Impact of thermal desorption tubes on the variability of exhaled breath data.

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of breath research Pub Date : 2023-12-22 DOI:10.1088/1752-7163/ad15a3
Anne E Jung, Christina N Davidson, Christopher J Land, Aubrianne I Dash, Barlow T Guess, Heidi S Edmonds, Rhonda L Pitsch, Sean W Harshman
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Abstract

Due to the overall low abundance of volatile compounds in exhaled breath, it is necessary to preconcentrate the sample prior to traditional thermal desorption (TD) gas chromatography mass spectrometry analysis. While certain aspects of TD tubes, such as volatile storage, have been evaluated, many aspects remain uncharacterized. Two common TD tubes, Tenax TA and Biomonitoring 5TD tubes, were evaluated for background content and flow rate variability. The data illustrate that the Biomonitoring 5TD tubes have the highest number (23) and abundance of background contamination greater than 3x the mean noise when compared to Tenax TA (13) and empty tubes (9). Tentative identifications of the compounds in the background contamination experiment show that greater than 59% (16/27) of the compounds identified have been reported in the breath literature. The data illustrate the TD tube background abundance could account for more than 70% of the chromatographic signal from exhaled breath for these select compounds. Flow rate measurements of 200 Tenax TA and 200 Biomonitoring 5TD tubes show a large range in measured flow rates among the TD tubes (Tenax: 252.9-284.0 ml min-1, 5TD: 220.6-255.1 ml min-1). Finally, TD tubes of each type, Tenax TA and Biomonitoring 5TD, previously established to have high, medium, and low flow rates, show insignificant differences (p> 0.05) among the tubes of different flow rates, using both gas standards and an exhaled breath from a peppermint experiment. Collectively, these results establish overall background compounds attributed to each TD tube type tested. Additionally, while measured flow rate variability is present and plausibly impacts exhaled breath results, the data demonstrate no statistically significant difference was observed between tubes showing high, medium, and low flow rates from two separate sample types.

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热解吸管对呼气数据变异性的影响。
由于呼出气体中挥发性化合物的总体丰度较低,因此在进行传统的热脱附气相色谱质谱分析(TD-GC-MS)之前,有必要对样品进行预浓缩。虽然已经对热解吸附管的某些方面(如挥发物储存)进行了评估,但许多方面仍未定性。我们对 Tenax TA 和 Biomonitoring 5TD 这两种常见的热解吸附管进行了背景含量和流速变化的评估。数据表明,与 Tenax TA 管(13 个)和空管(9 个)相比,Biomonitoring 5TD 管的本底污染数量最多(23 个),丰度超过平均噪声的 3 倍。背景污染实验中化合物的初步鉴定结果表明,59%以上(16/27)的已鉴定化合物在呼吸文献中已有报道。这些数据表明,TD 管的背景丰度可能占呼气中这些特定化合物色谱信号的 70% 以上。对 200 支 Tenax TA 和 200 支 Biomonitoring 5TD 管进行的流速测量显示,TD 管的流速测量值范围很大(Tenax:252.9-284.0mL min-1,5TD:220.6-255.1mL min-1)。最后,Tenax TA 和 Biomonitoring 5TD 这两种类型的 TD 管以前分别被确定为具有高、中和低流速,在使用气体标准和薄荷实验呼出的气体时,不同流速的 TD 管之间的差异不显著(p>0.05)。总之,这些结果确定了每种测试的 TD 管类型的总体背景化合物。此外,虽然测得的流速存在变化,并可能对呼气结果产生影响,但数据表明,从两种不同的样品类型中观察到的高、中、低流速试管之间没有统计学意义上的显著差异。
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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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