Use of rapid immunochromatographic tests and PCR for the detection of vaccine viruses (CPV, CDV CAV) in faeces and conjunctival sac swabs of vaccinated dogs
ŁUKASZ ADASZEK, MARIA PISAREK, MARTA STANIEC, ŁUKASZ MAZUREK, ANNA KUCHARCZYK, MAŁGORZATA RUTKOWSKA-SZULCZYK, ŁUKASZ DENEKA
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Abstract
The aim of the study was to determine whether the rapid diagnostic CPV Ag (VetExpert), CDV Ag (VetExpert) and CDV/CAV Ag (VetExpert) tests are able to detect the presence of vaccine viruses in faeces and conjunctival swabs (CDV) collected from dogs vaccinated against distemper, parvovirus and Rubarth’s disease. The tested dogs were administered vaccines once, and then faeces and conjunctival sac swabs were collected from all animals on days: 2, 5, 7, 10, 14, 20, 25 after the vaccination for molecular testing and to perform rapid immunochromatographic tests for the detection of CPV, CDV and CAV antigens. The immunochromatographic tests were not able to detect the presence of any of the tested pathogens during the 25-day observation period in the vaccinated dogs, although CPV was detected in faeces by PCR up to 20 days after vaccination, and CDV and CAV up to 14 days after vaccination. Summing up the results of our own research, it should be stated that rapid immunochromatographic tests are not able to detect CPV, CDV and CAV vaccine viruses in the faeces of animals subjected to vaccination, and in the case of CDV also in the conjunctival swabs, but they are an excellent tool for detecting these pathogens in animals infected with field strains of these viruses and showing clinical symptoms of the diseases.
该研究的目的是确定快速诊断CPV Ag (VetExpert)、CDV Ag (VetExpert)和CDV/CAV Ag (VetExpert)检测是否能够检测从接种过犬瘟热、细小病毒和鲁氏病疫苗的狗收集的粪便和结膜拭子(CDV)中存在疫苗病毒。接种一次疫苗后，于接种后第2、5、7、10、14、20、25天采集所有动物的粪便和结膜囊拭子进行分子检测和快速免疫层析检测CPV、CDV和CAV抗原。免疫层析试验未能在接种犬的25天观察期内检测到任何被测病原体的存在，尽管在接种后20天通过PCR在粪便中检测到CPV，在接种后14天通过PCR在粪便中检测到CDV和CAV。总结我们自己的研究结果，需要说明的是，快速免疫层析技术不能在接种动物的粪便中检测到CPV、CDV和CAV疫苗病毒，在结膜拭子中也不能检测到CDV，但在感染这些病毒的野外菌株的动物中检测这些病原体并显示疾病的临床症状是一种很好的工具。
期刊介绍：
"Medycyna Weterynaryjna" publishes various types of articles which are grouped in the following editorial categories: reviews, original studies, scientific and professional problems, the history of veterinary medicine, posthumous memoirs, as well as chronicles that briefly relate scientific advances and developments in the veterinary profession and medicine. The most important are the first two categories, which are published with short summaries in English. Moreover, from 2001 the editors of "Medycyna Weterynaryjna", bearing in mind market demands, has also started publishing entire works in English. Since 2008 the periodical has appeared in an electronic version. The following are available in this version: summaries of studies published from 1999 to 2005, full versions of all the studies published in the years 2006-2011 (in pdf files), and full versions of the English studies published in the current year (pdf). Only summaries of the remaining studies from the current year are available. In accordance with the principles accepted by the editors, the full versions of these texts will not be made available until next year.
All articles are evaluated twice by leading Polish scientists and professionals before they are considered for publication. For years now "Medycyna Weterynaryjna" has maintained a high standard thanks to this system. The review articles are actually succinct monographs dealing with specific scientific and professional problems that are based on the most recent findings. Original works have a particular value, since they present research carried out in Polish and international scientific centers.
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