Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans

IF 4.5 3区医学 Q1 MICROBIOLOGY International Journal of Medical Microbiology Pub Date : 2023-12-19 DOI:10.1016/j.ijmm.2023.151596

Qiulei Zhang , Jingxiao Zhang , Yu Zhang , Yujie Sui , Yang Du , Longfei Yang , Yongjie Yin

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Abstract

The opportunistic fungal pathogen Candida albicans could cause severe clinical outcomes which could be exacerbated by the scarcity of antifungals. The capacity of C. albicans to form biofilms on medical devices that are hard to eradicate, further deepen the need to develop antifungal agents. In this study, we, for the first time, showed that patchouli alcohol (PA) can inhibit the growth of multiple C. albicans strains, as well as four other Candida species, with MICs of 64 μg/mL and MFCs from 64 to 128 μg/mL. The biofilm formation and development, adhesion, yeast-to-hyphal transition and extracellular polysaccharide of C. albicans can be inhibited by PA in a concentration-dependent manner. Confocal microscopy analyses of cells treated with PA showed that PA can increase the membrane permeability and intracellular reactive oxygen species (ROS) production. In C. elegans, PA did not influence the survival below 64 μg/mL. In this study PA demonstrated antifungal and antibiofilm activity against C. albicans and our results showed the potential of developing PA to fight Candida infections.

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广藿香醇对白色念珠菌的抗真菌和抗生物膜活性。

机会性真菌病原体白色念珠菌会导致严重的临床后果，而抗真菌药物的匮乏又会加剧这种后果。白念珠菌能在医疗器械上形成难以根除的生物膜，这进一步加深了开发抗真菌药物的必要性。在这项研究中，我们首次发现广藿香醇（PA）能抑制多种白念珠菌菌株以及其他四种念珠菌的生长，其 MICs 为 64 μg/mL，MFCs 为 64 至 128 μg/mL。PA 能以浓度依赖性的方式抑制白念珠菌生物膜的形成和发展、粘附、酵母到蘑菇的转化以及胞外多糖。共聚焦显微镜分析表明，PA 能增加细胞膜的通透性和细胞内活性氧（ROS）的产生。在 elegans 中，低于 64 μg/mL 的 PA 不会影响其存活率。在这项研究中，PA 对白念珠菌具有抗真菌和抗生物膜的活性，我们的研究结果表明了开发 PA 来抗击念珠菌感染的潜力。

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来源期刊

International Journal of Medical Microbiology 医学-病毒学

CiteScore

9.70

自引率

0.00%

发文量

审稿时长

45 days

期刊介绍： Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.