Informativeness of Blood L-Lactate and Integral Leukocyte Indices for Prediction of Severe Mycoplasmosis in Calves

Russian Agricultural Sciences Pub Date : 2023-12-26 DOI:10.3103/s1068367423050038

A. E. Chernitskiy, I. A. Shkuratova, A. P. Poryvaeva, E. V. Pechura, O. G. Tomskikh

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Abstract

The study was carried out to assess the informativeness of blood L-lactate and integral leukocyte indices to predict severe mycoplasmosis in calves naturally infected with M. bovis. In the conditions of agricultural enterprises of the Ural Federal District, daily clinical observation of 36 Holstein calves naturally infected with M. bovis at the age of 10–15 days was carried out for 1 month. In blood samples obtained from the jugular vein of animals at the first signs of the disease, the content of hemoglobin, erythrocytes, leukocytes, platelets, and also hematocrit was determined on an Abacus Junior Vet analyzer (Diatron), the concentration of L-lactate was determined by reaction with paraoxy-diphenyl on a UV-1800 spectrophotometer (Shimadzu), a differential count of leukocytes in blood smears stained according to Romanovsky-Giemsa was performed on an Olympus BX43 microscope (Olympus, Japan), and the integral leukocyte indices, the Krebs’ index (KI), the leukocyte intoxication index of Ya.Ya. Kalf-Kalif (LII), reactive neutrophil response by T.Sh. Khabirov (RNR), adaptation index (AI), Bredeck’s index (BI), nuclear shift index (NSI), lymphocyte-granulocyte index (LGI), neutrophil-monocyte ratio index (NMR), index of blood leukocyte shift (BLSI), lymphocyte-monocyte ratio index (LMR), and lymphocyte-neutrophil ratio (LNR) were also calculated. A mild and moderate course of mycoplasmosis was established in 22 (61.1%) calves and a severe course in 14 (38.9%). In animals predisposed to a severe course of the disease, an increased level of L-lactate in the blood was recorded at the first clinical signs of the disease: > 1.68 mmol/l, AI > 1.57 units, LGI > 16.7 units, LNR > 1.41 units, reduced KI < 0.645 units, and BLSI < 0.600 units. The informativeness of predictors for prognosticating severe mycoplasmosis in calves was assessed as very good, sensitivity was 71.4–85.7% and specificity was 81.8–90.9%. The accumulation of L-lactate in the blood of animals and changes in leukocyte indices (AI, LGI, LNR, KI, BLSI) are interrelated.

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预测犊牛严重支原体病的血液 L-乳酸盐和白细胞指数的信息量

摘要本研究旨在评估血液 L-乳酸盐和白细胞整体指数在预测自然感染牛霉形体病犊牛的严重霉形体病方面的信息量。在乌拉尔联邦区农业企业的条件下，对 36 头 10-15 日龄自然感染牛霉形体的荷斯坦犊牛进行了为期 1 个月的日常临床观察。从动物颈静脉采集的血液样本中，在出现疾病初期症状时，用 Abacus Junior Vet 分析仪（Diatron）测定血红蛋白、红细胞、白细胞、血小板和血细胞比容的含量，用 UV-1800 分光光度计（Shimadzu）通过与对氧二苯基反应测定 L-乳酸盐的浓度、在奥林巴斯 BX43 显微镜（奥林巴斯，日本）上对根据 Romanovsky-Giemsa 染色的血液涂片中的白细胞进行差分计数，并对 Ya.Ya.Kalf-Kalif的白细胞中毒指数（LII）、反应性中性粒细胞反应（T.Sh.Khabirov的反应性中性粒细胞反应（RNR）、适应指数（AI）、布雷德克指数（BI）、核转移指数（NSI）、淋巴细胞-粒细胞指数（LGI）、中性粒细胞-单核细胞比率指数（NMR）、血白细胞转移指数（BLSI）、淋巴细胞-单核细胞比率指数（LMR）和淋巴细胞-中性粒细胞比率（LNR）。22头（61.1%）犊牛的支原体病程为轻度和中度，14头（38.9%）犊牛的支原体病程为重度。在易患重度病程的动物中，在出现最初临床症状时，血液中的左旋乳酸水平升高：1.68毫摩尔/升、AI 1.57单位、LGI 16.7单位、LNR 1.41单位、KI 0.645单位和BLSI 0.600单位。犊牛严重支原体病预后预测指标的信息灵敏度为71.4%-85.7%，特异性为81.8%-90.9%。动物血液中 L-乳酸盐的积累与白细胞指数（AI、LGI、LNR、KI、BLSI）的变化相互关联。

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Russian Agricultural Sciences

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