Mamfe Sakyiamah Maxwell, Kyei Baffour Prince, Atta-Adjei Peter, Bolah Peter, Ehun Ebenezer, Opare Christiana, Brew-Daniels Henry, Ampomah Appiah Alfred
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{"title":"Effect of seasonal variations on the secondary metabolites and antioxidant activities of Bridelia ferruginea, Lippia multiflora, and Azadirachta indica leaves","authors":"Mamfe Sakyiamah Maxwell, Kyei Baffour Prince, Atta-Adjei Peter, Bolah Peter, Ehun Ebenezer, Opare Christiana, Brew-Daniels Henry, Ampomah Appiah Alfred","doi":"10.5897/jpp2023.0624","DOIUrl":"https://doi.org/10.5897/jpp2023.0624","url":null,"abstract":".","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study is regarded for standardization of aerial parts of the plant Phyllanthus maderaspatensis L., phytochemical investigations, isolation of flavonoids and evaluation for thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract. Standardization study includes macroscopy, microscopy and determination of proximate values and phytochemical investigations include extraction of coarsely powdered herb with exhaustive alcoholic and aqueous extractions and successive extraction with petroleum ether, chloroform and alcohol. The extracts were subjected to qualitative chemical analysis, chromatographic studies [Thin Layer Chromatography (TLC) and High Performance Thin Layer Chromatography (HPTLC)] were performed for flavonoids detection in alcoholic extract. Isolated two flavonoids from butanolic fraction of alcoholic extract by column chromatography and preparative TLC, their partial characterization was done with Proton Nuclear Magnetic Resonance (PNMR) and Fourrier Transform Infrared (FT-IR) spectral data. Thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract (20 and 10 mg/100 µl) were evaluated in incubated blood. An attempt made to standardize aerial parts of P. maderaspatensis L., was successful. Two flavonoids were isolated; the alcoholic extract showed significant thrombolytic effect in comparison with thrombosis control in comparison with standard thrombolytic agent streptokinase (30000 unit/100 µl).
该研究旨在对植物 Phyllanthus maderaspatensis L. 的气生部分进行标准化、植物化学研究、黄酮类化合物的分离以及酒精提取物和酒精提取物丁醇部分的溶栓活性评估。标准化研究包括宏观镜检、显微镜检和近似值测定，而植物化学研究则包括对粗粉末药草进行提取，包括彻底的酒精提取和水提取，以及石油醚、氯仿和酒精的连续提取。对提取物进行了定性化学分析，并对酒精提取物中的黄酮类化合物进行了色谱分析（薄层色谱法（TLC）和高效薄层色谱法（HPTLC））。通过柱层析和制备型 TLC 从酒精提取物的丁醇馏分中分离出两种黄酮类化合物，并利用质子核磁共振（PNMR）和傅立叶变换红外光谱（FT-IR）数据对其进行了部分表征。在培养血液中评估了酒精提取物和酒精提取物丁醇部分（20 和 10 mg/100 µl）的溶栓活性。对 P. maderaspatensis L. 的气生部分进行标准化的尝试取得了成功。分离出了两种黄酮类化合物；酒精提取物与标准溶血剂链激酶（30000 单位/100 µl）相比，具有明显的溶栓效果。
{"title":"Determination of in vitro thrombolytic activity in isolated flavonoids of plant Madras Nelli","authors":"Raj Padhya Ramesh, Maharjan Sajan, Khanal Amrit, Pokhrel Thakur, Krishna Burdipad Gopala","doi":"10.5897/jpp2019.0565","DOIUrl":"https://doi.org/10.5897/jpp2019.0565","url":null,"abstract":"The study is regarded for standardization of aerial parts of the plant Phyllanthus maderaspatensis L., phytochemical investigations, isolation of flavonoids and evaluation for thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract. Standardization study includes macroscopy, microscopy and determination of proximate values and phytochemical investigations include extraction of coarsely powdered herb with exhaustive alcoholic and aqueous extractions and successive extraction with petroleum ether, chloroform and alcohol. The extracts were subjected to qualitative chemical analysis, chromatographic studies [Thin Layer Chromatography (TLC) and High Performance Thin Layer Chromatography (HPTLC)] were performed for flavonoids detection in alcoholic extract. Isolated two flavonoids from butanolic fraction of alcoholic extract by column chromatography and preparative TLC, their partial characterization was done with Proton Nuclear Magnetic Resonance (PNMR) and Fourrier Transform Infrared (FT-IR) spectral data. Thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract (20 and 10 mg/100 µl) were evaluated in incubated blood. An attempt made to standardize aerial parts of P. maderaspatensis L., was successful. Two flavonoids were isolated; the alcoholic extract showed significant thrombolytic effect in comparison with thrombosis control in comparison with standard thrombolytic agent streptokinase (30000 unit/100 µl).","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139203421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mawira Kelvin, J.N Ngeranwa Joseph, Mwonjoria John, Nginyi Joseph
Helminthiasis causes losses in livestock production, and anthelminthics are used to treat helmintic infections. Resistance has necessitated the need for development of more effective alternatives. Plants offer a promising alternative and, in Meru County, traditional plant-based treatments for helminthiasis have been used, although there is a lack of scientific proof of their efficacy. The aim of this study was to investigate efficacy of Bridelia micrantha, Aframomum zambesiacum, Hagenia abyssinica, Rubus apetalus, Thespecia garckeana, Physalis peruviana and Caesalpina volkensii against Haemonchus contortus. It entailed screening of methanolic extracts of these plants against Haemonchus contortus, from sheep farms in Muguga Kenya. Their efficacy was tested in-vitro using eggs and larvae of Haemonchus contortus. Egg hatchability was determined after 48-h incubation with extracts while larvae survival was determined after six days incubation. Physiological saline was the negative control while albendazole was the positive control. One-way ANOVA was conducted followed by Tukey’s test. P. peruviana and R. apetalus, inhibition percentages of 95.24±0.54, 90.00±1.00, 88.24±0.66 and 96.55±0.45, 85.71±0.79, 82.14±0.76 at 50, 25 and 12.5 mg/ml respectively was achieved with no significant difference (P<0.05) in egg hatchability with the positive control. The highest larvicidal mean percentage of 100.00±0.00 was achieved by R. apetalus and H. abyssinica across the three dosages with no significant difference between them and albendazole. GC-MS analysis revealed compounds such as terpenoids were conspicuously present which accounted for some of the activity. This observation lenders support to the traditional use of the plant extracts for the treatment of suspected helminthic infections. Key words: Haemonchus contortus, anthelmintic, essential oil, monoterpenoids, terpenes.
{"title":"In vitro anthelmintic activity against Haemonchus contortus of methanolic extracts of selected medicinal plants from Meru County, Kenya","authors":"Mawira Kelvin, J.N Ngeranwa Joseph, Mwonjoria John, Nginyi Joseph","doi":"10.5897/jpp2023.0627","DOIUrl":"https://doi.org/10.5897/jpp2023.0627","url":null,"abstract":"Helminthiasis causes losses in livestock production, and anthelminthics are used to treat helmintic infections. Resistance has necessitated the need for development of more effective alternatives. Plants offer a promising alternative and, in Meru County, traditional plant-based treatments for helminthiasis have been used, although there is a lack of scientific proof of their efficacy. The aim of this study was to investigate efficacy of Bridelia micrantha, Aframomum zambesiacum, Hagenia abyssinica, Rubus apetalus, Thespecia garckeana, Physalis peruviana and Caesalpina volkensii against Haemonchus contortus. It entailed screening of methanolic extracts of these plants against Haemonchus contortus, from sheep farms in Muguga Kenya. Their efficacy was tested in-vitro using eggs and larvae of Haemonchus contortus. Egg hatchability was determined after 48-h incubation with extracts while larvae survival was determined after six days incubation. Physiological saline was the negative control while albendazole was the positive control. One-way ANOVA was conducted followed by Tukey’s test. P. peruviana and R. apetalus, inhibition percentages of 95.24±0.54, 90.00±1.00, 88.24±0.66 and 96.55±0.45, 85.71±0.79, 82.14±0.76 at 50, 25 and 12.5 mg/ml respectively was achieved with no significant difference (P<0.05) in egg hatchability with the positive control. The highest larvicidal mean percentage of 100.00±0.00 was achieved by R. apetalus and H. abyssinica across the three dosages with no significant difference between them and albendazole. GC-MS analysis revealed compounds such as terpenoids were conspicuously present which accounted for some of the activity. This observation lenders support to the traditional use of the plant extracts for the treatment of suspected helminthic infections. Key words: Haemonchus contortus, anthelmintic, essential oil, monoterpenoids, terpenes.","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135765217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dracaena steudneri Engl. (family Dracaenaceae) has been used in managing various health conditions. This study evaluated its pharmacognostic, physicochemical and phytochemical parameters. The physicochemical analysis was done using WHO recommended parameters such as moisture content, ash values (total ash, water soluble ash, acid insoluble ash) and extractive values. Phytochemical screening was done by methods described by Sofowora, Kokate and Prashant. The morphological studies exhibited the macroscopic characters while the microscopic study showed the presence of various characteristics such as vascular bundles, calcium oxalate crystals and paracytic stomata. Physicochemical evaluation indicated 13.7% total yield, 9.13% moisture content, 0.17% water soluble ash, 0.17% acid insoluble ash, 3.41% water insoluble ash, 0.84% acid insoluble ash, 16.25% acid soluble extractive value and 20% water soluble extractive value. The qualitative phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, saponins, tannins, glycosides and phenols in the extract. The pharmacognostic characters described in this study will help in identifying the plant and crude drug. The standardization parameters obtained will ensure the efficacy of the drug and also distinguish the drug from its adulterants. Key words: Dracaena steudneri, physicochemical, phytochemical, organoleptic evaluation.
{"title":"Physicochemical, phytochemical and pharmacognostical parameters of a herbal plant Dracaena steudneri Engl.","authors":"Gladys Tenywa Mercy, Ganafa Agaba Amon, Umba Tolo Casim, Olusoji Ajayi Clement, Katuura Esther","doi":"10.5897/jpp2021.0611","DOIUrl":"https://doi.org/10.5897/jpp2021.0611","url":null,"abstract":"Dracaena steudneri Engl. (family Dracaenaceae) has been used in managing various health conditions. This study evaluated its pharmacognostic, physicochemical and phytochemical parameters. The physicochemical analysis was done using WHO recommended parameters such as moisture content, ash values (total ash, water soluble ash, acid insoluble ash) and extractive values. Phytochemical screening was done by methods described by Sofowora, Kokate and Prashant. The morphological studies exhibited the macroscopic characters while the microscopic study showed the presence of various characteristics such as vascular bundles, calcium oxalate crystals and paracytic stomata. Physicochemical evaluation indicated 13.7% total yield, 9.13% moisture content, 0.17% water soluble ash, 0.17% acid insoluble ash, 3.41% water insoluble ash, 0.84% acid insoluble ash, 16.25% acid soluble extractive value and 20% water soluble extractive value. The qualitative phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, saponins, tannins, glycosides and phenols in the extract. The pharmacognostic characters described in this study will help in identifying the plant and crude drug. The standardization parameters obtained will ensure the efficacy of the drug and also distinguish the drug from its adulterants. Key words: Dracaena steudneri, physicochemical, phytochemical, organoleptic evaluation.","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Kiprono, G. Mengich, B. Ondigo, Charles Mutai, S. Kimoloi
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{"title":"Therapeutic uses of stingless bee honey by traditional medicine practitioners in Baringo County, Kenya","authors":"S. Kiprono, G. Mengich, B. Ondigo, Charles Mutai, S. Kimoloi","doi":"10.5897/jpp2022.0618","DOIUrl":"https://doi.org/10.5897/jpp2022.0618","url":null,"abstract":".","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85875521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cherif-Soumahoro Aicha, Chantal N’GAMAN-KOUASSI Christelle, Akhanovna MAMYRBEKOVA-BEKRO Janat, Pirat Jean-Luc, V. David, B. Yves-Alain
This work was carried out to evaluate the total phenolic content and antioxidant potential (2,2-di-phenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] methods) of Bauhinia monandra through its organs (leaves, stems and roots). The results showed fluctuating contents with a dominance in the leaves of total phytophenols in the crude hydromethanolic and selective AcOEt extracts (57.344 ± 12.35 and 48.165 ± 4.77 mg EAG/g DM, respectively) and of total flavonoids in the AcOEt extract (100.588 ± 12.06 mg EQ/g DM). The evaluation of antioxidant activity shows that the AcOEt extract of the stems is the most active with a CR50 = 23.442 µg/ml (DPPH method) and 44.34 ± 0.1 µg EVc/100 g DM and 182394.94 ± 406.09 µg EFeSO4/g DM (FRAP method).
{"title":"Evaluation of the phenolic content and in vitro antioxidant potential of Bauhinia monandra Kurz (Fabaceae)","authors":"Cherif-Soumahoro Aicha, Chantal N’GAMAN-KOUASSI Christelle, Akhanovna MAMYRBEKOVA-BEKRO Janat, Pirat Jean-Luc, V. David, B. Yves-Alain","doi":"10.5897/jpp2022.0622","DOIUrl":"https://doi.org/10.5897/jpp2022.0622","url":null,"abstract":"This work was carried out to evaluate the total phenolic content and antioxidant potential (2,2-di-phenyl-1-picrylhydrazyl [DPPH] and ferric reducing antioxidant power [FRAP] methods) of Bauhinia monandra through its organs (leaves, stems and roots). The results showed fluctuating contents with a dominance in the leaves of total phytophenols in the crude hydromethanolic and selective AcOEt extracts (57.344 ± 12.35 and 48.165 ± 4.77 mg EAG/g DM, respectively) and of total flavonoids in the AcOEt extract (100.588 ± 12.06 mg EQ/g DM). The evaluation of antioxidant activity shows that the AcOEt extract of the stems is the most active with a CR50 = 23.442 µg/ml (DPPH method) and 44.34 ± 0.1 µg EVc/100 g DM and 182394.94 ± 406.09 µg EFeSO4/g DM (FRAP method).","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75126772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. N. Mathias, Baba Aminu Abubakar, H. Mshelia, I. Malami
Acacia nilotica (Linn) is an important medicinal plant, found in Africa, the Middle East, and the Indian subcontinent as well as in Sokoto State, Nigeria. It has been utilized to treat wounds and intestinal disorders among other uses. It has been estimated that nearly 6 million people suffer from chronic wounds worldwide. Even though several conventional drugs are known to increase healing in different kinds of injuries, most are complicated, expensive, and limited in activity. Hence, in this study the stem back crude methanolic extract of A. nilotica was evaluated for wound healing activity using the wound excision model. The plant's stem bark was extracted by maceration using distilled water and methanol to obtain a crude methanol extract (CME). The CME was screened phytochemically and prepared into ointment before subjecting to wound healing assay using the excision wound model in Wister albino rats. The results showed the presence of rich phenolic phytoconstituents, especially of tannins and saponins which were attributed to aid wound healing based on literature. Consequently, the wound healing activity of 10% CME was comparable significantly (P<0.05), to that of povidone-iodine (5% w/w), while that of 2. 5 and 5% were less. The study demonstrated that topical application of CME hastens wound healing and thus provided proof of its folklore use for wound healing
{"title":"Bioactive evaluation for wound healing of stem back extracts of Acacia nilotica Linn. (Fabaceae)","authors":"S. N. Mathias, Baba Aminu Abubakar, H. Mshelia, I. Malami","doi":"10.5897/jpp2022.0621","DOIUrl":"https://doi.org/10.5897/jpp2022.0621","url":null,"abstract":"Acacia nilotica (Linn) is an important medicinal plant, found in Africa, the Middle East, and the Indian subcontinent as well as in Sokoto State, Nigeria. It has been utilized to treat wounds and intestinal disorders among other uses. It has been estimated that nearly 6 million people suffer from chronic wounds worldwide. Even though several conventional drugs are known to increase healing in different kinds of injuries, most are complicated, expensive, and limited in activity. Hence, in this study the stem back crude methanolic extract of A. nilotica was evaluated for wound healing activity using the wound excision model. The plant's stem bark was extracted by maceration using distilled water and methanol to obtain a crude methanol extract (CME). The CME was screened phytochemically and prepared into ointment before subjecting to wound healing assay using the excision wound model in Wister albino rats. The results showed the presence of rich phenolic phytoconstituents, especially of tannins and saponins which were attributed to aid wound healing based on literature. Consequently, the wound healing activity of 10% CME was comparable significantly (P<0.05), to that of povidone-iodine (5% w/w), while that of 2. 5 and 5% were less. The study demonstrated that topical application of CME hastens wound healing and thus provided proof of its folklore use for wound healing","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74251339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdou-Rachidou Gaffo, Yendubé T. Kantati, K. K. Koukoura, A. E. Akpalo, Toukilnan Djiwa, T. Darré, K. Metowogo, K. Eklu-Gadégbéku
Adansonia digitata is a tree plant used in west African local pharmacopoeia. This work aimed to evaluate the wound healing properties of its trunk bark’s hydroethanolic extract in wistar rats. The model of excision wound was used. Wistar rats were treated post-excision topically with neutral carbopol gel (Control), carbopol gels containing 2.5 and 5% of A. digitata hydroethanolic trunk bark extract (ADHE) and L-Mesitran. Pictures were taken post excision for analysis. Biopsies were realized for histological examination of the skin excised and for hydroxyproline determination. In vitro membrane stabilization anti-inflammatory test, DPPH and FRAP antioxidant tests, and phytochemical tests were performed. The results show a better rate of contraction in ADHE 2.5 and 5% animals (+56 ± 0.52% and +68 ± 0.24% respectively) as earlier as on day three post-excision. The hydroxyproline assay confirmed a better collagen production in ADHE 2.5% and 5% animals (+102.5 ± 2.9%, and +107 ± 3.0% respectively). Histological analysis showed a rapid re-epithelialization in ADHE animals. The anti-inflammatory test indicated an IC50 of 16.521 ± 0.525 µg/mL, very close to Aspirin (16.826 ± 0.341 µg/mL), showing a possible anti-inflammatory activity of ADHE. The extract reduced DPPH radical (IC50 of 344.985 ± 17.139 µg/mL) and ferric ions (EC50 = 178.689 ± 0.250 µg/mL). Phytochemical investigations revealed the presence of alkaloids, terpenoids, and polyphenolic compounds. Flavonoids in particular, with an amount of 51.694 ± 0.174 mgER/g, should explain the wound healing activity observed, making the hydroethanolic extract of A. digitata a potential remedy for excision wounds.
{"title":"Hydroalcoholic extract of Adansonia digitatas trunk bark, in topical application, possess in vivo wound healing and in vitro anti-inflammatory and antioxidant activities","authors":"Abdou-Rachidou Gaffo, Yendubé T. Kantati, K. K. Koukoura, A. E. Akpalo, Toukilnan Djiwa, T. Darré, K. Metowogo, K. Eklu-Gadégbéku","doi":"10.5897/jpp2022.0620","DOIUrl":"https://doi.org/10.5897/jpp2022.0620","url":null,"abstract":"Adansonia digitata is a tree plant used in west African local pharmacopoeia. This work aimed to evaluate the wound healing properties of its trunk bark’s hydroethanolic extract in wistar rats. The model of excision wound was used. Wistar rats were treated post-excision topically with neutral carbopol gel (Control), carbopol gels containing 2.5 and 5% of A. digitata hydroethanolic trunk bark extract (ADHE) and L-Mesitran. Pictures were taken post excision for analysis. Biopsies were realized for histological examination of the skin excised and for hydroxyproline determination. In vitro membrane stabilization anti-inflammatory test, DPPH and FRAP antioxidant tests, and phytochemical tests were performed. The results show a better rate of contraction in ADHE 2.5 and 5% animals (+56 ± 0.52% and +68 ± 0.24% respectively) as earlier as on day three post-excision. The hydroxyproline assay confirmed a better collagen production in ADHE 2.5% and 5% animals (+102.5 ± 2.9%, and +107 ± 3.0% respectively). Histological analysis showed a rapid re-epithelialization in ADHE animals. The anti-inflammatory test indicated an IC50 of 16.521 ± 0.525 µg/mL, very close to Aspirin (16.826 ± 0.341 µg/mL), showing a possible anti-inflammatory activity of ADHE. The extract reduced DPPH radical (IC50 of 344.985 ± 17.139 µg/mL) and ferric ions (EC50 = 178.689 ± 0.250 µg/mL). Phytochemical investigations revealed the presence of alkaloids, terpenoids, and polyphenolic compounds. Flavonoids in particular, with an amount of 51.694 ± 0.174 mgER/g, should explain the wound healing activity observed, making the hydroethanolic extract of A. digitata a potential remedy for excision wounds.","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87601507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jean Bangoua Mindiédiba, Hervé Coulidiati Tangbadioa, P. N. Aminata, Segda Abdoulaye, Amlabu Emmanuel, Anicet Ouedraogo Georges
Cancer is one of the major public health problems of the 21st century. Its incidence is increasing in both developed and low-income countries. This trend is justified by the change in people's lifestyles. Cancer of the prostate is one of major cancer types affecting male population. The present study focused on the use of methanolic extract of Striga hermonthica (Delile) Benth. to evaluate total phenolics and flavonoids contents, antioxidant activities, anti-proliferative and anti-migratory effects, using ATCC prostate cancer cell lines (22RV1, DU145, C4-2 and RWPE-1). Total phenolics and flavonoids content were estimated using spectrophotometric methods. Antioxidant potential was evaluated using three methods while cytotoxicity assay, anti-migratory assay and western blot analysis were used for the determination of extracts anti-proliferative activity on prostate cancer cells. Therefore, to assess apoptosis induction in prostate cancer cell lines, the effect of S. hermonthica (Delile) Benth. methanolic extract was investigated on procaspase-3, procaspase-8, and cyclin-B 2 expression. A range of half-dilution concentrations beginning from 500 µg/mL have been prepared to determine the IC 50 in the various tests. Antioxidant compounds were shown to be promising biomolecules, with particular interest in cancer management. Among plant derivative compounds, phenolics and derived molecules have endorsed antioxidant capacities and potential anti-proliferative activities. The best inhibition was obtained on C4-2 cells with an IC 50 = 65.76 µg/mL and a selectivity index of 2.35. The results obtained from this study could partially justify the traditional indications of this plant in the treatment of prostate cancer.
{"title":"Antitumoral effect of Striga hermonthica (Delile) Benth. methanolic extract is mediated by alterations on procaspase-3 and cyclin B expression in prostate cancer cell lines","authors":"Jean Bangoua Mindiédiba, Hervé Coulidiati Tangbadioa, P. N. Aminata, Segda Abdoulaye, Amlabu Emmanuel, Anicet Ouedraogo Georges","doi":"10.5897/jpp2022.0619","DOIUrl":"https://doi.org/10.5897/jpp2022.0619","url":null,"abstract":"Cancer is one of the major public health problems of the 21st century. Its incidence is increasing in both developed and low-income countries. This trend is justified by the change in people's lifestyles. Cancer of the prostate is one of major cancer types affecting male population. The present study focused on the use of methanolic extract of Striga hermonthica (Delile) Benth. to evaluate total phenolics and flavonoids contents, antioxidant activities, anti-proliferative and anti-migratory effects, using ATCC prostate cancer cell lines (22RV1, DU145, C4-2 and RWPE-1). Total phenolics and flavonoids content were estimated using spectrophotometric methods. Antioxidant potential was evaluated using three methods while cytotoxicity assay, anti-migratory assay and western blot analysis were used for the determination of extracts anti-proliferative activity on prostate cancer cells. Therefore, to assess apoptosis induction in prostate cancer cell lines, the effect of S. hermonthica (Delile) Benth. methanolic extract was investigated on procaspase-3, procaspase-8, and cyclin-B 2 expression. A range of half-dilution concentrations beginning from 500 µg/mL have been prepared to determine the IC 50 in the various tests. Antioxidant compounds were shown to be promising biomolecules, with particular interest in cancer management. Among plant derivative compounds, phenolics and derived molecules have endorsed antioxidant capacities and potential anti-proliferative activities. The best inhibition was obtained on C4-2 cells with an IC 50 = 65.76 µg/mL and a selectivity index of 2.35. The results obtained from this study could partially justify the traditional indications of this plant in the treatment of prostate cancer.","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88201773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Daher, R. Taleb, M. Mroueh, Bilal Nehmeh, Hachem Araji, Rawan H. Chakas, A. Daccache, Kamar H. Eid, Maria K. El Khoury, Pamela F. El Hayek, Hala T. Faddoul, Elio Junior Feghali, Ahmad Ghanem, Tony P. Haydar, Abir R. Jammoul, Sula H. Nasra, Nouhad Saade, Donna G. Tahan, J. Daher, Esther Tannoury, W. Shebaby
Laurus nobilis, commonly known as bay, is used in folk medicine as a remedy for many ailments. The present study investigates the protective effect of L. nobilis leaves extract against high fat diet-induced type 2 diabetes in rats. Animals were divided into group 1 (control), groups 2, 3, and 4 (bay leaves aqueous (AQ) extracts; 50, 100, and 250 mg/kg of body weight, respectively), and groups 5, 6, and 7 (bay leaves methanol/acetone (MeAc) extract; 50, 100, and 250 mg/kg of body weight, respectively). Animals were fed an isocaloric high fat diet for four weeks. The intake of bay leaves extracts was associated with a significant decreases in serum levels of glucose (AQ, 100 and 250 mg/kg; MeAc, 50, 100, and 250 mg/kg) and serum triglyceride (AQ, 250 mg/kg; MeAc, 100, and 250 mg/kg) as well as lower abdominal fat (all AQ and MeAc groups) and body weight gain (MeAc groups only). In conclusion, L. nobilis leaves extract intake provides a protective remedy against high fat diet-induced type 2 diabetes. Key words: Laurus nobilis, type 2 diabetes, bay leaves, high fat diet.
{"title":"Laurus nobilis leaves extract protects against high fat diet-induced type 2 Diabetes in rats","authors":"C. Daher, R. Taleb, M. Mroueh, Bilal Nehmeh, Hachem Araji, Rawan H. Chakas, A. Daccache, Kamar H. Eid, Maria K. El Khoury, Pamela F. El Hayek, Hala T. Faddoul, Elio Junior Feghali, Ahmad Ghanem, Tony P. Haydar, Abir R. Jammoul, Sula H. Nasra, Nouhad Saade, Donna G. Tahan, J. Daher, Esther Tannoury, W. Shebaby","doi":"10.5897/JPP2021.0609","DOIUrl":"https://doi.org/10.5897/JPP2021.0609","url":null,"abstract":"Laurus nobilis, commonly known as bay, is used in folk medicine as a remedy for many ailments. The present study investigates the protective effect of L. nobilis leaves extract against high fat diet-induced type 2 diabetes in rats. Animals were divided into group 1 (control), groups 2, 3, and 4 (bay leaves aqueous (AQ) extracts; 50, 100, and 250 mg/kg of body weight, respectively), and groups 5, 6, and 7 (bay leaves methanol/acetone (MeAc) extract; 50, 100, and 250 mg/kg of body weight, respectively). Animals were fed an isocaloric high fat diet for four weeks. The intake of bay leaves extracts was associated with a significant decreases in serum levels of glucose (AQ, 100 and 250 mg/kg; MeAc, 50, 100, and 250 mg/kg) and serum triglyceride (AQ, 250 mg/kg; MeAc, 100, and 250 mg/kg) as well as lower abdominal fat (all AQ and MeAc groups) and body weight gain (MeAc groups only). In conclusion, L. nobilis leaves extract intake provides a protective remedy against high fat diet-induced type 2 diabetes. \u0000 \u0000 Key words: Laurus nobilis, type 2 diabetes, bay leaves, high fat diet.","PeriodicalId":16801,"journal":{"name":"Journal of Pharmacognosy and Phytotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81136935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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