Characterization and Genetic Mapping of Resistance to Cotton–Melon Aphid (Aphis gossypii) in Cucumber

IF 1.5 4区 农林科学 Q2 AGRONOMY Plant Breeding Pub Date : 2024-08-30 DOI:10.1111/pbr.13213
Shixiong Ning, Lei Xia, Yu Fang, Zhengyue Zhou, Yuhui Wang, Jinfeng Chen
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Abstract

Cotton–melon aphid (Aphis gossypii) is a highly destructive pest that causes serious yield losses in cucumber production. IL52, a Cucumis hystrix introgression line of cucumber, exhibits resistance to a number of diseases and also shows strong resistance to aphids. To characterize the type of aphid resistance in IL52, we designed a separate leaf‐disc/no‐choice test and free‐choice test to examine antibiosis and antixenosis, respectively. Our results indicated that IL52 displayed antixenosis resistance to aphids. In other words, when planted with other lines such as CCMC, IL52 was not favourable to aphids. To dissect the genetic basis of antixenosis resistance in IL52, we evaluated a mapping population of 155 lines from CCMC × IL52–derived F7:8 RIL using free‐choice test for aphid resistance. A major‐effect QTL on Chr2, designated as qRag2.1 (Resistant to A. gossypii), was identified as being significantly associated with aphid resistance. The QTL qRag2.1 was mapped to an interval flanked by markers UW085197 and SSR11909, spanning a physical location of 15.14–19.07 Mb, which explained 12.74%–14.03% of the phenotypic variance. Analysis of polymorphisms between the parental lines using resequencing data revealed one gene, CsaV3_2G018180, with three nonsynonymous amino acid substitutions and a 6‐bp InDel in its coding sequence (CDS). CsaV3_2G018180 encodes a pectinesterase/pectinesterase inhibitor enzyme, designated as CsPEI, which has been reported to regulate pectin methylesterase activity that is implicated in various biological processes. The relative expression level of CsPEI was induced in IL52 post aphid infestation, suggesting it could be a potential candidate gene for aphid resistance.
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黄瓜抗棉瓜蚜特性鉴定与遗传图谱绘制
棉瓜蚜(Aphis gossypii)是一种破坏性很强的害虫,会给黄瓜生产造成严重的产量损失。IL52 是黄瓜的一个 Cucumis hystrix 引种品系,对多种病害具有抗性,对蚜虫也有很强的抗性。为了鉴定 IL52 的抗蚜性类型,我们设计了单独的叶盘/无选择试验和自由选择试验,分别考察其抗菌性和抗蚜性。结果表明,IL52 对蚜虫表现出抗蚜性。换句话说,当与其他品系(如 CCMC)一起种植时,IL52 对蚜虫不利。为了剖析IL52抗蚜虫的遗传基础,我们采用自由选择测试法对CCMC × IL52衍生的F7:8 RIL的155个品系进行了抗蚜性测绘。结果发现 Chr2 上的一个主要效应 QTL qRag2.1(抗棉蚜)与蚜虫抗性显著相关。QTL qRag2.1 被映射到标记 UW085197 和 SSR11909 侧翼的区间,物理位置跨度为 15.14-19.07 Mb,解释了 12.74%-14.03% 的表型变异。利用重测序数据分析亲本品系间的多态性,发现一个基因 CsaV3_2G018180,其编码序列(CDS)中有三个非同义氨基酸替换和一个 6-bp 的 InDel。CsaV3_2G018180 编码一种果胶酯酶/pectinesterase 抑制剂酶,被命名为 CsPEI,据报道它能调节果胶甲基酯酶的活性,而果胶甲基酯酶与多种生物过程有关。蚜虫侵染后,CsPEI 的相对表达水平在 IL52 中被诱导,这表明它可能是抗蚜虫的潜在候选基因。
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来源期刊
Plant Breeding
Plant Breeding 农林科学-农艺学
CiteScore
4.40
自引率
5.00%
发文量
74
审稿时长
3.0 months
期刊介绍: PLANT BREEDING publishes full-length original manuscripts and review articles on all aspects of plant improvement, breeding methodologies, and genetics to include qualitative and quantitative inheritance and genomics of major crop species. PLANT BREEDING provides readers with cutting-edge information on use of molecular techniques and genomics as they relate to improving gain from selection. Since its subject matter embraces all aspects of crop improvement, its content is sought after by both industry and academia. Fields of interest: Genetics of cultivated plants as well as research in practical plant breeding.
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