Validation of Bioprinting Technique for Skin Equivalent Models

Luiza Meurer Brand, Marcelo Lazzaron Lamers, Bibiana Franzen Matte
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Abstract

Background: Skin pigmentation is a complex process; thus, skin equivalent methodologies that can reproduce the main skin structures and pigmentation have been studied. To improve the skin equivalent model, bioprinting technology has emerged, allowing for the reproduction of the complex, functional, and personalized three-dimensional architecture of the skin. Objective: Our aim was to develop a skin equivalent model and a pigmented skin equivalent model and compare the manually produced models with the bioprinted models. Methods: The study was conducted using fibroblasts, keratinocytes, and melanocytes cell lines with a 3D cell culture technique, either through bioprinting or manual production. Additionally, the bleaching potential of the model was evaluated by applying kojic acid. Results: It was observed that the bioprinted skin equivalent model demonstrated similar cell architecture and gene expression compared to the manually produced model. A pigmented skin equivalent model was developed and also bioprinted. The pigmented bioprinted skin equivalent model exhibited similar pigmentation behavior and lightening potential as the manual model. Conclusion: We have validated the use of bioprinting for reproducing skin equivalent model and cost-effective scaling of skin production.
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皮肤等效模型生物打印技术的验证
背景:皮肤色素沉着是一个复杂的过程;因此,可以重现主要皮肤结构和色素沉着的皮肤等效方法已经被研究。为了改进皮肤等效模型,生物打印技术已经出现,允许复制皮肤的复杂,功能和个性化的三维结构。目的:建立皮肤等效模型和色素皮肤等效模型,并将人工制作的模型与生物打印的模型进行比较。方法:采用生物打印或手工制作的3D细胞培养技术,使用成纤维细胞、角化细胞和黑素细胞细胞系进行研究。此外,用曲酸对模型的漂白电位进行了评价。结果:观察到生物打印的皮肤等效模型与手工制作的模型具有相似的细胞结构和基因表达。开发了色素皮肤等效模型并进行了生物打印。色素生物打印皮肤等效模型表现出与手工模型相似的色素沉着行为和浅化潜力。结论:我们已经验证了使用生物打印技术复制皮肤等效模型和具有成本效益的皮肤生产规模。
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