{"title":"Isolation and characterization of a bisphenol A-degrading strain, <i>Pseudomonas aeruginosa</i> DU2, from soil containing decaying plants.","authors":"Navid Chamak, Parisa Farrokh, Roohollah Rostami, Fatemeh Salimi","doi":"10.18502/ijm.v15i6.14133","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>Bisphenol A (BPA) is a toxic compound with broad applications in the plastics industry. BPA has harmful effects on various organisms and its efficient removal is necessary. The microbial degradation of BPA is a safe and economical approach. In this research, soil samples containing decaying plants were screened to isolate a BPA-degradable bacterial strain.</p><p><strong>Materials and methods: </strong>Soil samples were collected from different locations in Damghan, Semnan province, Iran. To enrich BPA-degrading bacteria, the samples were cultured in a stepwise manner in a mineral medium containing increasing BPA concentrations (5 to 40 mg/L). The ability of isolated bacteria in degrading BPA was assayed by Folin-Ciocalteu and high-performance liquid chromatography methods. The biodegradation efficiency of the most efficient isolate was assayed under distinct conditions and it was identified through the sequencing of the 16S rRNA gene.</p><p><strong>Results: </strong>Among the isolated bacteria, <i>Pseudomonas aeruginosa</i> DU2 (GenBank accession number: OP919484) showed the most BPA biodegradation ability. The highest BPA degradation (52.98%) was observed in the mineral medium containing 5 mg/L BPA and the inoculum size of 6 × 10<sup>7</sup> CFU/mL at pH 9 and in the presence of 0.05% (w/v) NaCl during 10 days.</p><p><strong>Conclusion: </strong>These results offer soil containing decaying plants as a promising source for finding BPA-degrading bacteria. <i>P. aeruginosa</i> DU2 has basal BPA removal ability, which could be improved by optimization of medium components and growth conditions.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10751613/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v15i6.14133","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
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Abstract
Background and objectives: Bisphenol A (BPA) is a toxic compound with broad applications in the plastics industry. BPA has harmful effects on various organisms and its efficient removal is necessary. The microbial degradation of BPA is a safe and economical approach. In this research, soil samples containing decaying plants were screened to isolate a BPA-degradable bacterial strain.
Materials and methods: Soil samples were collected from different locations in Damghan, Semnan province, Iran. To enrich BPA-degrading bacteria, the samples were cultured in a stepwise manner in a mineral medium containing increasing BPA concentrations (5 to 40 mg/L). The ability of isolated bacteria in degrading BPA was assayed by Folin-Ciocalteu and high-performance liquid chromatography methods. The biodegradation efficiency of the most efficient isolate was assayed under distinct conditions and it was identified through the sequencing of the 16S rRNA gene.
Results: Among the isolated bacteria, Pseudomonas aeruginosa DU2 (GenBank accession number: OP919484) showed the most BPA biodegradation ability. The highest BPA degradation (52.98%) was observed in the mineral medium containing 5 mg/L BPA and the inoculum size of 6 × 107 CFU/mL at pH 9 and in the presence of 0.05% (w/v) NaCl during 10 days.
Conclusion: These results offer soil containing decaying plants as a promising source for finding BPA-degrading bacteria. P. aeruginosa DU2 has basal BPA removal ability, which could be improved by optimization of medium components and growth conditions.
背景和目的：双酚 A（BPA）是一种有毒化合物，广泛应用于塑料工业。双酚 A 会对各种生物产生有害影响，因此必须有效地去除双酚 A。微生物降解双酚 A 是一种安全、经济的方法。本研究对含有腐烂植物的土壤样本进行了筛选，以分离出一种可降解双酚 A 的细菌菌株：土壤样本采集自伊朗塞姆南省 Damghan 的不同地点。为了富集双酚 A 酸降解细菌，在含有浓度不断增加的双酚 A 酸（5 至 40 毫克/升）的矿物培养基中逐步培养样品。采用 Folin-Ciocalteu 和高效液相色谱法检测了分离细菌降解双酚 A 的能力。在不同条件下测定了最有效分离菌的生物降解效率，并通过 16S rRNA 基因测序对其进行了鉴定：结果：在分离出的细菌中，铜绿假单胞菌 DU2（GenBank 编号：OP919484）对双酚 A 的生物降解能力最强。在含有 5 mg/L 双酚 A 的矿物培养基中，接种量为 6 × 107 CFU/mL，pH 值为 9，存在 0.05% (w/v) NaCl 的条件下，10 天内观察到的双酚 A 降解率最高（52.98%）：这些结果表明，含有腐烂植物的土壤是寻找双酚 A 降解菌的一个很有前景的来源。铜绿微囊藻 DU2 具有基本的双酚 A 去除能力，可通过优化培养基成分和生长条件来提高这种能力。
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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