A Microwell-Based Microfluidic Device for Single-Cell Trapping and Magnetic Field Gradient Stimulation

Abstract

We develop a microfluidic platform for the long-term cultivation and observation of both THP-1 cells under different physiological conditions. First, we determine optimal seeding conditions and microwell geometry. Next, we observe changes in cell size and circularity. Results show that gradient magnetic forces on the order of 102 T/m results in stunted growth and irregular cell shapes. Finally, we observe the temporal change in ROS signals under control, static and gradient magnetic fields. For exposure to static and gradient magnetic fields, the peak in ROS signals occurs after 24 hours and 36 hours, respectively.