Antibacterial effect of Ag-containing hydroxyapatite thin film fabricated by sputtering

IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Bio-medical materials and engineering Pub Date : 2024-01-04 DOI:10.3233/bme-230131
K. Ozeki, Y. Nakajima
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Abstract

BACKGROUND:Infections related to joint prosthesis are still a major concern for orthopedic surgeons. Hydroxyapatite (HA) is a useful biocompatible material because of its good osteocompatibility. Antibacterial HA coatings have been fabricated with addition of antibacterial agents such as Ag to HA using the plasma spraying method. However, the plasma-sprayed HA coating suffers from fractures at large thicknesses. The sputter-coated HA thin film has a high density, and has been applied clinically for dental implants. However, there are no extensive studies on Ag-containing HA thin films. OBJECTIVE:The aim of this study was to prepare an Ag-containing HA thin film by sputtering, and evaluate its antibacterial effect and cytotoxicity. METHODS:The Ag-containing HA thin films were prepared by radio-frequency magnetron sputtering. The films were characterized using X-ray photoelectron spectroscopy, X-ray diffractometry, and scanning electron microscopy (SEM). The antibacterial activities and cytotoxicities of the films were also evaluated. RESULTS:The Ag/Ca molar ratio of the films increased with the Ag ratio in the target. The SEM observation of the hydrothermally treated films showed surfaces covered with globular particles. All Ag-containing HA films exhibited an antibacterial effect against E. Coli. The number of cells of the films decreased with the Ag ratio. The films subjected to the hydrothermal treatment exhibited a higher number of cells than the as-sputtered films. CONCLUSION:The Ag-containing HA thin film was effective in terms of antibacterial effect, and had a low cytotoxicity in the proliferation of osteoblast cells at a low Ag ratio in the target.
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用溅射法制造的含银羟基磷灰石薄膜的抗菌效果
背景:与关节假体有关的感染仍是整形外科医生关注的主要问题。羟基磷灰石(HA)具有良好的骨相容性,是一种有用的生物相容性材料。抗菌 HA 涂层是利用等离子喷涂法在 HA 中添加 Ag 等抗菌剂制成的。然而,等离子喷涂的 HA 涂层在厚度较大时会出现断裂。溅射涂层的 HA 薄膜具有高密度,已被临床应用于牙科植入物。然而,目前还没有关于含银 HA 薄膜的广泛研究。目的:本研究旨在通过溅射法制备含Ag的HA薄膜,并评估其抗菌效果和细胞毒性。方法:采用射频磁控溅射法制备含Ag的HA薄膜。利用 X 射线光电子能谱、X 射线衍射仪和扫描电子显微镜(SEM)对薄膜进行了表征。此外,还对薄膜的抗菌活性和细胞毒性进行了评估。结果:薄膜的Ag/Ca摩尔比随着靶材中Ag比例的增加而增加。水热处理薄膜的扫描电镜观察结果表明,薄膜表面覆盖着球状颗粒。所有含银的 HA 薄膜都对大肠杆菌有抗菌作用。薄膜上的细胞数量随含银量的增加而减少。经过水热处理的薄膜的细胞数要高于溅射薄膜。结论:含Ag的HA薄膜具有有效的抗菌效果,并且在低Ag比例的靶材中对成骨细胞的增殖具有较低的细胞毒性。
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来源期刊
Bio-medical materials and engineering
Bio-medical materials and engineering 工程技术-材料科学:生物材料
CiteScore
1.80
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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