全面的教育项目,不仅要让学生了解动物伦理,还要鼓励同情、同情和负责任的管理
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引用次数: 0
EFFECT OF CAFFEINATED AND DECAFFEINATED COFFEE ON BLOOD PRESSURE 含咖啡因和不含咖啡因咖啡对血压的影响
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703281
None Tahira Khurram, None Samina Khanum, None Asma Nadeem, None Usama Kiran, None Rashida Kazmi, None Ummara Rasheed
The world's most popular stimulant, caffeine, accounts for 80% of all caffeine consumption. It is widely used as a component in pharmaceuticals due to its high antioxidant and nutrient content, including phenol and polyphenol. On the presence of caffeine drinks are categorised as caffeinated and decaffeinated. The present study was conducted to evaluate the effect of caffeinated and decaffeinated coffee on blood pressure among the young generation. This experimental study was conducted on 85 healthy individuals (both male and female, non hypertensive) between 18-28 years of age. Blood Pressure (BP) was measured by an automatic digital blood pressure monitor, before and after taking of coffee in 30, 60, and 90 minutes. Each participant ingested 100 mL of coffee prepared from 50 mg of caffeinated coffee in water. The consumption time of each individual was between 2- 4 minutes. It was noticed almost 65.7% participants were found normal while, a significant difference in 34.3% found in systolic blood pressure (SBP) and diastolic blood pressure (DBP) after the intake of caffeinated coffee at p-value (<0.001).An increase of4.4 mmHg and 5.02 mmHg was found in systolic blood pressure after 60, 90 minutes respectively from the normal that is <20mm Hg. While, a slight increase was noticed in DBP after taking caffeinated coffee. No significant difference were seen after the congestion of decaffeinated coffee among participants. It was concluded by the study that individual responses can vary. There was increase in BP after taking caffeinated coffee. There was no significant effect found after decaffeinated coffee. Some people might be more sensitive to caffeine and feel its effects more strongly, while others might not notice much difference. Additionally, any effects from this small amount of coffee are likely to wear off relatively quickly, within a few hours at most.
世界上最受欢迎的兴奋剂,咖啡因,占所有咖啡因消费的80%。由于其高抗氧化性和营养成分,包括酚和多酚,它被广泛用作药物成分。根据咖啡因的含量,饮料分为含咖啡因和不含咖啡因两种。本研究旨在评估含咖啡因和不含咖啡因的咖啡对年轻一代血压的影响。本实验研究对85名年龄在18-28岁之间的健康个体(男性和女性,无高血压)进行了研究。在饮用咖啡前、后30分钟、60分钟和90分钟,用自动数字血压监测仪测量血压(BP)。每个参与者都喝了100毫升的咖啡,这些咖啡是由50毫克含咖啡因的咖啡在水中调制而成的。每个人的消耗时间在2- 4分钟之间。值得注意的是,几乎65.7%的参与者发现正常,而在摄入含咖啡因的咖啡后,发现收缩压(SBP)和舒张压(DBP)在p值(<0.001)上有34.3%的显着差异。60分钟和90分钟后收缩压分别比正常值(20毫米汞柱)增加4.4毫米汞柱和5.02毫米汞柱,而饮用含咖啡因咖啡后舒张压略有增加。无咖啡因咖啡充血后,参与者之间没有明显差异。该研究得出的结论是,个体的反应可能会有所不同。饮用含咖啡因的咖啡后血压升高。不含咖啡因的咖啡没有明显的影响。有些人可能对咖啡因更敏感,对咖啡因的影响更强烈,而另一些人可能没有注意到太大的区别。此外,这少量咖啡产生的任何影响都可能很快消失,最多在几个小时内。
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引用次数: 0
A PERSPECTIVE OF POST COVID-19 SYMPTOMS AND MENTAL HEALTH COVID-19后症状与心理健康的视角
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703278
None Ramna Zia, None Zahra Arshad, None Hina Batool, None Kaneez Fatima, None Syed Abdul Qadir Shah, None Muhammad Zaid
The symptoms of COVID-19 are acute and mostly people recovered from these after a specific time period. Although, a considerable percentage of patients around the globe were affected but Per our knowledge, study of post covid- 19 associated mental health symptoms found scarce. The present study was organized to inspect the frequency of long-term anosmia, parosmia, odynophagia and associated mental health issues in the Pakistani population. A 5-month study was collected via an online questionnaire and phone calls among patients infected from March 2020 to February 2021. This study included a total of 124 patients with COVID-19 RT-PCR positive test. It was recorded by this survey that almost 13% of cases were with long-term anosmia, 81% with parosmia after short-term anosmia, and 79%with odynophagia. The level of depression and anxiety among patients suffering from long-term parosmia was significantly higher in participants than in anosmia. Most shockingly, a large fraction of respondents showed self-medication during the COVID-19 infection leading to the misuse of antibiotics, especially azithromycin, as medical treatment. To summarize, anosmia, parosmia and odynophagia are prominent, persistent symptoms of COVID-19 disease in thePakistani population leading to associated mental health issues. There’s a dire need for detailed research and proper awareness campaigns through different platforms about long-covid and medical treatment to avoid further health and psychological problems.
COVID-19的症状是急性的,大多数人在一段特定的时间后痊愈。尽管全球有相当大比例的患者受到影响,但据我们所知,对covid- 19后相关心理健康症状的研究发现很少。本研究旨在调查巴基斯坦人口中长期嗅觉缺失、嗅觉缺失、嗅觉缺失和相关心理健康问题的发生频率。从2020年3月到2021年2月,通过在线问卷和电话对感染患者进行了为期5个月的研究。本研究共纳入124例COVID-19 RT-PCR阳性患者。该调查记录了近13%的病例为长期嗅觉缺失,81%的病例为短期嗅觉缺失后的嗅觉缺失,79%的病例为嗅觉缺失。长期嗅觉缺失患者的抑郁和焦虑水平明显高于嗅觉缺失患者。最令人震惊的是,很大一部分受访者在COVID-19感染期间自我药疗,导致滥用抗生素,特别是阿奇霉素作为药物治疗。总之,嗅觉缺失、嗅觉缺失和嗅觉缺失是巴基斯坦人群中突出的、持续的COVID-19疾病症状,导致相关的精神健康问题。迫切需要通过不同的平台对长期covid和医疗进行详细的研究和适当的宣传,以避免进一步的健康和心理问题。
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引用次数: 0
Prevalence and Chemotherapy of Feline Ancylostomiasis in and around Lahore, Pakistan 巴基斯坦拉合尔及其周边地区猫钩虫病的流行和化疗
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703277
None Awais Bokhari, None Muhammad Sarwar Khan, None Muhammad Hassan Saleem, None Ruheeb Aslam Sandhu, None Ahmad Yar Qamar, None Ahmad Yar Qamar, None Muhammad Azhar, None Nazish Iqrar, None Bushra Anwar
Ancylostomiasis is a prevailing parasitic disease in cats and trend to keep cats as a pet is increasing in the society. So, the present study was designed to determine the prevalence of Ancylostomiasis in pet cats, its treatment and to see effect of Ancylostomiasis in various blood parameters. In the present study, 300 fecal samples were collected from pet cats from September 2021 to January 2022 and 65 samples (21.67%) were found positive for the Ancylostoma spp. For the chemotherapeutical trials, cats were divided into three groups viz. A, B and C. Group A was treated with Pyrantel Pamoate, Group B was treated with Albendazole. Group C was kept as a positive control. The Pyrantel Pamoate was more effective than Albendazole with an efficacy rate of 94.20% and 86.81% respectively. There was a change in hematological values due to Ancylostomiasis. The values of hemoglobin, packed cell volume, and total erythrocyte count were lower in positive samples, and the value white blood cell count was not affected much in Ancylostoma affected groups. It was concluded that Ancylostomiasis wasprevailing in cats and Pyrantel Pamoate is a good remedy to treat it as compared to Albendazole. However, disease mapping and molecular epidemiology are recommended for further detail analysis.
钩虫病是一种流行于猫身上的寄生虫病,社会上养猫的趋势越来越多。因此,本研究旨在确定宠物猫中钩虫病的患病率,治疗方法以及钩虫病对各种血液参数的影响。本研究于2021年9月至2022年1月收集了300份宠物猫粪便样本,其中65份(21.67%)检测出anylostoma spp阳性。在化疗试验中,将猫分为A、B、c三组,A组用吡喃吡酯治疗,B组用阿苯达唑治疗。C组作为阳性对照。吡喃吡酯比阿苯达唑更有效,有效率分别为94.20%和86.81%。有血液学值的变化,由于钩虫病。阳性标本血红蛋白值、堆积细胞体积值、红细胞总计数值较低,钩虫感染组白细胞计数值无明显影响。结论:猫钩虫病普遍存在,与阿苯达唑相比,帕莫酸吡喃酯是一种较好的治疗方法。但是,建议使用疾病作图和分子流行病学进行进一步的详细分析。
{"title":"Prevalence and Chemotherapy of Feline Ancylostomiasis in and around Lahore, Pakistan","authors":"None Awais Bokhari, None Muhammad Sarwar Khan, None Muhammad Hassan Saleem, None Ruheeb Aslam Sandhu, None Ahmad Yar Qamar, None Ahmad Yar Qamar, None Muhammad Azhar, None Nazish Iqrar, None Bushra Anwar","doi":"10.54692/lgujls.2023.0703277","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703277","url":null,"abstract":"Ancylostomiasis is a prevailing parasitic disease in cats and trend to keep cats as a pet is increasing in the society. So, the present study was designed to determine the prevalence of Ancylostomiasis in pet cats, its treatment and to see effect of Ancylostomiasis in various blood parameters. In the present study, 300 fecal samples were collected from pet cats from September 2021 to January 2022 and 65 samples (21.67%) were found positive for the Ancylostoma spp. For the chemotherapeutical trials, cats were divided into three groups viz. A, B and C. Group A was treated with Pyrantel Pamoate, Group B was treated with Albendazole. Group C was kept as a positive control. The Pyrantel Pamoate was more effective than Albendazole with an efficacy rate of 94.20% and 86.81% respectively. There was a change in hematological values due to Ancylostomiasis. The values of hemoglobin, packed cell volume, and total erythrocyte count were lower in positive samples, and the value white blood cell count was not affected much in Ancylostoma affected groups. It was concluded that Ancylostomiasis wasprevailing in cats and Pyrantel Pamoate is a good remedy to treat it as compared to Albendazole. However, disease mapping and molecular epidemiology are recommended for further detail analysis.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135860767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficient Reduction of Pseudomonas aeruginosa Biofilms Using the Myoviridae Lytic Bacteriophage vBPaeM MLG 利用肌病毒科裂解噬菌体vBPaeM MLG高效还原铜绿假单胞菌生物膜
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703275
Muhammad Fayaz Khan, Aamer Ali Khattak, Afshan Saleem, Muhammad Rizwan, Muhammad Asif, Iqbal Ahmad Alvi
Pseudomonas aeruginosa biofilm infections pose significant challenges in clinical settings due to their increased resistance to conventional antibiotics. Bacteriophages, viruses that infect and kill bacteria, have emerged as promising agents for combating biofilm-related infections. This study aimed to isolate and characterize a potent bacteriophage with antibiofilm activity against P. aeruginosa. Hospital sewage was utilized to isolate a bacteriophage targeting P. aeruginosa. Quantification of phages was conducted through spot tests and doublelayer agar methods. The stability of the isolated phage was assessed under varying pH and temperature conditions. Furthermore, the bacteriophage's ability to reduce bacterial growth and exhibit antibiofilm activity was evaluated at different Multiplicity of Infection (MOI) levels. The isolated bacteriophage, named MLG, was identified as a member of the Myoviridae family within the Caudovirales order. MLG effectively reduced bacterial growth over a 14-hour period. It displayed tolerance to a pH range of 5 to 9 and temperatures spanning 25 to 60°C. Moreover, MLG demonstrated efficient inhibition of biofilm formation across various MOI levels. Given its demonstrated in vitro capacity for bacterial growth reduction and antibiofilm activity, MLG holds potential for combatting P. aeruginosa biofilm infections. This study suggests a promising avenue for the development of alternative antibiofilm strategies using bacteriophages.
铜绿假单胞菌生物膜感染由于对常规抗生素的耐药性增加,在临床环境中构成了重大挑战。噬菌体,一种感染并杀死细菌的病毒,已经成为对抗生物膜相关感染的有希望的药物。本研究旨在分离和鉴定一种具有抗铜绿假单胞菌活性的强效噬菌体。利用医院污水分离出一种针对铜绿假单胞菌的噬菌体。通过斑点试验和双层琼脂法对噬菌体进行定量。在不同的pH和温度条件下评估分离噬菌体的稳定性。此外,在不同的感染多重性(MOI)水平下,评估了噬菌体减少细菌生长和表现出抗生物膜活性的能力。分离的噬菌体,命名为MLG,被确定为Caudovirales目肌病毒科的成员。MLG在14小时内有效地减少了细菌的生长。它对pH值范围为5至9,温度范围为25至60°C。此外,MLG在不同的MOI水平上都能有效地抑制生物膜的形成。鉴于其体外抑制细菌生长和抗生物膜活性的能力,MLG具有对抗铜绿假单胞菌生物膜感染的潜力。这项研究为利用噬菌体开发替代抗生素膜策略提供了一条有希望的途径。
{"title":"Efficient Reduction of Pseudomonas aeruginosa Biofilms Using the Myoviridae Lytic Bacteriophage vBPaeM MLG","authors":"Muhammad Fayaz Khan, Aamer Ali Khattak, Afshan Saleem, Muhammad Rizwan, Muhammad Asif, Iqbal Ahmad Alvi","doi":"10.54692/lgujls.2023.0703275","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703275","url":null,"abstract":"Pseudomonas aeruginosa biofilm infections pose significant challenges in clinical settings due to their increased resistance to conventional antibiotics. Bacteriophages, viruses that infect and kill bacteria, have emerged as promising agents for combating biofilm-related infections. This study aimed to isolate and characterize a potent bacteriophage with antibiofilm activity against P. aeruginosa. Hospital sewage was utilized to isolate a bacteriophage targeting P. aeruginosa. Quantification of phages was conducted through spot tests and doublelayer agar methods. The stability of the isolated phage was assessed under varying pH and temperature conditions. Furthermore, the bacteriophage's ability to reduce bacterial growth and exhibit antibiofilm activity was evaluated at different Multiplicity of Infection (MOI) levels. The isolated bacteriophage, named MLG, was identified as a member of the Myoviridae family within the Caudovirales order. MLG effectively reduced bacterial growth over a 14-hour period. It displayed tolerance to a pH range of 5 to 9 and temperatures spanning 25 to 60°C. Moreover, MLG demonstrated efficient inhibition of biofilm formation across various MOI levels. Given its demonstrated in vitro capacity for bacterial growth reduction and antibiofilm activity, MLG holds potential for combatting P. aeruginosa biofilm infections. This study suggests a promising avenue for the development of alternative antibiofilm strategies using bacteriophages.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135860774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PARTHENIUM HYSTEROPHORUS L: A REVIEW OF ITS PHYTOCHEMICAL, BIOLOGICAL AND THERAPEUTICAL APPLICATIONS 宫草Parthenium hysterophyus 1:其植物化学、生物学和治疗应用综述
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703276
None Ali Zeeshan, None Aquib Nazar, None Humaira Akram, None Irsa Hafeez, None Arshia Tariq, None Ghadir Ali, None Mohammad Zeeshan Anwar, None Muhammad Khurram, None Muhammad Haris, None Uswa Aashiq Nadeem, None Muhammad Shahzad Shah, None Anum Shafique, None Mushtaq Hussain, None Kousar Fatima, None Zarar Ahmed
Parthenium hysterophorus L, also known as false ragweed or wild carrot, is a flowering plant belonging to the Asteraceae family. It is a weed that causes many losses to ecosystem due to its ability to spread in to the agricultural and causes allergic reactions into humans and animals. This plant has many medicinal, antibacterial, antifungal, anticancerous, anti-tumor and anti-allergic activities and many folks are using it. Control measures include chemical, physical, and biological methods. The purpose of this review is to provide a comprehensive overview of the plant's origin, life cycle, geographic distribution, phytochemistry, pseudoguaianolides, flavonoids, phenolic acids, minerals, clinical applications, synthetic applications, economic importance, and control methods. This comprehensive review highlights the diverse medicinal value of P. hysterophorus and provides direction for future investigations into leveraging this plant for modern medicine.
Parthenium hysterophorus L,也被称为假豚草或野生胡萝卜,是一种属于菊科的开花植物。它是一种杂草,由于其在农业中的传播能力和对人类和动物的过敏反应,给生态系统造成了许多损失。这种植物具有多种药用、抗菌、抗真菌、抗癌、抗肿瘤和抗过敏活性,许多人都在使用它。控制措施包括化学、物理和生物方法。本文综述了该植物的来源、生命周期、地理分布、植物化学、假愈木酚内酯、类黄酮、酚酸、矿物质、临床应用、合成应用、经济重要性和防治方法。这篇综合综述强调了子宫草的多种药用价值,并为今后利用该植物进行现代医学研究提供了方向。
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引用次数: 0
DELTA AND DELTA PLUS VARIANT: A SIMPLE ENLIGHTENMENT 德尔塔和德尔塔加变体:一个简单的启示
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703287
None Hubza Ruatt Khan, None Rabia Sultan, None Aiman shahzadi, None Mehvish Javeed, None Sara Janiad
SARS-CoV-2 has given rise to several variants such as Alpha, Beta, Gamma, Delta and Omicron are the major but not all COVID strains (variants) currently creating concern. The strains avoid antibodies response because of change in structure (mutations in genome) and thus cause disease. The most prevalent and infectious variant identified so far is Delta variant. The spike protein's structural alterations, which make it quick and fit, cause the greater occurrence. The Delta Plus variant was recently found as a variant of the Delta. Furthermore, twin variants of the Delta Plus variant are making their way around the world. Inactivated vaccines (59%), viral vectors (67.74%), and mRNA-based vaccines (77.74%) were found to be efficacious against diseases caused by the SARS-CoV-2 virus. Currently, Community immunization is an efficient strategy to stop COVID- 19; if not, it may persist for a long time and continue to wreak havoc on humanity
SARS-CoV-2已经产生了几种变体,例如Alpha, Beta, Gamma, Delta和Omicron是主要的,但并非所有目前令人担忧的COVID菌株(变体)。由于结构的改变(基因组的突变),这些菌株避免了抗体的反应,从而导致疾病。目前发现的最普遍和最具传染性的变种是德尔塔变种。刺突蛋白的结构改变,使其快速和适应,导致更大的发生。Delta Plus变体最近被发现是Delta的变体。此外,Delta Plus变体的双变体正在世界各地制造。灭活疫苗(59%)、病毒载体(67.74%)和基于mrna的疫苗(77.74%)对SARS-CoV-2病毒引起的疾病有效。目前,社区免疫接种是遏制COVID- 19的有效战略;否则,它可能会持续很长一段时间,并继续对人类造成严重破坏
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引用次数: 0
Identification of Vancomycin Resistant Enterococcus Genes in Clinical Isolates 临床分离万古霉素耐药肠球菌基因的鉴定
Pub Date : 2023-09-25 DOI: 10.54692/lgujls.2023.0703290
None Mouvez Zeeshan, None Aqeela Ashraf, None Humaira Niamat, None Muhammad Danyall Mustafa, None Laiba Riaz, None Rashid Saif
Vancomycin resistant enterococci (VRE) nosocomial diseases are rapidly spreading across the world. To treat these diseases is becoming a great challenge due to antibiotic resistance. The present study was aimed to estimate the prevalence of vancomycin resistant enterococci and to find out genes responsible for causing vancomycin resistance. One hundred different blood, wound and urine samples were collected from hospitalized patients at tertiary care health hospital, Lahore. On the basis of colony morphology, isolates of Enterococci were identified, Gram staining and biochemical tests including catalase test, Bile esculin test, growth in 6.5% NaCl, litmus milk reduction were performed. All the clinical isolates were checked for vancomycin resistant and 15% of all the samples were vancomycin resistant. Polymerase chain reaction (PCR) was performed for vancomycin resistance, enterococcal isolates to identify van genes responsible for resistance. In our study the presence of vanA gene was found in all 15 vancomycin resistant samples. vanB gene was not detected in any of the sample. The study highlights the increased VRE prevalence in clinicalisolates obtained from local hospital. The presence of vanA gene in all resistant samples is suggestive of its role as resistant gene. On the basis of these results there is an urgent need of establishing a rational antibiotic use policy for better management of enterococcal infections.
万古霉素耐药肠球菌(VRE)院内疾病正在世界范围内迅速蔓延。由于抗生素耐药性,治疗这些疾病正成为一个巨大的挑战。本研究旨在估计万古霉素耐药肠球菌的患病率,并找出引起万古霉素耐药的基因。从拉合尔三级保健医院的住院患者收集了100份不同的血液、伤口和尿液样本。根据菌落形态鉴定分离株肠球菌,进行革兰氏染色、过氧化氢酶试验、胆汁内皮素试验、6.5% NaCl生长、石蕊乳还原等生化试验。对所有临床分离株进行万古霉素耐药检查,15%的样本对万古霉素耐药。采用聚合酶链反应(PCR)对肠球菌分离物进行万古霉素耐药鉴定。在我们的研究中,在所有15个万古霉素耐药样本中都发现了vanA基因。在所有样本中均未检测到vanB基因。该研究强调从当地医院获得的临床分离株VRE患病率增加。在所有耐药样品中均存在vanA基因,提示其作为耐药基因的作用。在这些结果的基础上,迫切需要建立合理的抗生素使用政策,以更好地管理肠球菌感染。
{"title":"Identification of Vancomycin Resistant Enterococcus Genes in Clinical Isolates","authors":"None Mouvez Zeeshan, None Aqeela Ashraf, None Humaira Niamat, None Muhammad Danyall Mustafa, None Laiba Riaz, None Rashid Saif","doi":"10.54692/lgujls.2023.0703290","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0703290","url":null,"abstract":"Vancomycin resistant enterococci (VRE) nosocomial diseases are rapidly spreading across the world. To treat these diseases is becoming a great challenge due to antibiotic resistance. The present study was aimed to estimate the prevalence of vancomycin resistant enterococci and to find out genes responsible for causing vancomycin resistance. One hundred different blood, wound and urine samples were collected from hospitalized patients at tertiary care health hospital, Lahore. On the basis of colony morphology, isolates of Enterococci were identified, Gram staining and biochemical tests including catalase test, Bile esculin test, growth in 6.5% NaCl, litmus milk reduction were performed. All the clinical isolates were checked for vancomycin resistant and 15% of all the samples were vancomycin resistant. Polymerase chain reaction (PCR) was performed for vancomycin resistance, enterococcal isolates to identify van genes responsible for resistance. In our study the presence of vanA gene was found in all 15 vancomycin resistant samples. vanB gene was not detected in any of the sample. The study highlights the increased VRE prevalence in clinicalisolates obtained from local hospital. The presence of vanA gene in all resistant samples is suggestive of its role as resistant gene. On the basis of these results there is an urgent need of establishing a rational antibiotic use policy for better management of enterococcal infections.","PeriodicalId":470988,"journal":{"name":"LGU journal of life sciences","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135859255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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LGU journal of life sciences
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