Rajaa Umran, Abbas Mol, Amal Umran Mosa, Murat Kaya
{"title":"Characterization of intact chitosan obtained from Bradyporus sp. (Orthoptera, Insecta) and its cytotoxicity on human cell line (HEK293)","authors":"Rajaa Umran, Abbas Mol, Amal Umran Mosa, Murat Kaya","doi":"10.29002/asujse.1382667","DOIUrl":null,"url":null,"abstract":"Intact chitosan film was produced from Bradyporus sp. (Orthoptera, Insecta) and \ncharacterized by Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric \nAnalysis (TGA), X-ray diffractometer (XRD), Scanning Electron Microscopy (SEM) and \nelemental analysis. MTT test was performed to determine the cytotoxic effects of chitosan. \nChitosan administered at optimal and overdose intervals was applied to HEK293 cells for 24, \n48 and 72 hours. In the MTT cytotoxicity test, the IC 50 dose of chitosan was 1427 µM at 24 \nhours, 808.9 µM at 48 hours, and 611.6 µM at 72 hours. Considering all the conducted \nanalyses and cytotoxic experiments, the obtained intact novel chitosan film from the insects \ncould find applications for biotechnological applications.","PeriodicalId":7626,"journal":{"name":"Aksaray University Journal of Science and Engineering","volume":"2 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aksaray University Journal of Science and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29002/asujse.1382667","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Intact chitosan film was produced from Bradyporus sp. (Orthoptera, Insecta) and
characterized by Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric
Analysis (TGA), X-ray diffractometer (XRD), Scanning Electron Microscopy (SEM) and
elemental analysis. MTT test was performed to determine the cytotoxic effects of chitosan.
Chitosan administered at optimal and overdose intervals was applied to HEK293 cells for 24,
48 and 72 hours. In the MTT cytotoxicity test, the IC 50 dose of chitosan was 1427 µM at 24
hours, 808.9 µM at 48 hours, and 611.6 µM at 72 hours. Considering all the conducted
analyses and cytotoxic experiments, the obtained intact novel chitosan film from the insects
could find applications for biotechnological applications.
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