Diagnostic evaluation of Tru-Nat MTB/Rif test in comparison with microscopy for diagnosis of pulmonary tuberculosis at tertiary care hospital of eastern Uttar Pradesh.

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2024-08-01 DOI:10.18502/ijm.v16i4.16305
Piyush Ranjan, Atul R Rukadikar, Vivek Hada, Aroop Mohanty, Parul Singh
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Abstract

Background and objectives: This study evaluated the efficacy of the TrueLab™ Real Time mini-PCR system in providing rapid and accurate diagnostic results for tuberculosis (TB) detection in India. The goal is to improve case detection and accelerate treatment in settings with limited resources.

Materials and methods: This prospective study was conducted by the Department of Microbiology on 120 patients, age ranging from >=15 years with at least two clinical symptoms of pulmonary TB. Molbio and Universal Cartridge Based Sample Prep were the 2 methods used for processing sputum samples. The diagnosis was based on the MTB Real Time PCR test, which has a detection limit of 100 CFU/mL. Patients under 15 years, samples lacking clinical background, saliva specimens or extra-pulmonary TB cases were excluded from the study.

Results: A total of 44.17% samples were positive for TB with maximum positivity in the age group 31-45 years. Positivity rate was found to be higher in females. In 4.17% of cases there was rifampicin resistance, which was significantly high in previously treated cases. Comparison of Truenat with Ziehl-Neelsen and fluorescent method revealed that it was more sensitive and less time consuming.

Conclusion: Truenat MTB/RIF is a sensitive detection system for TB with rapid results, which serves as an important tool in the early management of tuberculosis patients and drug-resistant-TB cases.

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在北方邦东部的三级医院中,Tru-Nat MTB/Rif 检测与显微镜检查在肺结核诊断中的比较诊断评估。
背景和目的:本研究评估了 TrueLab™ 实时迷你 PCR 系统为印度结核病 (TB) 检测提供快速准确诊断结果的功效。目的是在资源有限的情况下改进病例检测并加快治疗:这项前瞻性研究是由微生物学系对 120 名年龄大于等于 15 岁、至少有两种肺结核临床症状的患者进行的。Molbio 和通用盒式样本制备是处理痰样本的两种方法。诊断基于 MTB 实时 PCR 检测,其检测限为 100 CFU/mL。研究排除了 15 岁以下患者、缺乏临床背景的样本、唾液样本或肺外结核病例:结果:共有 44.17% 的样本对结核病呈阳性反应,其中 31-45 岁年龄组的阳性率最高。女性的阳性率较高。4.17%的病例对利福平产生耐药性,这在之前接受过治疗的病例中明显较高。将 Truenat 与 Ziehl-Neelsen 和荧光法进行比较后发现,Truenat 更灵敏、更省时:结论:Truenat MTB/RIF 是一种灵敏、快速的结核病检测系统,是结核病患者和耐药结核病例早期管理的重要工具。
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来源期刊
CiteScore
2.40
自引率
7.10%
发文量
96
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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