{"title":"Tetrahydrobiopterin inhibits copper-induced oxidation of low density lipoprotein.","authors":"S Sakuma, Y Fujimoto, Y Gohda, T Fujita","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The effect of tetrahydrobiopterin (BPH4) on Cu2+-induced oxidation of low density lipoprotein (LDL) prepared from rabbit plasma was examined. BPH4 (0.5-10 microM) inhibited the formation of thiobarbituric acid-reactive substances (TBARS) in LDL induced by 5 microM Cu2+ in a dose-dependent manner. BPH4 also suppressed the increase in relative electrophoretic mobility (REM) of LDL in the presence of 5 microM Cu2+. The potency of inhibitions of BPH4 on TBARS formation and REM was the same as or stronger than that of ascorbic acid and alpha-tocopherol, which are known as endogenous inhibitors of LDL oxidation. These results suggest that BPH4 could act as an efficacious endogenous inhibitor of atherogenic LDL modification.</p>","PeriodicalId":502122,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"107 5-6","pages":"397-406"},"PeriodicalIF":0.0000,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research communications in molecular pathology and pharmacology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The effect of tetrahydrobiopterin (BPH4) on Cu2+-induced oxidation of low density lipoprotein (LDL) prepared from rabbit plasma was examined. BPH4 (0.5-10 microM) inhibited the formation of thiobarbituric acid-reactive substances (TBARS) in LDL induced by 5 microM Cu2+ in a dose-dependent manner. BPH4 also suppressed the increase in relative electrophoretic mobility (REM) of LDL in the presence of 5 microM Cu2+. The potency of inhibitions of BPH4 on TBARS formation and REM was the same as or stronger than that of ascorbic acid and alpha-tocopherol, which are known as endogenous inhibitors of LDL oxidation. These results suggest that BPH4 could act as an efficacious endogenous inhibitor of atherogenic LDL modification.
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