Molecular Screening and Agronomic Trait Characterization of NLR 34449 X ISM Derived Population for their resistance against Bacterial Blight Disease

Aleena D, Padma V, Ratna Babu V`, Lal Ahamed Mohammad -, Ramana JV, Vijaya Gopal -, Sandhya Munagapati, Sundaram RM
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Abstract

Bacterial Blight (BB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major limiting factor amongst the diseases of rice in India. NLR34449 is a very popular high yielding variety. It is a popular variety not only in Andhra Pradesh but also in the neighbouring states of South India, but highly susceptible to the bacterial blight disease. A total of 802 BC1 F2 plants derived from the cross NLR 34449 and ISM were phenotypically screened for bacterial blight resistance, 687 were found to be resistant to BB. Genotyping of these plants revealed ten homozygous positive plants for all the three target BB genes viz., Xa21, xa13 and xa5. They were further assessed for key agro-morphological traits. viz., Days to 50% flowering, plant height, number of panicles per plant, thousand grain weight and grain yield per plant. Almost all the traits exhibited variation for key agronomic traits and among them, five plants were found to be performing well not only for bacterial blight resistance but also found to be superior in terms of key agronomic traits like thousand grain weight and single plant yield and flowered earlier than the parents as well. These identified plants will be advanced for further evaluation.
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NLR 34449xism衍生群体抗白叶枯病分子筛选及农艺性状分析
由米黄单胞菌引起的细菌性白叶枯病。稻瘟病(Xoo)是印度水稻病害的主要限制因素。NLR34449是一个非常受欢迎的高产品种。它不仅在安得拉邦很受欢迎,而且在印度南部邻近的邦也很受欢迎,但对细菌性枯萎病非常敏感。对NLR 34449与ISM杂交获得的802株BC1 F2进行了抗白叶枯病表型筛选,其中687株具有抗白叶枯病表型。对这些植物进行基因分型,结果显示,3个BB靶基因Xa21、xa13和xa5均有10株纯合子阳性。进一步评估了它们的关键农业形态性状。即开花天数至50%、株高、每株穗数、千粒重和每株籽粒产量。主要农艺性状几乎全部变异,其中5个品种不仅在抗白叶枯病方面表现良好,而且在千粒重、单株产量等关键农艺性状上均优于亲本,开花时间也早于亲本。这些已确定的植物将进行进一步的评价。
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