UPLC-Q-TOF/MS analysis of chemical constituents of methanolic extract from cassava tender stems

IF 0.7 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY international food research journal Pub Date : 2023-08-30 DOI:10.47836/ifrj.30.4.09
Jie Zhang, Jianqiu Ye, Xin-Hui Xiao, Ming Wang, Kaimian Li, Jian Chen
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Abstract

In the processing and utilisation of cassava, stems and leaves usually become waste. The tender stems of cassava were analysed by electrospray ionisation high-performance liquid chromatography-tandem mass spectrometry. Using UPLC BEH C18 column (50 × 2.1 mm; i.d.: 1.7 μm), the mobile phases were H2O (0.1% FA) and CH3CN (0.1% FA), the column temperature was 30°C, and the flow rate was 0.30 mL/min. Mass spectrometry was performed by the Waters SYNAPT G2 HDMS system. This gave excellent reproducibility and avoided the interference of some similar substances. A total of 33 compounds were identified in Bzhe samples of cassava tender stems by this method, mainly cyanosides, catechins, flavonoids, glycosides, glycosides, phospholipids, and other components. The present work provided reference value for the development and utilisation of cassava tender stems.
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木薯嫩茎甲醇提取物化学成分的UPLC-Q-TOF/MS分析
在木薯的加工和利用过程中,茎和叶通常会成为废物。采用电喷雾电离高效液相色谱-串联质谱法对木薯嫩茎进行了分析。采用UPLC BEH C18色谱柱(50 × 2.1 mm;粒径:1.7 μm),流动相为H2O (0.1% FA)和CH3CN (0.1% FA),柱温30℃,流速0.30 mL/min。质谱分析采用Waters SYNAPT G2 HDMS系统。重现性好,避免了一些类似物质的干扰。通过该方法从木薯嫩茎样品中共鉴定出33种化合物,主要为氰苷、儿茶素、黄酮类化合物、糖苷、糖苷、磷脂等成分。本研究为木薯嫩茎的开发利用提供了参考价值。
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来源期刊
international food research journal
international food research journal Agricultural and Biological Sciences-Food Science
CiteScore
1.40
自引率
0.00%
发文量
75
期刊介绍: The International Food Research Journal (IFRJ) publishes papers in English, six (6) issues a year with the coverage of: Food Science and Technology Nutrition and Dietetics Agriculture, multidisciplinary Chemistry, multidisciplinary The scope of the Journal includes: Food Science, Food Technology and Food Biotechnology Product Development and Sensory Evaluation Food Habits, Nutrition, and Health Food Safety and Quality Food Chemistry, Food Microbiology, Food Analysis and Testing Food Engineering Food Packaging Food Waste Management Food Entrepreneur Food Regulatory Post-Harvest Food Management Food Supply Chain Management Halal Food and Management
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