Determination of in vitro thrombolytic activity in isolated flavonoids of plant Madras Nelli

Abstract

The study is regarded for standardization of aerial parts of the plant Phyllanthus maderaspatensis L., phytochemical investigations, isolation of flavonoids and evaluation for thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract. Standardization study includes macroscopy, microscopy and determination of proximate values and phytochemical investigations include extraction of coarsely powdered herb with exhaustive alcoholic and aqueous extractions and successive extraction with petroleum ether, chloroform and alcohol. The extracts were subjected to qualitative chemical analysis, chromatographic studies [Thin Layer Chromatography (TLC) and High Performance Thin Layer Chromatography (HPTLC)] were performed for flavonoids detection in alcoholic extract. Isolated two flavonoids from butanolic fraction of alcoholic extract by column chromatography and preparative TLC, their partial characterization was done with Proton Nuclear Magnetic Resonance (PNMR) and Fourrier Transform Infrared (FT-IR) spectral data. Thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract (20 and 10 mg/100 µl) were evaluated in incubated blood. An attempt made to standardize aerial parts of P. maderaspatensis L., was successful. Two flavonoids were isolated; the alcoholic extract showed significant thrombolytic effect in comparison with thrombosis control in comparison with standard thrombolytic agent streptokinase (30000 unit/100 µl).