EFFECTS OF INTRAMUSCULAR INJECTION OF PLATELET-RICH PLASMA ON LARVAL BURDEN OF TRICHINELLA SPIRALIS IN EXPERIMENTALLY INFECTED MICE

Abstract

Trichinellosis (= trichinosis) is widespread food-borne zoonotic parasitosis caused by the nematode Trichinella spiralis triggering skeletal muscle injury. This study identified the therapeutic effects of platelet-rich plasma (PRP) as a safe choice in muscular phase of mice experimental infected with T. spiralis . Ninety male mice were divided into three main groups, GI: Negative con-trol, GII: Positive control and GIII: Trichinella infected PRP treated. All mice were sacrificed on 14 th day post infection (initiation of muscle cell invasion), on 21 st day (early stage of nurse cell formation), on 28 th day (formation of most nurse cells was completed), and on 35 th day PI (convalescent period). The parasitological examination showed a significant reduction in the number of larvae detected in the infected PRP-treated ones 35 th PI. Histopathological and immunohistochemical of vascular endothelial growth factor, caspase 3 analyses coincided with parasitological findings, on 35 th day PI as well. There was a significant improvement in the in-flammatory cells with fewer muscle fibers degradation, indicating that PRP injection in trichinellosis muscular phase improved inflammatory reaction and reduce T. spiralis larval burden.