Histopathological evaluation of amino acid capped silver nanoconjugates in albino mice

Abstract

Various molecules may modify the surface chemistry of commonly used nanomaterials (NMs), resulting in the synthesis of novel and safer NMs. The current study was delineated to evaluate the in vivo toxicity profiling of the silver nanoconjugates (AgNCs) conjugated with different amino acids. The L-glycine capped-AgNCs exhibited toxicity and caused tissue damage, while L-cystine- and L-tyrosine-capped AgNCs showed protective effects against cadmium-induced toxicity. L-cystine-capped AgNCs performed well as compared to other amino-acid AgNCs. The level of serum creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase and blood urea increased (p < 0.05) in G2, G3 and G5 in comparison to G1 (control group), while an increase in bilirubin for G2 was statistically non-significant (p > 0.05). The ALT and AST elevated (p < 0.05) in G4; however, other serological parameters in G4 and G6 did not show any noticeable change in their values. Histological analysis showed disturbed and deformed cellular structures in liver and kidney tissues of G2, G3 and G5. However, G4 and G6 samples demonstrated minute changes in comparison to G1. It is concluded that L-cystine- and L-tyrosine-capped AgNCs exhibited protective effects and should be tested further for developing safer nanoconjugates for biomedical uses.