An immunochromatographic test for the detection of anti-tuberculosis antibodies

Abstract

Tuberculosis is a dangerous socially significant  disease  of  various  animal  species. According  to  Rosselkhoznadzor  data  for 2022, the situation with bovine tuberculosis is endemic, stable, long-term trends are decreasing,  epidemic  thresholds  for  ill  health and  incidence  have  not  been  overcome.  It should be noted that in 2022, bovine tuberculosis was not officially detected, but this infection was registered in several pigs and wild boars. Take a point that the reduction in the number of cases of tuberculosis in cattle is decreased (to zero), the overall strategy for combating this disease is successful, but this does not mean that the need to develop new tests  for  the  accelerated  diagnosis  of  this infection  has  completely  disappeared.  The safety of service personnel directly depends on  minimizing  the  risk  of  infection  with pathogens common to humans and animals, one of which is tuberculosis. The fastest way to indicate infectious agents is by immunochromatographic  analysis.  This  publication is devoted to the details that it is desirable to focus on when developing a diagnostic test based on immunochromatographic analysis, using the example of bovine tuberculosis. As an antigen in the presented study, native antigens of Mycobacterium bovis grown on a nutrient  medium  Levenshtein-Jensen  with proven antigenic activity were used. Conjugation of antispecies antibodies was carried out  with  a  colloidal  solution  of  gold, (diameter  of  particle  is  25±0,9  nm).  In  the course  of  the  work,  the  optimal  conditions for the manufacture of a diagnostic test for the detection of anti-tuberculosis antibodies by  immunochromatographic  analysis  were shown.