Ribosomal protein L8 regulates the expression and splicing pattern of genes associated with cancer-related pathways

IF 1.1 4区 生物学 Q3 BIOLOGY Turkish Journal of Biology Pub Date : 2023-10-18 DOI:10.55730/1300-0152.2666
LEILEI XU, GUI YANG, BIN SONG, DONG CHEN, Akbar. Yunus, JIANGTAO CHEN, XIAOGANG YANG, ZHENG TIAN
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Abstract

Background/aim: Ribosomal proteins have been shown to perform unique extraribosomal functions in cell apoptosis and other biological processes. Ribosomal protein L8 (RPL8) not only has important nonribosomal regulatory functions but also participates in the oncogenesis and development of tumors. However, the specific biological functions and pathways involved in this process are still unknown. Materials and methods: RPL8 was overexpressed (RPL8-OE) in HeLa cells. MTT assay and flow cytometry were used to detect cell proliferation and apoptosis, respectively. Transcriptome sequencing was performed to analyze the differentially expressed genes (DEGs) and regulated alternative splicing events (RASEs) by RPL8-OE, both of which were validated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay. Results: RPL8-OE inhibited cell proliferation and promoted cell apoptosis. RPL8 regulated the differential expression of many oncogenic genes and the occurrence of RASEs. Many DEGs and RASE genes (RASGs) were enriched in tumorigenesis and tumor progressionrelated pathways, including angiogenesis, inflammation, and regulation of cell proliferation. RPL8 could regulate the RASGs enriched in the negative regulation of apoptosis, consistent with its proapoptosis function. Furthermore, RPL8 may influence cancer-related DEGs by modulating the alternative splicing of transcription factors. Conclusion: RPL8 might affect the phenotypes of cancer cells by altering the transcriptome profiles, including gene expression and splicing, which provides novel insights into the biological functions of RPL8 in tumor development.
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核糖体蛋白L8调节与癌症相关途径相关基因的表达和剪接模式
背景/目的:核糖体蛋白在细胞凋亡和其他生物过程中具有独特的核糖体外功能。核糖体蛋白L8 (RPL8)不仅具有重要的非核糖体调控功能,还参与肿瘤的发生发展。然而,这一过程所涉及的具体生物学功能和途径尚不清楚。材料与方法:RPL8在HeLa细胞中过表达(RPL8- oe)。MTT法检测细胞增殖,流式细胞术检测细胞凋亡。通过转录组测序分析差异表达基因(DEGs)和RPL8-OE调控的选择性剪接事件(RASEs),并通过定量逆转录聚合酶链反应(RT-qPCR)验证。结果:RPL8-OE抑制细胞增殖,促进细胞凋亡。RPL8调控多种致癌基因的差异表达和RASEs的发生。许多deg和RASE基因(rasg)在肿瘤发生和肿瘤进展相关途径中富集,包括血管生成、炎症和细胞增殖调节。RPL8可调控富集负调控凋亡的rasg,与其促凋亡功能一致。此外,RPL8可能通过调节转录因子的选择性剪接来影响癌症相关的基因突变。结论:RPL8可能通过改变转录组谱,包括基因表达和剪接来影响癌细胞的表型,这为RPL8在肿瘤发生中的生物学功能提供了新的见解。
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
20
审稿时长
6-12 weeks
期刊介绍: The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.
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