The effect of modern hepatotoxicants on the elimination of caffeine

Abstract

Hepatotoxicants are a variety of chemical substances that can have organ-specific damaging  effects,  which  may  be  the  result  of their direct effects on both hepatocytes and other structural units of the liver. The purpose of this study is to evaluate the effect of modern hepatotoxicants on caffeine elimination. The studies were carried out in the vivarium of the Department of Pharmacology and Toxicology of the Federal State Budgetary Educational Institution of Higher Education  of  St.  Petersburg  State  University  of Medicine. Industrial substances of different chemical  groups  have  been  used  to  induce hepatotoxic  conditions:  phthalic  anhydride, ethylenediamine and chloroform. All of the above  toxicants  were  given  in  dosages  for the immediate (within 24 hours) induction of hepatotoxic  conditions.  One  day  after  the completion  of  the  simulation,  the  levels  of caffeine  in  the  blood  serum  of  the  experimental animals were determined in full accordance with the previous series of experiments, using a dosage of 100 mg/kg, since with this dosage regimen  we identified  the most stable pharmacokinetic profiles of caffeine.  Mathematical  and  statistical  processing of the obtained data was carried out using  the  STATISTICA  6.1  program.  The arithmetic mean (M) and its mean error (m) were calculated; the significance of the difference (p) was not calculated using the Student's test due to the  exploratory nature of the study and the lack of comparison groups. Based  on  the  conducted  research,  we  can conclude that each hepatotoxicant has individual  characteristic  characteristics,  which allows us to recommend a caffeine test for different types of hepatotoxicity, incl. unexpressed. That is, in this way it is possible to diagnose  hepatotoxic  conditions  even  in  a latent form, which can be used as a method for  assessing  the  toxicity  of  a  drug  at  the stage of preclinical development.