{"title":"Development of Aged Skin Equivalent Model","authors":"L. M. Brand, M. Lamers, Bibiana Franzen","doi":"10.2174/0126667797269907231118130449","DOIUrl":null,"url":null,"abstract":"\n\nSkin aging is a result of the aging process and also intrinsic and extrinsic\nfactors. In order to better understand this process and evaluate anti-aging products, it is necessary\nto develop in vitro models that can recapitulate the biological process of aged skin. Therefore, the\naim of this study was to develop an aged skin equivalent model to evaluate these properties.\n\n\n\nHuman fibroblasts were incorporated into the collagen matrix and keratinocytes were\nadded and cultured in an air-liquid interface for 21 days. During this period, the matrices were\nexposed to UV or EX527 to trigger biological aging processes.\n\n\n\nThe two protocols evaluated demonstrated reduced expression of genes related to longevity and regulation of cellular redox homeostasis, and it was confirmed with histological analysis. Also, the model demonstrated the anti-aging potential of resveratrol.\n\n\n\nThe model developed is a promising platform for reproducing aged skin and evaluating rejuvenating agents.\n","PeriodicalId":10817,"journal":{"name":"Current Cosmetic Science","volume":"68 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Cosmetic Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/0126667797269907231118130449","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Skin aging is a result of the aging process and also intrinsic and extrinsic
factors. In order to better understand this process and evaluate anti-aging products, it is necessary
to develop in vitro models that can recapitulate the biological process of aged skin. Therefore, the
aim of this study was to develop an aged skin equivalent model to evaluate these properties.
Human fibroblasts were incorporated into the collagen matrix and keratinocytes were
added and cultured in an air-liquid interface for 21 days. During this period, the matrices were
exposed to UV or EX527 to trigger biological aging processes.
The two protocols evaluated demonstrated reduced expression of genes related to longevity and regulation of cellular redox homeostasis, and it was confirmed with histological analysis. Also, the model demonstrated the anti-aging potential of resveratrol.
The model developed is a promising platform for reproducing aged skin and evaluating rejuvenating agents.
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