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ULTRASTRUCTURE OF VITELLOGENESIS AND VITELLOCYTES IN LECITHOCHIRIUM GRANDID ON SAURIDA UNDOSQUAMIS, TRAPPED FROM ALATAKA HARBOR, SUEZ GULF, EGYPT 从埃及苏伊士湾阿拉塔卡港捕获的大鳞蜥卵黄发生和卵黄细胞的超微结构
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331726
Shereen A. Fahmy, Salwa Z. A. Arafa
The study gave TEM ultrastructural of vitellogenesis in Lecithochirium grandid , digenean intestinal parasite of Saurida undosquamis , four developmental phases (1, 2, 3 & 4) during vitel-logenesis. Vitelline bundles were enveloped with fine fibrous layer surrounded with separ-ated circular muscle bands. Ultrastructural study showed that vitellocyte maturation comprised of increased extension of parallel cisternae of GER, increase in cell size and in the different phases increased numbers of pellets aggregation in clusters during vitellogenesis. In mature vitel-locytes, there were large numbers of various aggregations such as aggregation of pellets in clusters; the important portion in construction of egg shell and a number of unsaturated lipid droplets. This agreed with different phases of oocyte development in parasitic Platyhelminthes.
该研究通过 TEM 超微结构观察了金龙鱼肠道寄生虫 Lecithochirium grandid 的卵黄发生过程,卵黄发生过程分为四个发育阶段(1、2、3 和 4)。玻璃体束被细纤维层包裹,周围有分离的环形肌带。超微结构研究表明,玻璃体细胞的成熟包括玻璃体细胞发生过程中GER平行腔的扩展、细胞体积的增大以及在不同阶段聚集成团的颗粒数量的增加。在成熟的卵黄细胞中,存在大量的各种聚集体,如聚集成团的颗粒;这是构建蛋壳的重要部分,也是大量的不饱和脂滴。这与寄生性扁形动物卵母细胞发育的不同阶段一致。
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引用次数: 0
CHIKUNGUNYA VIRUS VERSUS AEDES-BORNE OTHER VIRUSES: A REVIEW ARTICLE 基孔肯雅病毒与伊蚊传播的其他病毒:综述文章
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331733
Tosson Morsy, Mohamed Elgohary, T. Sallam, Ayman Morsy
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引用次数: 0
Index 索引
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331915
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引用次数: 0
ENDOSCOPIC ULTRASOUND ELASTOGRAPHY IN ASSESSMENT OF LYMPH NODES IN PATIENTS WITH HEPATOCELLULAR CARCINOMA PREPARED FOR LIVING DONOR LIVER TRANSPLANTATION 内窥镜超声弹性成像技术在评估准备接受活体肝移植的肝细胞癌患者淋巴结方面的应用
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331729
Abdelaziz Mahmoud, Mohga Reda, Amany Ibrahim, Manar SALAH-ELDIN
Lymph nodes are the most frequent site of extrahepatic metastases for hepatocellular carci- - cal ones from those best suited for non-surgical management. Diagnosis cannot be completely confirmed by imaging and operative exploration. Endoscopic ultrasound elastography (EUS-EG) has a marked role in assessment of lymph nodes in HCC patients and optimizing their management accordingly. This study assessed diagnostic accuracy of EUS-EG in suspected lymph nodes in HCC potential candidates for living donor liver transplantation (LDLT) and identifued its role in diagnosis and staging of hepatocellular carcinoma. The study was conducted at the Tropical Medicine department, Ain Shams University, Ain Shams Center of Organ Transplantation (ASCOT) and Endoscopy Unit, Theodor Bilharz Research Institute between July 2018 & May2023. The patients 24 with HCC and abdominal lymphadenopathy were prepared for LDLT. Liver function tests, Positron emission tomography (PET) scan, EUS-EG, EUS-guided fine needle aspiration (EUS-FNA). The results showed the prevalence of metastatic lymph nodes by EUS-FNA was 41.7%. EUS-EG and PET scan had same sensitivity of 80.0% in detecting metastatic lymph nodes. But, EUS-EG had a higher specificity 85.7% compared to 57.1% specificity of PET scan.
淋巴结是肝细胞钙化最常见的肝外转移部位,也是最适合非手术治疗的部位。影像学检查和手术探查无法完全确诊。内镜超声弹性成像(EUS-EG)在评估肝癌患者淋巴结并相应优化治疗方面具有显著作用。这项研究评估了 EUS-EG 对可能接受活体肝移植(LDLT)的 HCC 候选者中疑似淋巴结的诊断准确性,并确定了它在肝细胞癌诊断和分期中的作用。该研究于2018年7月至2023年5月期间在艾因夏姆斯大学热带医学系、艾因夏姆斯器官移植中心(ASCOT)和Theodor Bilharz研究所内窥镜室进行。24 名患有 HCC 和腹腔淋巴结病的患者为 LDLT 做好了准备。肝功能检查、正电子发射断层扫描(PET)、EUS-EG、EUS引导下细针穿刺(EUS-FNA)。结果显示,通过 EUS-FNA 检查发现转移淋巴结的比例为 41.7%。EUS-EG 和 PET 扫描检测转移淋巴结的敏感性相同,均为 80.0%。但 EUS-EG 的特异性为 85.7%,而 PET 扫描的特异性为 57.1%。
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引用次数: 0
COMPARISON BETWEEN GROWTH MAGNITUDE OF BLASTOCYSTIS GENOTYPES ISOLATED FROM GASTROINTESTINAL SYMPTOMATIC EGYPTIAN PATIENTS 从埃及胃肠道症状患者体内分离出的囊胚孢子菌基因型的生长速度比较
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331728
Maha Alabbassy, Eman Hussein, Sherine Elzaghawy
Blastocystis presents in healthy and diseased persons so its role in disease development is a subject of debate. This study compared between growth patterns of different symptomatic Blastocystis subtypes. Morphometric measures of the vacuolar form of STs isolated from acute GIT symptomatic patients showed that major diversity in size ranged from 4.928±1.77 among ST4 to 70.54±29.66 among wild ST3. Size in chronic group was 4±0.816 in ST-4 and 40.83±7.359 in heterozygous ST3, and in intermittent group ranged from 5.5±0.707 in ST4 to 47.5±6.45 in heterozygous ST3 with significant differences. Phenotypic variations were commonly among inter-subtypes of ST3. Generation patterns in re-culturing were 24.1% of isolates at 5 th re-culturing and the 29.6% at 6 th re-culturing loss of 90% viable vacuolar form numbers compared to that on first culture. Low percenta ge (9.3%) reached to 9 th re-culture. Majority of ST-1 (40%) was lost at the 5 th re-culture while 58.8% of wild ST-3 was lost in the 7 th & 8 th , respectively, with 29.4% per each. Meanwhile, 66.6% of ST-4 was lost in the 5 th & 6 th , respectively, with 33.3% per each, but without significant difference. As to metronidazole ® susceptibility, the majority of ST1, wild ST3, heterozygous ST3 and ST4 isolates gave 60%, 58.7%, 57.2% & 79.9% sensitivity.
健康人和病人体内都有母包虫,因此母包虫在疾病发展中的作用还存在争议。本研究比较了不同症状子囊菌亚型的生长模式。从急性胃肠道症状患者体内分离出的空泡型ST的形态测量结果显示,ST4的大小差异很大,从4.928±1.77到野生ST3的70.54±29.66不等。在慢性组中,ST-4 的大小为 4±0.816,杂合 ST3 为 40.83±7.359;在间歇组中,ST-4 为 5.5±0.707,杂合 ST3 为 47.5±6.45,差异显著。ST3 亚型间的表型差异很常见。再培养的生成模式是,与第一次培养相比,24.1%的分离物在第 5 次再培养时丧失了 90% 的有活力空泡形态数,而在第 6 次再培养时丧失了 29.6% 的有活力空泡形态数。在第 9 次再培养时,有较低的百分比(9.3%)达到了再培养水平。大多数 ST-1(40%)在第 5 次再培养时损失,而 58.8%的野生 ST-3 在第 7 次和第 8 次再培养时分别损失了 29.4%。与此同时,ST-4 在第 5 次和第 6 次再培养中分别损失了 66.6%和 33.3%,但没有显著差异。至于对甲硝唑 ® 的敏感性,大多数 ST1、野生 ST3、杂合 ST3 和 ST4 分离物的敏感性分别为 60%、58.7%、57.2% 和 79.9%。
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引用次数: 0
DETECTION AND IDENTIFICATION OF TOXOCARA CANIS IN DOGS IN TABUK REGION, SAUDI ARABIA BY PCR AND DNA SEQUENCING 通过 PCR 和 DNA 测序检测和鉴定沙特阿拉伯塔布克地区狗体内的犬弓形虫
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331731
Areej AL-GHABBAN
Dog feces are important for spreading and transmitting parasite eggs, larvae, and oocysts by contaminating soil, food, or water. Dog feces can be a source of intestinal parasites. This study investigated the prevalence of Toxocara canis infection in stray dogs in the Tabuk Region in northwest Saudi Arabia. Eggs were discovered using the sedimentation flotation method in the feces of stray dogs, and they were recognized using DNA sequencing and polymerase chain reaction (PCR). Out of the 200 fecal samples that were randomly obtained from public places, the Tabuk region's west (an agricultural area) showed the highest prevalence of eggs (7.5%). The molecular approaches for identification of this parasite are sensitive sufficient to distinguish be-tween the various T. canis in feces and to detect low parasite levels. Due to Saudi Arabia's ineffective environmental hygiene controls, the comparatively high prevalence of this parasite may continue to rise. This study accurately identified T. canis in dogs in Tabuk Region by a new technique, eliminating the possibility of misdiagnosis by traditional fecal microscopy.
狗的粪便会污染土壤、食物或水,从而传播寄生虫卵、幼虫和卵囊。狗粪可能是肠道寄生虫的来源之一。本研究调查了沙特阿拉伯西北部塔布克地区流浪狗的犬弓形虫感染率。采用沉淀浮选法在流浪狗的粪便中发现了虫卵,并通过 DNA 测序和聚合酶链反应 (PCR) 对其进行了识别。在从公共场所随机获取的 200 份粪便样本中,塔布克地区西部(农业区)的虫卵感染率最高(7.5%)。鉴定这种寄生虫的分子方法灵敏度高,足以区分粪便中的各种犬蛔虫,并能检测到低水平的寄生虫。由于沙特阿拉伯的环境卫生控制不力,这种寄生虫的相对高流行率可能会继续上升。这项研究采用一种新技术准确鉴定了塔布克地区狗体内的犬结核菌,消除了传统粪便显微镜检查误诊的可能性。
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引用次数: 0
INVESTIGATION OF IMMUNOLOGICAL RESPONSE OF EGYPTIAN BEE VENOM PREPARATION IN RABBITS 埃及蜂毒制剂对兔子免疫反应的研究
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331735
Abir Elfiky, Eman Abououf
The repeated exposure of the immune system to bee venom as antigen leads to significant increase in immune response. In this study different immunological schedules were tried to reach maximum immunological effect with the least allergic side effect either by using and aqueous or emulsified preparation of bee venom. Three groups of male rabbits of one year old each were used for a period of four weeks. The blood samples were with drown before bee venom injection and weekly after its infection for determination of IgG and IgE. The results showed that injection of Egyptian bee venom in an aqueous form have the ability to boost the level of immune response without significant allergic reaction.
免疫系统反复接触作为抗原的蜂毒,会导致免疫反应显著增强。本研究尝试了不同的免疫学方案,通过使用蜂毒的水溶液或乳化制剂来达到最大的免疫效果和最小的过敏副作用。研究使用了三组雄性兔子,每组一岁,为期四周。在注射蜂毒前和感染蜂毒后每周对兔子进行一次血液采样,以测定 IgG 和 IgE。结果表明,注射埃及蜂毒水剂能够提高免疫反应水平,而不会产生明显的过敏反应。
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引用次数: 0
EFFECT OF AN EDUCATIONAL PROGRAM ON KNOWLEDGE AND PRACTICE OF NURSES CARING FOR NEPHROTIC CHILDREN 教育计划对肾病患儿护理护士的知识和实践的影响
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331743
Suzan EL-SHAHAT, Nagwa Aly, Ahmed Ahmad
Pediatric nurses had unsatisfactory knowledge and practice as to care of nephritis syndrome children. The study evaluated the effect of an-educational program on knowledge and practice of nurses caring for nephritis children. A quasi-experimental design was utilized on 57 pediatric nurses caring for nephritis syndrome children. Two tools were utilized for data collection; self-administered questionnaire sheet included personal characteristics of pediatric nurses, knowledge, and observational checklist for evaluation the nursing practices of children. The results showed that majority of them had unsatisfactory knowledge and inadequate practice about nephritis syndrome before program implementation. This was significantly improved in immediate and 3 months after program implementation, with a positive correlation between nurses' knowledge, age and years of experience, also between nurses' practice, age, pediatric year and general experience.
儿科护士在护理肾炎综合征患儿方面的知识和实践都不尽如人意。这项研究评估了教育计划对护理肾炎患儿的护士的知识和实践的影响。研究采用准实验设计,对象是 57 名护理肾炎综合征患儿的儿科护士。数据收集使用了两种工具:包括儿科护士个人特征、知识在内的自填式问卷和用于评估儿童护理实践的观察清单。结果显示,在计划实施前,大多数儿科护士对肾炎综合征的认识和实践不足。护士的知识水平、年龄和工作年限之间以及护士的工作方法、年龄、儿科年限和一般工作经验之间呈正相关。
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引用次数: 0
THERAPEUTIC EFFICACY OF RESIQUIMOD ON TREATING CHRONIC TOXOPLASMOSIS IN EXPERIMENTAL INFECTED MICE 雷喹莫德治疗实验感染小鼠慢性弓形虫病的疗效
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331739
R. Brakat, N. Badr, S. Soliman, S. Sharaf-El-Deen
Toxoplasmosis is a zoonotic protozoan infectious disease affects people worldwide with a high rate of morbidity and mortality. The incomplete efficacy of the approved drugs for the chronic Toxoplasma gondii infections endangers one-third of the global humans and animals with reactivation. This is due to the risky complications chronic toxoplasmosis causes. This study evaluated the TLRs 7/8 agonist, Resiquimod ® for treating chronic toxoplasmosis in BALB/c mice infected with the ME49 strain of T. gondii as compared the traditionally used Py-rimethamine ® & Sulphadiazine ® . This was judged by the number of brain cysts, the histpatholo-gical degrees in the brain and retina, expression of the apoptotic marker, caspase-3 compared to negative and positive controls. The results showed a statistically significant decrease in the number of brain cysts in resiq-uimod-treated mice correlated with the histopathological improvement of brain and retina and apoptosis.
弓形虫病是一种人畜共患的原生动物传染病,影响着世界各地的人们,发病率和死亡率都很高。由于已批准的治疗慢性弓形虫感染的药物疗效不佳,全球三分之一的人类和动物都面临着弓形虫再活化的危险。这是由于慢性弓形虫病会导致危险的并发症。本研究评估了 TLRs 7/8 激动剂 Resiquimod ® 治疗感染 ME49 株弓形虫的 BALB/c 小鼠慢性弓形虫病的效果,并与传统使用的 Py-rimethamine ® 和 Sulphadiazine ® 进行了比较。与阴性和阳性对照组相比,该药物可通过脑囊肿的数量、大脑和视网膜的组织病理学程度、细胞凋亡标志物 caspase-3 的表达来进行判断。结果表明,经 resiq-uimod 处理的小鼠脑囊肿数量在统计学上显著减少,这与大脑和视网膜的组织病理学改善及细胞凋亡有关。
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引用次数: 0
SPOTLIGHT ON LEISHMANIASIS, PHLEBOTOMUS VECTORS AND ANIMAL RESERVOIRS IN EGYPT 聚焦埃及的利什曼病、血吸虫病媒和动物水库
Pub Date : 2023-12-01 DOI: 10.21608/jesp.2023.331744
Tosson Morsy, S. Dahesh
Small-sized sand-flies about 3 mm in length are typical for many species, which helps them in the escaping notice. Females suck blood from various mammals including man, reptiles and birds, which bite is not always felt, but leaves a small round, reddish bump that starts itching hours or days later. They transmit bacterial parasitic and viral infectious diseases
许多种类的沙蝇体型较小,长约 3 毫米,这有助于它们逃跑。雌性沙蝇从各种哺乳动物(包括人类、爬行动物和鸟类)身上吸血,被叮咬后不一定能感觉到,但会留下一个淡红色的小圆疙瘩,数小时或数天后开始发痒。它们传播细菌性寄生虫病和病毒性传染病
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引用次数: 0
期刊
Journal of the Egyptian Society of Parasitology
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