Selection for drought tolerance in backcross populations derived from interspecific crosses of Solanum lycopersicum × Solanum pennellii

IF 1.5 4区农林科学 Q2 AGRONOMY Plant Breeding Pub Date : 2023-12-11 DOI:10.1111/pbr.13154

Juliane Maciel Henschel, Diego Silva Batista, Heloisa de Souza de Voltare, André Dutra Silva Júnior, Alessandra Ferreira Ribas, Daniel Fernandes da Silva, André Ricardo Zeist

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Abstract

Drought strongly limits tomato yield, and the introgression of genes from wild tomatoes is a powerful tool to obtain drought-tolerant progenies. The aim of this study was to select drought-tolerant transgressive progeny obtained from interspecific crosses between drought-susceptible tomatoes (Solanum lycopersicum) × drought-tolerant wild species (Solanum pennellii) under in vitro and greenhouse conditions. BC₁F₂ populations were advanced from backcrosses between F₁ × Jumbo AG-592 (cultivar for fresh consumption) and F₁ × BRS Tospodoro (cultivar for industrial processing). For this, BC₁F₂ seeds were germinated in vitro and evaluated for tolerance to drought. Then, eight genotypes from each BC₁F₂ were selected and submitted to 14 days of drought (0% of water supply) or well-watered (100% of water supply) in greenhouse conditions and evaluated for growth, water balance and gas exchanges. Using in vitro assays with mannitol-induced drought proved to be effective for the initial screening of drought-tolerant BC₁F₂ plants, while greenhouse experiments showed that drought decreased photosynthesis in all genotypes, but almost all the BC₁F₂ progenies had greater photosynthetic capacity, water balance and growth than their commercial parents. As a result, we selected six progenies for fresh consumption and six progenies for industrial processing with increased drought tolerance.

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从茄科植物茄茄（Solanum lycopersicum）×茄属植物茄茄（Solanum pennellii）的种间杂交产生的回交群体中进行耐旱性选育

干旱严重限制了番茄的产量，而从野生番茄中引入基因是获得耐旱后代的有力工具。本研究的目的是在离体和温室条件下，从易旱番茄（Solanum lycopersicum）×耐旱野生种（Solanum pennellii）的种间杂交中筛选耐旱转基因后代。BC1F2 群体由 F1 × Jumbo AG-592（鲜食栽培品种）和 F1 × BRS Tospodoro（工业加工栽培品种）回交产生。为此，对 BC1F2 种子进行了离体发芽，并对其耐旱性进行了评估。然后，从 BC1F2 中各选出 8 个基因型，在温室条件下置于 14 天的干旱（0% 供水）或充足水分（100% 供水）条件下，对其生长、水分平衡和气体交换进行评估。事实证明，使用甘露醇诱导干旱的离体试验能有效地初步筛选出耐旱的 BC1F2 植物，而温室实验表明，干旱会降低所有基因型的光合作用，但几乎所有 BC1F2 亲本的光合能力、水分平衡和生长都高于其商品亲本。因此，我们选择了 6 个用于新鲜食用的后代和 6 个用于工业加工的后代，它们的抗旱能力都有所提高。

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来源期刊

Plant Breeding 农林科学-农艺学

CiteScore

4.40

自引率

5.00%

发文量

审稿时长

3.0 months

期刊介绍： PLANT BREEDING publishes full-length original manuscripts and review articles on all aspects of plant improvement, breeding methodologies, and genetics to include qualitative and quantitative inheritance and genomics of major crop species. PLANT BREEDING provides readers with cutting-edge information on use of molecular techniques and genomics as they relate to improving gain from selection. Since its subject matter embraces all aspects of crop improvement, its content is sought after by both industry and academia. Fields of interest: Genetics of cultivated plants as well as research in practical plant breeding.