EXCEPTIONAL ANTIOXIDANT EFFECT OF URTICA DIOICA EXTRACT ON MOTILITY AND SOME OTHER POST THAWED GOAT SEMEN CHARACTERISTICS

Abstract

. This study was carried out to examine the effects of Urtica dioica extract on goat cryopreserved semen quality. The ejaculates of Markhoz goats (n = 12) were collected by an electro-ejaculator. The semen was diluted and supplemented with different concentration of aqueous or hydroalcoholic Urtica dioica extract of aerial or root parts as follows: aerial-aqueous (AA1 5μg/ml , AA2 15μg/ml and AA3 45μg/ml ); aerial-ethanolic (AE1 5μg/ml , AE2 15μg/ml and AE3 45μg/ml ); root aqueous (RA1 5μg/ml , RA2 15μg/ml and RA3 45μg/ml ); root-ethanolic (RE1 5μg/ml , RE 215μg/ml and RE3 45μg/ml ) and control (CCC 0mg/ml ) without additives. After thawing, sperm samples were subjected to viability, acrosome integrity, plasma membrane integrity, motility, sperm chromatin dispersion (SCD) and concentration of malondialdehyde (MDA) assays. Addition of AA1 5μg/ml to the extender significantly (P < 0.05) improved progressive motility, viability, plasma membrane integrity, acrosome integrity, and DNA integrity of the semen samples. Sperm viability and plasma membrane integrity in aqueous extract (both aerial & root parts)-supplemented extenders were relatively higher, as compared to the control. Moreover, a significant decrease in MDA concentration was detected in semen supplemented with Urtica dioica extract treatments. In conclusion, the results indicate that 5μg/ml of aqueous extract of Urtica dioica aerial part improved sperm quality and could be recommended as an additive to the extender for cryopreservation. This study shows a novel approach in goat semen cryopreservation due to the remarkable influence of Urtica dioica on post-thawed sperm parameters.