Çukurova Bölgesi pamuk alanlarında Aphis gossypii Glover, 1877 (Hemiptera: Aphididae)’nin insektisit direncinin belirlenmesi

IF 0.9 4区农林科学 Q3 ENTOMOLOGY Turkiye Entomoloji Dergisi-Turkish Journal of Entomology Pub Date : 2023-10-30 DOI:10.16970/entoted.1344982

Selçuk ULUSOY, Doğancan KAHYA, Mustafa GÖKHAN, Okan ÖZGÜR

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Abstract

This study aimed to reveal resistance levels of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) populations against dimethoate, λ-cyhalothrin and chlorpyrifos-ethyl used in cotton fields in Çukurova region in Adana province in Türkiye. Populations were collected from 16 locations between 2020 and 2021. Bioassay, biochemical and molecular methods were used to determine resistance. Six populations were resistant according to the susceptible (Toktamış) population with leaf dip discriminating dose bioassays. Compared to the susceptible population, four populations were found at decreased susceptibility (DS) resistance levels to dimethoate and one population to chlorpyrifos-ethyl. . Only two populations resistance ratio were detected in MR (Moderate resistance) category to chlorpyrifos-ethyl. Resistance levels of other populations were observed as S (susceptible) category. Resistant populations had higher acetylcholinesterase, glutathione-S transferase and cytochrome P450 monooxygenase enzyme activities in biochemical analysis. The carboxylesterase gene transcription levels were higher in resistant populations. S431F and Kdr (knockdown) mutation were determined by the PCR-RLFP method, which is effective in organophosphate and pyrethroid insecticides resistance and 17% and 100% recessive alleles were detected in populations. The biochemical and mutation-induced resistance to dimethoate and chlorpyrifos-ethyl was detected. These results will contribute to developing strategies for resistance management of A. gossypii.

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确定 Çukurova 地区棉田中 Aphis gossypii Glover, 1877（半翅目：蚜科）对杀虫剂的抗性

摘要本研究旨在了解云南省阿达纳省Çukurova地区棉田1877种棉蚜(半翅目:蚜科)种群对乐果、氯氟氰菊酯和氯吡虫啉的抗性水平。在2020年至2021年期间，从16个地点收集了人口。采用生物测定法、生化法和分子法进行耐药性测定。用叶浸鉴别剂量法测定易感种群(Toktamış)，得到6个抗性种群。与易感群体相比，4个群体对乐果的敏感性降低，1个群体对毒死蜱的抗性降低。在MR(中抗性)类别中，仅检测到2个种群对毒死蜱乙酯的抗性比。其他种群抗性水平为S(易感)型。在生化分析中，抗性群体乙酰胆碱酯酶、谷胱甘肽- s转移酶和细胞色素P450单加氧酶活性较高。抗性群体羧酸酯酶基因转录水平较高。PCR-RLFP法检测到对有机磷和拟除虫菊酯杀虫剂抗性有效的S431F和Kdr突变，在群体中检测到17%和100%的隐性等位基因。对其生化抗性和突变抗性进行了检测。这些结果将有助于制定棉蚜抗性管理策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Turkiye Entomoloji Dergisi-Turkish Journal of Entomology ENTOMOLOGY-

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

>12 weeks

期刊介绍： The Turkish Journal of Entomology is a quarterly journal which has been published by Entomological Society. Turkish Journal of Entomology publishes original research articles in the fields of entomology and agricultural zoology in English. Authors whose native language is not English should have their paper edited professionally prior to submission. Before preparing the typescript for submission, examine the format of manuscripts already published in Turkish Journal of Entomology. According to TJE rules, biological observations made in one location in only one-year, short notes in which the first record of a single species in Turkey is announced, and research which is older than five years will not be accepted for publication unless it forms part of a longitudinal study.