Soil seed bank is affected by transferred soil thickness and properties in the reclaimed coal mine in the Qilian Mountains, China

Abstract

Reclamation of lands abandoned after mining in mountain areas is critical to erosion control, safety from landslides, and ecological protection of mountain ecosystems. However, little is known about alpine coal mine reclamation using the soil seed bank as a potential source for revegetation. We collected samples of persistent soil seed bank for germination experiments from nine reclaimed sites with different soil cover thicknesses and from six control sites in the Qilian Mountains of China. Soil properties of each site were determined (including soil water content, soil available potassium, soil available phosphorus, soil total nitrogen, pH, soil organic matter, soil total phosphorus, and soil total potassium, and soil alkali-hydrolyzable nitrogen), and the relationships of the characteristics of the soil seed bank with soil cover thickness and soil properties were examined. The results showed that the density, number of species, and diversity of the topsoil seed bank were significantly correlated with soil cover thickness, and all increased with the increment of soil cover thickness. Soil cover thickness controlled the soil seed bank by influencing soil properties. With the increase in soil cover thickness, soil properties (e.g., soil organic matter, soil total nitrogen, etc.) content increased while soil pH decreased. The soil seed bank had the potential to restored the pre-mining habitat at reclaimed sites with approximately 20-cm soil cover thickness. Soil properties of reclaimed sites were lower than that of natural sites. The relationship between the soil seed bank and soil cover thickness determined in this study provides a foundation for improving reclamation measures used in coal mines, as well as for the management and monitoring of reclaimed areas.