PCR- A Paradigm Shift with A Robust Quantum Jump

Abstract

Nature Communications has recognized the article titled "Engineered Helicase Replaces Thermocycler in DNA Amplification While Retaining Desired PCR Characteristics" as one of the top 25 best papers published in 2022. Gavrilov et al. (2022) engineered helicase which replaces thermocycler in DNA amplification while retaining desired PCR characteristics. No experiment was so advanced that of Gavrilov et al. LAMP (Loop-Mediated Isothermal Amplification) is limited to producing only short amplicons. SHARP (SSB-Helicase Assisted Rapid PCR) was invented in 2022. We were waiting over a year for reactions from scientific community and then employed SHARP. Gavrilov et al. showed costly PCR machine is not needed for SHARP. It has huge implication for Covid-19 research and prevention in India.