{"title":"BSA-SEQ-BASED METHOD FOR LOCATING KEY GENETIC SEGMENTS OF PEDUNCLE LENGTH IN BREWING DWARF SORGHUM [SORGHUM BICOLOR (L.) MOENCH]","authors":"G.D. YANG, J.H. LI, Z.Y. HU, Z.Y. HAO, B.S. SUN, C.S. LIU, Q. WANG, X.X. MENG, W. GUO","doi":"10.15666/aeer/2105_43134321","DOIUrl":null,"url":null,"abstract":". Sorghum peduncle length is one of the key agronomic traits in sorghum production and has a critical impact on sorghum mechanization for harvesting. In this study, an F2 segregating population was constructed with significantly different long-peduncle KY133B and short-peduncle KY123B as parents, and the sorghum peduncle length gene was preliminarily located using BSA-seq technology. The association analysis was performed by ED algorithm and SNP-index (or InDel-index) algorithm, and finally the sorghum peduncle length association region was targeted on chromosome 7 and 10. In this study, the BSA-seq technique was used to rapidly and efficiently locate key genetic segments of sorghum peduncle length, which laid the foundation for subsequent functional validation and molecular studies of peduncle length genes.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"35 1","pages":"0"},"PeriodicalIF":0.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Ecology and Environmental Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15666/aeer/2105_43134321","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
. Sorghum peduncle length is one of the key agronomic traits in sorghum production and has a critical impact on sorghum mechanization for harvesting. In this study, an F2 segregating population was constructed with significantly different long-peduncle KY133B and short-peduncle KY123B as parents, and the sorghum peduncle length gene was preliminarily located using BSA-seq technology. The association analysis was performed by ED algorithm and SNP-index (or InDel-index) algorithm, and finally the sorghum peduncle length association region was targeted on chromosome 7 and 10. In this study, the BSA-seq technique was used to rapidly and efficiently locate key genetic segments of sorghum peduncle length, which laid the foundation for subsequent functional validation and molecular studies of peduncle length genes.
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