[Usefulness of selected enzymatic methods for coprological examination of calves].

Polskie archiwum weterynaryjne Pub Date : 1989-01-01
K Grodzki, R Lechowski, M Lenarcik
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Abstract

Methodical analysis of glutamic oxalacetic transaminase, glutamic pyruvic transaminase, alkaline phosphatase and gamma-glutamyltransferase activity in calves faeces was performed. The faeces from both healthy and diarrheic Holstein-Friesian calves, aged from 1 to 10 days, were used. For each enzyme the repetition and representativity of single determination was examined respectively in soft and dry matter. The results of our analysis show that normal enzymatic methods may be used for the determination of glutamic oxalacetic and glutamic pyruvic transaminases as well as gamma-glutamyltransferase. Further investigation methods are needed for determination of alkaline phosphatase activity in faeces.

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[选定的酶法用于犊牛的泌尿学检查的有效性]。
方法分析犊牛粪便中谷草转氨酶、谷丙转氨酶、碱性磷酸酶和γ -谷氨酰转移酶的活性。使用1 ~ 10日龄的健康和腹泻荷斯泰因-弗里西亚犊牛的粪便。对每种酶在软质物质和干质物质中分别检测了单次测定的重复性和代表性。我们的分析结果表明,正常的酶法可以用于测定谷草乙酸和谷丙转氨酶以及γ -谷氨酰转移酶。粪便中碱性磷酸酶活性的测定有待进一步的研究方法。
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