Hexane extract of Telosma cordata enhances neurite outgrowth via the epigenetically regulated genes expression in neuronal cells

IF 1.4 4区生物学 Q3 PLANT SCIENCES Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-11-15 DOI:10.15835/nbha51413204

Nguyet Thi DOAN, Hung Minh NGUYEN, Tuyet Loan Thi BUI, Huyen Trang Thi NGUYEN, Duong Hoang Nguyen, Truyen van Nguyen

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Abstract

Telosma cordata has long been consumed as foods and herbal medicine. The aim of this research is to investigate the potential biological activities of Telosma cordata flower (TCF) extracts and their molecular mechanisms in the treatment of neurological diseases, especially dementia. In general, the plant samples were successfully identified through DNA barcoding regions, utilizing matK, trnH-psbA, and rbcL markers. The ethanol extract and its fraction exhibited significantly high acetylcholinesterase inhibitory activity. Moreover, hexane extract of T. cordata displayed the most potent neurotrophic activity in a preliminary cell-based screening based on C6 cells neurite outgrowth. Additionally, hexane extract of T. cordata demonstrated the highest antioxidant activity. Finally, the hexane extract of T. cordata upregulated the expression of BDNF, NGF and acetyl H3 in C6 cells, affecting both mRNA and protein levels. These findings indicate that Telosma cordata could be a strong candidate for developing pharmacological drugs to treat dementia and neurodegenerative diseases.

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Telosma cordata 的己烷提取物可通过神经元细胞中的表观遗传调控基因表达增强神经元突起的生长

长期以来，人们一直将脐橙花作为食品和中药食用。本研究的目的是探讨虫草花（TCF）提取物在治疗神经系统疾病，尤其是痴呆症方面的潜在生物活性及其分子机制。总体而言，利用 matK、trnH-psbA 和 rbcL 标记，通过 DNA 条形码区域成功鉴定了植物样本。乙醇提取物及其馏分表现出极高的乙酰胆碱酯酶抑制活性。此外，在基于 C6 细胞神经突起生长的初步细胞筛选中，虫草的正己烷提取物显示出最强的神经营养活性。此外，T. cordata 的正己烷提取物显示出最高的抗氧化活性。最后，T. cordata 的正己烷提取物能上调 C6 细胞中 BDNF、NGF 和乙酰 H3 的表达，影响 mRNA 和蛋白质水平。这些研究结果表明，Telosma cordata可能是开发治疗痴呆症和神经退行性疾病的药理药物的有力候选者。

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来源期刊

Notulae Botanicae Horti Agrobotanici Cluj-napoca PLANT SCIENCES-

CiteScore

2.70

自引率

0.00%

发文量

118

审稿时长

3 months

期刊介绍： Notulae Botanicae Horti Agrobotanici Cluj-Napoca is a peer-reviewed biannual journal aimed at disseminating significant research and original papers, critical reviews and short reviews. The subjects refer on plant biodiversity, genetics and plant breeding, development of new methodologies that can be of interest to a wide audience of plant scientists in all areas of plant biology, agriculture, horticulture and forestry. The journal encourages authors to frame their research questions and discuss their results in terms of the major questions of plant sciences, thereby maximizing the impact and value of their research, and thus in favor of spreading their studies outcome. The papers must be of potential interest to a significant number of scientists and, if specific to a local situation, must be relevant to a wide body of knowledge in life sciences. Articles should make a significant contribution to the advancement of knowledge or toward a better understanding of existing biological and agricultural concepts. An international Editorial Board advises the journal. The total content of the journal may be used for educational, non-profit purposes without regard to copyright. The distribution of the material is encouraged with the condition that the authors and the source (Notulae Botanicae Horti Agrobotanici Cluj-Napoca or JCR abbrev. title Not Bot Horti Agrobo) are mentioned.