Validation of the analysis method for marker compound ellagic acid of ethanol extracts of Sanguisorba officinalis L. using HPLC-DAD

Q2 Agricultural and Biological Sciences Food Science and Technology Pub Date : 2023-12-05 DOI:10.5327/fst.00020

Soon-Ho Yim, Seok-Jun Kang, H. Seon, Na-Young Byun

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Abstract

The validation of ellagic acid as a marker compound was attempted as an analysis method for the standardization of the extract from Sanguisorba officinalis L. for the development of healthy functional foods. The ellagic acid analysis using high-performance liquid chromatography with diode-array detection (HPLC-DAD) and retention time with standard compounds satisfied the stay time and specificity. Ellagic acid has confirmed limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery rate, stability, and content analysis by HPLC for an 80% ethanol extract of Sanguisorba officinalis L. The correlation (R2) at the concentration of ellagic acid of 9.5–95.0 µg/mL was above 0.999, showing high linearity. The LOD and LOQ were expressed as 1.5430 and 4.6757 μg/mL, respectively. The recovery rate was 99 to 110%. The precision of RSD intraday was 0.23–0.65% and interday was 0.18–2.13%. In addition, the average recovery rate of ellagic acid before and after 24 h was 98.79%, confirming a change in the content of 0.17%, and the content of ellagic acid was 22.48 µg/mL in 80% ethanol extract of Sanguisorba officinalis L.

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利用 HPLC-DAD 验证山银花乙醇提取物中标记化合物鞣花酸的分析方法

试图将鞣花酸作为标志化合物进行验证，并将其作为标准分析方法，为开发健康功能食品提供依据。采用高效液相色谱二极管阵列检测(HPLC-DAD)对鞣花酸进行分析，保留时间满足标准化合物的停留时间和特异性。鞣花酸对八成乙醇提取液的检出限(LOD)、定量限(LOQ)、准确度、精密度、回收率、稳定性和含量进行了HPLC分析，在鞣花酸浓度9.5 ~ 95.0µg/mL范围内，相关系数(R2)均在0.999以上，线性关系良好。定量限和定量限分别为1.5430和4.6757 μg/mL。回收率为99 ~ 110%。日内RSD精密度为0.23 ~ 0.65%，日内RSD精密度为0.18 ~ 2.13%。此外，鞣花酸在提取前后24 h的平均回收率为98.79%，含量变化0.17%，80%乙醇提取液中鞣花酸的含量为22.48µg/mL。

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