Geroprotective effect of the mitochondrial-targeted antioxidant SkQBerb in a model of chronological aging of human MB135 myoblasts

Abstract

Background. The field of gerontology is actively seeking senolytics and senomorphs that can influence the quantity and activity of senescent cells in the body. Previous studies have demonstrated the geroprotective properties of mitochondrial- directed compounds based on plastoquinone and berberine in various in vitro and in vivo models. Thus, it is plausible that SkQBerb, a mitochondrialtargeted compound combining berberine and plastoquinone, may exhibit higher geroprotective potential compared to previously studied compounds.Objective. The aim of this study is to investigate the geroprotective activity of SkQBerb in a chronological aging model using MB135 myoblasts.Materials and Methods. The effect of SkQBerb on various aging characteristics of human MB135 myoblasts was assessed. Histochemical staining for β-galactosidase was performed using a commercially available kit. The levels of proinflammatory cytokines IL-6 and IL-8 were determined using pre-made immunoassay kits. Additionally, Western blotting was employed to evaluate the levels of p21, pRb (Ser807/811), and LC3B proteins.Results. Analysis of the results revealed that SkQBerb, at concentrations ranging from 20–100 nM, effectively suppressed the accumulation of senescent cells in MB135 cells subjected to chronological aging. Furthermore, it significantly reduced the levels of IL-6 and IL-8, key factors associated with the secretory phenotype.Conclusion. The findings of this study suggest a potential geroprotective and senomorphic effect of SkQBerb. These results open up possibilities for utilizing this mitochondrial-targeted compound as a geroprotector in future applications.