Respon Pertumbuhan Miselium Beberapa Isolat F3 Jamur Merang (Volvariella volvaceae) FAPERTA UNSIKA pada Media PDA dengan pH yang Berbeda

Zelita Hariani, Ani Lestari, Fawzy Muhammad Bayfurqon, Winda Rianti
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Abstract

Mushroom seeds are an important factor to determine the success of mushroom cultivation. The growth of straw mushroom on PDA media is influenced by the degree of acidity (pH). This research was conducted at the Biotechnology and Plant Breeding Laboratory, Faculty of Agriculture, Singaperbangsa University, Karawang, located in Karawang Regency and start from April 2023 – May 2023. The research method used was a Completely Randomized Factorial Design. There are 2 factors consisting of 21 treatments. The first factor was the pH of PDA media which consisted of 3 levels, namely p1 (pH 6), p2 (pH 7) and p3 (pH8). Second factor was straw mushroom isolate which consisted of 7 levels, namely i1 (Parent P), i2 (Parent S), i3 (FP001), i4 (FP003), i5 (FP017), i6 (FP023) and i7 (FP024). Each treatment was repeated 3 times, resulting in 63 experimental units. The effect of the treatment was analyzed by analysis of variance at the 5% level and if it was significant to find out the best treatment it was continued with a DMRT at the 5% level. The results showed that there was an interaction effect between several F3 isolates of straw mushroom (Volvariella volvaceae) Faperta Unsika and different pH of PDA media on the diameter of mycelia colonies, mycelia growth rate and growth rate of mycelia. Treatment of PDA media with pH 8 and straw mushroom (Volvariella volvaceae) isolate Faperta Unsika FP003 gave the highest mycelial growth results with a mycelia colony diameter of 7 cm, a mycelia growth rate of 3.08 cm/day and growth rate of mycelia 2.58 cm/day.Keywords: Isolate Faperta Unsika, Mycelia, pH of PDA Media, Straw Mushroom
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菌丝对菌丝F3蘑菇飞棒(Volvariella volvaceae)菌丝在具有不同pH值的PDA中生长的反应
蘑菇种子是决定蘑菇栽培成功与否的重要因素。草菇在PDA培养基上的生长受酸度(pH)的影响。这项研究是在位于卡拉旺县的新加坡邦萨大学农业学院生物技术和植物育种实验室进行的,研究时间为2023年4月至2023年5月。研究方法为完全随机因子设计。2个因素共21个治疗方法。第一个因素是PDA培养基的pH,它由3个水平组成,即p1 (pH 6)、p2 (pH 7)和p3 (pH8)。第二个因子为草菇分离物,共7个水平,分别为i1(亲本P)、i2(亲本S)、i3 (FP001)、i4 (FP003)、i5 (FP017)、i6 (FP023)和i7 (FP024)。每次处理重复3次,共63个实验单位。通过5%水平的方差分析来分析治疗的效果,如果发现最佳治疗是显著的,则继续使用5%水平的DMRT。结果表明,不同pH的PDA培养基对草菇F3分离株菌丝菌落直径、菌丝生长速率和菌丝生长速率存在互作效应。以pH为8的PDA培养基和草菇(Volvariella volvaceae)分离物Faperta Unsika FP003处理菌丝生长效果最好,菌丝菌落直径为7 cm,菌丝生长速率为3.08 cm/d,菌丝生长速率为2.58 cm/d。毕业论文关键词:分离株,菌丝体,PDA培养基pH,草菇
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