The target region focused imaging method for scanning ion conductance microscopy

IF 2.1 3区工程技术 Q2 MICROSCOPY Ultramicroscopy Pub Date : 2023-12-09 DOI:10.1016/j.ultramic.2023.113910

Shengbo Gu , Jian Zhuang , Tianying Wang , Shiting Hu , Weilun Song , Xiaobo Liao

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Abstract

Scanning ion conductance microscopy (SICM) has developed rapidly and has wide applications in biomedicine, single-cell science and other fields. SICM scanning speed is limited by the conventional raster-type scanning method, which spends most of time on imaging the substrate and does not focus enough on the target area. In order to solve this problem, a target region focused (TRF) method is proposed, which can effectively avoid the scanning of unnecessary substrate areas and enables SICM to image the target area only to achieve high-speed and effective local scanning. TRF method and conventional hopping mode scanning method are compared in the experiments using breast cancer cells and rat basophilic leukemia cells as experimental materials. It was demonstrated that our method can reduce the scanning time for a single sample image significantly without losing scanning information or compromising the quality of imaging. The TRF method developed in this paper can provide an efficient and fast scanning strategy for improving the imaging performance of SICM systems, which can be applied to the dynamic features of cell samples in the fields of biology and pharmacology analysis.

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扫描离子电导显微镜的靶区聚焦成像方法

扫描离子电导显微镜（SICM）发展迅速，在生物医学、单细胞科学等领域有着广泛的应用。传统的光栅式扫描方法将大部分时间花在基底成像上，对目标区域的聚焦不够，从而限制了扫描离子电导显微镜的扫描速度。为了解决这个问题，我们提出了一种目标区域聚焦（TRF）方法，它能有效避免扫描不必要的基底区域，使 SICM 只对目标区域成像，从而实现高速、有效的局部扫描。以乳腺癌细胞和大鼠嗜碱性白血病细胞为实验材料，比较了 TRF 方法和传统跳模扫描方法。结果表明，我们的方法可以在不丢失扫描信息和不影响成像质量的情况下大大缩短单个样本图像的扫描时间。本文开发的 TRF 方法可为提高 SICM 系统的成像性能提供一种高效、快速的扫描策略，可应用于生物学和药理学分析领域细胞样本的动态特征。

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来源期刊

Ultramicroscopy 工程技术-显微镜技术

CiteScore

4.60

自引率

13.60%

发文量

117

审稿时长

5.3 months

期刊介绍： Ultramicroscopy is an established journal that provides a forum for the publication of original research papers, invited reviews and rapid communications. The scope of Ultramicroscopy is to describe advances in instrumentation, methods and theory related to all modes of microscopical imaging, diffraction and spectroscopy in the life and physical sciences.