Neochloris oleoabundans cell wall rupture through melittin peptide: a new approach to increase lipid recovery

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2023-12-18 DOI:10.1007/s10529-023-03451-2

Magda Vargas-Pérez, Azucena González-Horta, Hiram Mendoza-Hernández, Myriam Elías-Santos, Karim Acuña-Askar, Luis Jesús Galán-Wong, Hugo Alberto Luna-Olvera

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Abstract

Objectives

Microalgae cell wall affects the recovery of lipids, representing one of the main difficulties in the development of biofuel production. This work aimed to test a new method based on melittin peptide to induce a cellular disruption in N. oleoabundans.

Results

Neochloris oleoabundans cells were grown at 32 °C in the presence of a high concentration of nitrate-phosphate, causing a cell disruption extent of 83.6%. Further, a two-fold increase in lipid recovery following melittin treatment and solvent extraction was observed. Additionally, it was possible to verify the effects of melittin, both before and after treatment on the morphology of the cells. Scanning electron microscopy (SEM) and confocal images of the melittin-treated microalgae revealed extensive cell damage with degradation of the cell wall and release of intracellular material.

Conclusions

Melittin produced a selective cell wall rupture effect in N. oleoabundans under some culture conditions. These results represent the first report on the effect of melittin on lipid recovery from microalgae.

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通过美利汀肽使油菜新孢子虫细胞壁破裂：提高脂质回收率的新方法

目的微藻类细胞壁会影响脂质的回收，这是开发生物燃料生产的主要困难之一。本研究旨在测试一种基于美利汀肽的新方法，以诱导油绿微囊藻细胞破壁。结果油绿微囊藻细胞在 32 ℃、高浓度硝酸盐-磷酸盐存在下生长，细胞破壁率达 83.6%。此外，经美乐汀处理和溶剂萃取后，观察到脂质回收率提高了两倍。此外，还验证了美乐汀处理前后对细胞形态的影响。经美乐汀处理的微藻的扫描电子显微镜（SEM）和共聚焦图像显示了广泛的细胞损伤，细胞壁降解，细胞内物质释放。结论在某些培养条件下，美利汀能使油菜褐藻产生选择性细胞壁破裂效应。这些结果是有关美乐汀对微藻类脂质回收影响的首次报道。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.