Yinghong Liu, Wenjuan Li, Chaohai Wang, Shuyun Chen, Gaiqing Wang
Background: Viral encephalitis (VE) is one of the common diseases of children with intracranial infection, it has come on urgent, progress is fast, and the clinical features of severe cases may even lead to disability, death, and other serious adverse prognostic outcomes, so seek in early diagnosis and prognosis of efficiency of the relevant indicators to stop in time and take effective means to prevent the further development is of great significance. Neopterin (NPT), as a factor that plays an important role in the process of validation development, has been relatively rarely studied in children with VE.
Methods: In this study, 127 cases of children with VE were retrieved from the TCGA database by bioinformatics, and their amplitude integrated electroencephalogram (AEEG) related information was collected at the same time. The neurodevelopmental status of VE children was evaluated according to the Gesell scale and divided into the good group (n=88) and the poor group (n=39). The differences in NPT expression and AEEG score between them were observed. In addition, the clinical data of 100 children without VE were screened from the database, and the differences in NPT expression and AEEG score between VE children and non-VE children were compared. The ROC curve was used to evaluate the clinical efficacy of NPT combined with AEEG in diagnosis and prognosis prediction. Kaplan-Meier was used to observe the effect of NPT high expression and low expression on poor prognosis of VE children.
背景：病毒性脑炎（VE）是儿童颅内感染的常见疾病之一，其来势急、进展快，临床特点是重症病例甚至可导致残疾、死亡等严重不良预后结局，因此寻求在早期诊断和预后有效率的相关指标以及时阻止并采取有效手段防止其进一步发展具有重要意义。蝶呤（NPT）作为一种在验证发展过程中起重要作用的因子，对VE患儿的研究相对较少：本研究通过生物信息学方法从 TCGA 数据库中检索了 127 例 VE 患儿，同时收集了他们的振幅综合脑电图（AEEG）相关信息。根据Gesell量表对VE患儿的神经发育状况进行评估，分为良好组（88例）和不良组（39例）。观察他们之间 NPT 表达和 AEEG 评分的差异。此外，还从数据库中筛选了 100 名无 VE 儿童的临床数据，并比较了 VE 儿童与非 VE 儿童在 NPT 表达和 AEEG 评分方面的差异。采用ROC曲线评估NPT结合AEEG在诊断和预后预测方面的临床疗效。采用 Kaplan-Meier 法观察 NPT 高表达和低表达对 VE 儿童不良预后的影响。
{"title":"The prognostic value of the NPT test combined with amplitude integrated electroencephalogram in children with VE and its bioreliability analysis.","authors":"Yinghong Liu, Wenjuan Li, Chaohai Wang, Shuyun Chen, Gaiqing Wang","doi":"10.5937/jomb0-43317","DOIUrl":"10.5937/jomb0-43317","url":null,"abstract":"<p><strong>Background: </strong>Viral encephalitis (VE) is one of the common diseases of children with intracranial infection, it has come on urgent, progress is fast, and the clinical features of severe cases may even lead to disability, death, and other serious adverse prognostic outcomes, so seek in early diagnosis and prognosis of efficiency of the relevant indicators to stop in time and take effective means to prevent the further development is of great significance. Neopterin (NPT), as a factor that plays an important role in the process of validation development, has been relatively rarely studied in children with VE.</p><p><strong>Methods: </strong>In this study, 127 cases of children with VE were retrieved from the TCGA database by bioinformatics, and their amplitude integrated electroencephalogram (AEEG) related information was collected at the same time. The neurodevelopmental status of VE children was evaluated according to the Gesell scale and divided into the good group (n=88) and the poor group (n=39). The differences in NPT expression and AEEG score between them were observed. In addition, the clinical data of 100 children without VE were screened from the database, and the differences in NPT expression and AEEG score between VE children and non-VE children were compared. The ROC curve was used to evaluate the clinical efficacy of NPT combined with AEEG in diagnosis and prognosis prediction. Kaplan-Meier was used to observe the effect of NPT high expression and low expression on poor prognosis of VE children.</p>","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"41 1","pages":"63-71"},"PeriodicalIF":2.5,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10943470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89675660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-31Epub Date: 2022-06-17DOI: 10.1080/15592324.2022.2086733
Dan Wang, Binyu Cui, Hanyu Guo, Yaxi Liu, Shuming Nie
C-repeat binding factor (CBF) subfamily genes encoding transcriptional activators are members of the AP2/ERF superfamily. CBFs play important roles in plant tolerance to abiotic stress. In this study, we identified and analyzed the structure, phylogeny, conserved motifs, and expression profiles of 12 CBFs of the grass species Lolium perenne cultured under abiotic stress. The identified LpCBFs were grouped into three phylogenetic clades according to their protein structures and motif organizations. LpCBF expression was differentially induced by cold, heat, water deficit, salinity, and abscisic acid, among which cold treatment induced LpCBF gene expression significantly. Furthermore, association network analysis indicated that different proteins, including certain stress-related proteins, potentially interact with LpCBFs. Altogether, these findings will enhance our understanding of LpCBFs protein structure and function in the regulation of L. perenne stress responses. Our results will provide valuable information for further functional research of LpCBF proteins in L. perenne stress resistance.
{"title":"Genome-wide identification and expression analysis of the CBF transcription factor family in <i>Lolium perenne</i> under abiotic stress.","authors":"Dan Wang, Binyu Cui, Hanyu Guo, Yaxi Liu, Shuming Nie","doi":"10.1080/15592324.2022.2086733","DOIUrl":"10.1080/15592324.2022.2086733","url":null,"abstract":"<p><p>C-repeat binding factor (CBF) subfamily genes encoding transcriptional activators are members of the AP2/ERF superfamily. CBFs play important roles in plant tolerance to abiotic stress. In this study, we identified and analyzed the structure, phylogeny, conserved motifs, and expression profiles of 12 CBFs of the grass species <i>Lolium perenne</i> cultured under abiotic stress. The identified LpCBFs were grouped into three phylogenetic clades according to their protein structures and motif organizations. <i>LpCBF</i> expression was differentially induced by cold, heat, water deficit, salinity, and abscisic acid, among which cold treatment induced <i>LpCBF</i> gene expression significantly. Furthermore, association network analysis indicated that different proteins, including certain stress-related proteins, potentially interact with LpCBFs. Altogether, these findings will enhance our understanding of LpCBFs protein structure and function in the regulation of <i>L. perenne</i> stress responses. Our results will provide valuable information for further functional research of LpCBF proteins in <i>L. perenne</i> stress resistance.</p>","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"44 1","pages":"2086733"},"PeriodicalIF":2.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89688205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The papers published in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Issue 4, Volume 51, 2023 represent new exciting research in different topics of life science, respectively in plant science, horticulture, agronomy, crop science, and forestry. Among the interesting articles we invite you to find news about: Role of silica nanoparticles in enhancing drought tolerance of cereal crops; The role of endophytes and rhizobacteria to combat drought stress in wheat; In vitro evaluation of the antiproliferative activity of Piper retrofractum on human cholangiocarcinoma cells; Hexane extract of Telosma cordata enhances neurite outgrowth via the epigenetically regulated genes expression in neuronal cells; Investigations on the chemical composition of volatile oils extracted from the leaves of spontaneous and cultivated Taxus baccata L. trees; The effect of arbuscular mycorrhizal inoculation and plant growth-promoting rhizobacteria on maize (Zea mays L.) under boron toxicity stress; Potential impact of iron oxide conjugated nano-fertilizer on growth, flowering and isozyme expression in Gardenia jasminoides; Water ecological requirements of Characeae taxa in eastern Spain; Agronomic biofortification with magnesium nanofertilizer and its effect on the nutritional quality of beans; ZnO nanoparticles improve bioactive compounds, enzymatic activity and zinc concentration in grapevine; Effect of mycorrhization on growth and physiology performance of Quercus species; Jointly soil properties affect N and P uptakes and utilizations in Pinus tabuliformis Carr. and Quercus liaotungensis Koidz. subjected to growing media with decomposed litter.
{"title":"Introduction pages, NBHA-CN 51(4), 2023","authors":"R. Sestras","doi":"10.15835/nbha51413576","DOIUrl":"https://doi.org/10.15835/nbha51413576","url":null,"abstract":"The papers published in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Issue 4, Volume 51, 2023 represent new exciting research in different topics of life science, respectively in plant science, horticulture, agronomy, crop science, and forestry. Among the interesting articles we invite you to find news about: Role of silica nanoparticles in enhancing drought tolerance of cereal crops; The role of endophytes and rhizobacteria to combat drought stress in wheat; In vitro evaluation of the antiproliferative activity of Piper retrofractum on human cholangiocarcinoma cells; Hexane extract of Telosma cordata enhances neurite outgrowth via the epigenetically regulated genes expression in neuronal cells; Investigations on the chemical composition of volatile oils extracted from the leaves of spontaneous and cultivated Taxus baccata L. trees; The effect of arbuscular mycorrhizal inoculation and plant growth-promoting rhizobacteria on maize (Zea mays L.) under boron toxicity stress; Potential impact of iron oxide conjugated nano-fertilizer on growth, flowering and isozyme expression in Gardenia jasminoides; Water ecological requirements of Characeae taxa in eastern Spain; Agronomic biofortification with magnesium nanofertilizer and its effect on the nutritional quality of beans; ZnO nanoparticles improve bioactive compounds, enzymatic activity and zinc concentration in grapevine; Effect of mycorrhization on growth and physiology performance of Quercus species; Jointly soil properties affect N and P uptakes and utilizations in Pinus tabuliformis Carr. and Quercus liaotungensis Koidz. subjected to growing media with decomposed litter.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"29 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138945930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Narges Abdar, Mehdi Zarei, A. Shahriari, Iman Mirmazloum
A greenhouse study was conducted to investigate the effect of arbuscular mycorrhiza fungus and plant growth-promoting rhizobacteria on maize (Zea mays L.) yield and boron (B) uptake under excessive B concentrations. Four levels of B (0, 10, 20 and 40 mg kg-1 soil) and four levels of microbial inoculation (non-inoculation, Rhizophagus intraradices, Micrococcus yunnanensis and co-inoculation) were applied. In general, elevated B decreased the maize relative yield, plant dry weight, plant height, total leaf area and chlorophyll content. The highest B uptake was recorded in the 20 mg kg-1 B treatments, whereas the lowest uptake was in B treatments of 0 and 40 mg B kg-1. Excessive B supply increased the proline content and electrolyte leakage of maize leaves. Microbial inoculation did not change the yield of B-stressed plants. In addition, mycorrhiza inoculation and co-inoculation increased the shoot and root B uptake. Maize plants growing at high B concentrations with or without microbial inoculation showed leaf edge burning and necrosis when compared with control treatments. Microbial inoculation improved the maize yield under B stress up to 20 mg B kg-1. Thus, inoculation with arbuscular mycorrhiza and plant growth promoting rhizobacteria can increase the maize tolerance under B toxicity. Nonetheless, the inoculation of bacterium and arbuscular mycorrhiza had a more alleviative effect when the plants were exposed to excess B.
{"title":"The effect of arbuscular mycorrhizal inoculation and plant growth-promoting rhizobacteria on maize (Zea mays L.) under boron toxicity stress","authors":"Narges Abdar, Mehdi Zarei, A. Shahriari, Iman Mirmazloum","doi":"10.15835/nbha51413473","DOIUrl":"https://doi.org/10.15835/nbha51413473","url":null,"abstract":"A greenhouse study was conducted to investigate the effect of arbuscular mycorrhiza fungus and plant growth-promoting rhizobacteria on maize (Zea mays L.) yield and boron (B) uptake under excessive B concentrations. Four levels of B (0, 10, 20 and 40 mg kg-1 soil) and four levels of microbial inoculation (non-inoculation, Rhizophagus intraradices, Micrococcus yunnanensis and co-inoculation) were applied. In general, elevated B decreased the maize relative yield, plant dry weight, plant height, total leaf area and chlorophyll content. The highest B uptake was recorded in the 20 mg kg-1 B treatments, whereas the lowest uptake was in B treatments of 0 and 40 mg B kg-1. Excessive B supply increased the proline content and electrolyte leakage of maize leaves. Microbial inoculation did not change the yield of B-stressed plants. In addition, mycorrhiza inoculation and co-inoculation increased the shoot and root B uptake. Maize plants growing at high B concentrations with or without microbial inoculation showed leaf edge burning and necrosis when compared with control treatments. Microbial inoculation improved the maize yield under B stress up to 20 mg B kg-1. Thus, inoculation with arbuscular mycorrhiza and plant growth promoting rhizobacteria can increase the maize tolerance under B toxicity. Nonetheless, the inoculation of bacterium and arbuscular mycorrhiza had a more alleviative effect when the plants were exposed to excess B.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"29 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138957323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. C. Dunăreanu, Gheorghița L. Piscanu, D. Bonea, Mihai Botu
Taking into account the rising demand for organic wheat and the need to guarantee both high productivity and quality of the crops, the choice of cultivar plays a crucial role. These intended objectives may solely be attained by employing the most appropriate cultivars for organic agriculture. The aim of this study was to evaluate the suitability of 15 wheat cultivars for cultivation in organic farming according to three important parameters for organic farmers and processors, namely grain yield, test weight and protein content. The field experiment took place over two consecutive growing seasons, in the center of the Oltenia region (southwest of Romania) under organic farming conditions. The results showed significant effects of the climatic conditions in the year of study, but also of the cultivar on all studied parameters. The effect of genotype-environment interactions was significant only for test weight and protein content. On average, over the two years of study, the grain yield was 3899 kg ha-1, the test weight was 70.8 kg hl-1, and the protein content was 10.1%. The recently released cultivar ‘Voinic’ obtained the maximum grain yield (4755 kg ha-1) and also a maximum test weight and protein content (73.9 kg hl-1, respectively 10.7%). In conclusion, it follows that by using cultivars suitable for organic farming, good and quality yields can be obtained that can be a suitable raw material for baking industry.
{"title":"Assessment of common wheat (Triticum aestivum L.) yield and quality under organic farming in the southwest of Romania","authors":"I. C. Dunăreanu, Gheorghița L. Piscanu, D. Bonea, Mihai Botu","doi":"10.15835/nbha51413113","DOIUrl":"https://doi.org/10.15835/nbha51413113","url":null,"abstract":"Taking into account the rising demand for organic wheat and the need to guarantee both high productivity and quality of the crops, the choice of cultivar plays a crucial role. These intended objectives may solely be attained by employing the most appropriate cultivars for organic agriculture. The aim of this study was to evaluate the suitability of 15 wheat cultivars for cultivation in organic farming according to three important parameters for organic farmers and processors, namely grain yield, test weight and protein content. The field experiment took place over two consecutive growing seasons, in the center of the Oltenia region (southwest of Romania) under organic farming conditions. The results showed significant effects of the climatic conditions in the year of study, but also of the cultivar on all studied parameters. The effect of genotype-environment interactions was significant only for test weight and protein content. On average, over the two years of study, the grain yield was 3899 kg ha-1, the test weight was 70.8 kg hl-1, and the protein content was 10.1%. The recently released cultivar ‘Voinic’ obtained the maximum grain yield (4755 kg ha-1) and also a maximum test weight and protein content (73.9 kg hl-1, respectively 10.7%). In conclusion, it follows that by using cultivars suitable for organic farming, good and quality yields can be obtained that can be a suitable raw material for baking industry.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"122 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sulaiman, Hossam S. EL-BELTAGI, W. F. Shehata, Aziz AHMAD, M. F. Hassim, Maha L. Hadid
Cereal crops are essential for providing essential nutrients and energy in the daily human diet. Additionally, they have a crucial role as a significant constituent of cattle feed, hence enhancing meat production. Drought, being an abiotic stressor, adversely affects the growth and yield of numerous crops on a global scale. This issue poses a significant and pressing obstacle to maintaining global cereal crop production and ensuring food security. Nanoparticles have become a valuable resource for improving cereal crop yield and productivity under ongoing rapid climate change and escalating drought conditions. Among these, silica nanoparticles (SiNPs) have demonstrated their potential for agricultural applications in regions with limited water availability. Drought stress has detrimental effects on cereal crops, impacting their growth, metabolic, and physiological processes, hampering water and nutrient absorption, disrupting cellular membranes, damaging the photosynthetic apparatus, and reducing antioxidant activities by altering gene expression. SiNPs help preserve cellular membranes, regulate water balance, and improve water and nutrient absorption, resulting in a substantial enhancement in plant growth under water-deficit conditions. SiNPs also protect the photosynthetic system and enhance its efficiency, facilitate the accumulation of phenolics, hormones, osmolytes, antioxidant activities, and gene expression, thus empowering plants with increased resistance to drought stress. Moreover, SiNPs decrease leaf water loss by promoting stomatal closure, primarily by fostering the accumulation of abscisic acid (ABA) and mitigating oxidative stress damage by activating the antioxidant defence system and reducing reactive oxygen species (ROS). However, a limited number of studies examine the role of SiNPs in cereal crops under drought stress conditions. In this review, we highlighted the promising potential of SiNPs to improve cereal crop resilience by changing morpho-histological traits, antioxidant properties, and gene expression to maintain food security in drought-prone areas. This study will aid researchers in using SiNPs as an environmentally benign way to improving drought resistance in cereal crops in order to fulfill global food supply needs.
{"title":"Role of silica nanoparticles in enhancing drought tolerance of cereal crops","authors":"Sulaiman, Hossam S. EL-BELTAGI, W. F. Shehata, Aziz AHMAD, M. F. Hassim, Maha L. Hadid","doi":"10.15835/nbha51413480","DOIUrl":"https://doi.org/10.15835/nbha51413480","url":null,"abstract":"Cereal crops are essential for providing essential nutrients and energy in the daily human diet. Additionally, they have a crucial role as a significant constituent of cattle feed, hence enhancing meat production. Drought, being an abiotic stressor, adversely affects the growth and yield of numerous crops on a global scale. This issue poses a significant and pressing obstacle to maintaining global cereal crop production and ensuring food security. Nanoparticles have become a valuable resource for improving cereal crop yield and productivity under ongoing rapid climate change and escalating drought conditions. Among these, silica nanoparticles (SiNPs) have demonstrated their potential for agricultural applications in regions with limited water availability. Drought stress has detrimental effects on cereal crops, impacting their growth, metabolic, and physiological processes, hampering water and nutrient absorption, disrupting cellular membranes, damaging the photosynthetic apparatus, and reducing antioxidant activities by altering gene expression. SiNPs help preserve cellular membranes, regulate water balance, and improve water and nutrient absorption, resulting in a substantial enhancement in plant growth under water-deficit conditions. SiNPs also protect the photosynthetic system and enhance its efficiency, facilitate the accumulation of phenolics, hormones, osmolytes, antioxidant activities, and gene expression, thus empowering plants with increased resistance to drought stress. Moreover, SiNPs decrease leaf water loss by promoting stomatal closure, primarily by fostering the accumulation of abscisic acid (ABA) and mitigating oxidative stress damage by activating the antioxidant defence system and reducing reactive oxygen species (ROS). However, a limited number of studies examine the role of SiNPs in cereal crops under drought stress conditions. In this review, we highlighted the promising potential of SiNPs to improve cereal crop resilience by changing morpho-histological traits, antioxidant properties, and gene expression to maintain food security in drought-prone areas. This study will aid researchers in using SiNPs as an environmentally benign way to improving drought resistance in cereal crops in order to fulfill global food supply needs.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"33 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138955859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plum pox virus (PPV) is considered the most detrimental viral pathogen of stone fruits, causing important economic losses. Exhaustive studies revealed that D, M and Rec are the prevalent strains of PPV in Europe. While different experimental reports revealed that PPV-M is more aggressive than PPV-D, limited information is available about the competitiveness of PPV-Rec under field conditions. Also, symptoms developed by PPV-Rec infection are scarcely documented. To increase this knowledge, we selected and monitored an experimental plum orchard already contaminated by both PPV-D and PPV-Rec. The disease spread was monitored by visual observation and ELISA testing each year along five consecutive vegetative periods. The dynamic of single and mixed infections was assessed by molecular strain typing (IC-RT-PCR) of all newly and single-infected trees. While the disease prevalence increased almost linearly, the number of single PPV infected trees (either by PPV-Rec or by PPV-D) remained relatively stable during the five-year period of monitoring, which is explainable by the continual increase of the number of trees infected with both strains together. No significant difference in the progression rate between D and Rec strains was found when considering their presence in both single and mixed infections. Although the types of PPV symptoms developed on leaves and fruits varied among cultivars, no clear-cut D or Rec strain-specific symptoms were developed within the same cultivar. Overall results revealed that PPV-D and PPV-Rec strains had similar behaviour in terms of competitiveness and symptoms developed under our experimental field conditions.
{"title":"Are D and Rec strains of Plum pox virus similar or different in terms of competitiveness and symptomatology?","authors":"I. Zagrai, L. Zagrai","doi":"10.15835/nbha51413526","DOIUrl":"https://doi.org/10.15835/nbha51413526","url":null,"abstract":"Plum pox virus (PPV) is considered the most detrimental viral pathogen of stone fruits, causing important economic losses. Exhaustive studies revealed that D, M and Rec are the prevalent strains of PPV in Europe. While different experimental reports revealed that PPV-M is more aggressive than PPV-D, limited information is available about the competitiveness of PPV-Rec under field conditions. Also, symptoms developed by PPV-Rec infection are scarcely documented. To increase this knowledge, we selected and monitored an experimental plum orchard already contaminated by both PPV-D and PPV-Rec. The disease spread was monitored by visual observation and ELISA testing each year along five consecutive vegetative periods. The dynamic of single and mixed infections was assessed by molecular strain typing (IC-RT-PCR) of all newly and single-infected trees. While the disease prevalence increased almost linearly, the number of single PPV infected trees (either by PPV-Rec or by PPV-D) remained relatively stable during the five-year period of monitoring, which is explainable by the continual increase of the number of trees infected with both strains together. No significant difference in the progression rate between D and Rec strains was found when considering their presence in both single and mixed infections. Although the types of PPV symptoms developed on leaves and fruits varied among cultivars, no clear-cut D or Rec strain-specific symptoms were developed within the same cultivar. Overall results revealed that PPV-D and PPV-Rec strains had similar behaviour in terms of competitiveness and symptoms developed under our experimental field conditions.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"113 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138959249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khaled M.A. Ramadan, Hossam S. EL-BELTAGI, Taia A. Abd El Mageed, Khaled E. Mazrou, G. Mohamed, M. El‐Saadony, F. El-Saadony, Mohamed H.H. Roby, H. Saudy, A. Abou-Sreea
Anise is one of the plants with therapeutic potential, which is classified among the most important medicinal plants with interesting biological effects. Its components could be perceived so as “natural” and “safe” alternatives to antibiotics as well as they are applied in different industries such as food and cosmetic purposes. Selenium (Se) is an essential micronutrient, however, its importance to improve oil yield and quality of anise has not been adequately investigated, specifically under drought. Therefore, two successive seasons were conducted to investigate the effect of selenium foliar application upon anise plants under drought stress. Selenium was applied at three different concentrations (0.0 1.0 and 2.0 mM denoted Se0, Se1 and Se2, respectively) along with two levels of crop evapotranspiration (ET): Full irrigation, 100% of ET (FI) and 60% of ET (DI). The promotive effect of combinations of DI × Se1 or Se2 (for Fv/Fm, RWC%, and MSI% in the first season) and DI × Se2 (for Fv/Fm, and MSI% in the second season) were as similar as FI × Se2. Compared to the counterpart control treatment (DI × Se0), the highest increases in total free amino acids (31.5 and 31.6%), total soluble sugars (84.2 and 86.4%) and free proline content (84.2 and 86.4%) were recorded with application of DI × Se2 practice in both seasons, respectively. Under DI, Se2 recorded the maximum values of root length, shoot fresh weight and shoot dry weight in the second season. Under drought, the increases in seed yield due to application of Se1 and Se2 amounted to 1.72 and 1.62 folds in the 1st season and 1.50 and 1.43 folds in 2nd one, respectively. The most effective practice for improving IWUE was Se1× DI in both seasons, followed by Se2 × DI. Based on the chromatographical detection, the maximum values of Anethole were recorded with FI × Se2 while L-Linalool has greatly increased with DI × Se2. In conclusion, the growers in arid and semi-arid zones are advised to use selenium (2.0 mM) in anise fertilization to mitigate the adverse impacts of drought, and keeping crop yield and quality.
{"title":"Significance of selenium in ameliorating the effects of irrigation deficit via improving photosynthesis efficiency, cell integrity, osmo-protectants, and oil profile of anise crop","authors":"Khaled M.A. Ramadan, Hossam S. EL-BELTAGI, Taia A. Abd El Mageed, Khaled E. Mazrou, G. Mohamed, M. El‐Saadony, F. El-Saadony, Mohamed H.H. Roby, H. Saudy, A. Abou-Sreea","doi":"10.15835/nbha51413437","DOIUrl":"https://doi.org/10.15835/nbha51413437","url":null,"abstract":"Anise is one of the plants with therapeutic potential, which is classified among the most important medicinal plants with interesting biological effects. Its components could be perceived so as “natural” and “safe” alternatives to antibiotics as well as they are applied in different industries such as food and cosmetic purposes. Selenium (Se) is an essential micronutrient, however, its importance to improve oil yield and quality of anise has not been adequately investigated, specifically under drought. Therefore, two successive seasons were conducted to investigate the effect of selenium foliar application upon anise plants under drought stress. Selenium was applied at three different concentrations (0.0 1.0 and 2.0 mM denoted Se0, Se1 and Se2, respectively) along with two levels of crop evapotranspiration (ET): Full irrigation, 100% of ET (FI) and 60% of ET (DI). The promotive effect of combinations of DI × Se1 or Se2 (for Fv/Fm, RWC%, and MSI% in the first season) and DI × Se2 (for Fv/Fm, and MSI% in the second season) were as similar as FI × Se2. Compared to the counterpart control treatment (DI × Se0), the highest increases in total free amino acids (31.5 and 31.6%), total soluble sugars (84.2 and 86.4%) and free proline content (84.2 and 86.4%) were recorded with application of DI × Se2 practice in both seasons, respectively. Under DI, Se2 recorded the maximum values of root length, shoot fresh weight and shoot dry weight in the second season. Under drought, the increases in seed yield due to application of Se1 and Se2 amounted to 1.72 and 1.62 folds in the 1st season and 1.50 and 1.43 folds in 2nd one, respectively. The most effective practice for improving IWUE was Se1× DI in both seasons, followed by Se2 × DI. Based on the chromatographical detection, the maximum values of Anethole were recorded with FI × Se2 while L-Linalool has greatly increased with DI × Se2. In conclusion, the growers in arid and semi-arid zones are advised to use selenium (2.0 mM) in anise fertilization to mitigate the adverse impacts of drought, and keeping crop yield and quality.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":" 42","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138962563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Din, C. Mihăescu, Diana I. POPESCU (STEGARUS), D. Vîlcoci, Georgiana Cîrstea, Ionela Sărdărescu, Diana-Elena Vizitiu, Alina Paunescu, Ion Mitrea, R. Mitrea
The pathogens Alternaria solani and Fusarium oxysporum f. sp. lycopersici are of significant interest from a pathogenic perspective in the context of tomato cultivation. This study focuses on evaluating the fungicidal and fungistatic effects of different synthetic substances and natural compounds on the development of these two investigated pathogens. The fungicidal agents employed comprised fosetyl aluminum at a concentration of 0.3%, azoxystrobin at 0.2%, and metiram at 0.3%, while the natural extracts investigated included those derived from European birthwort, celandine and sage, each tested at concentrations of 0.5%, 2%, 9%, and 15%. The assessment of mycelial growth inhibition was conducted utilizing Vincent’s formula. Additionally, the total polyphenol content (TPC) within the extracts was determined via the Folin-Ciocalteu spectrophotometric method in accordance with Frum et al. (2022). Furthermore, the antioxidant capacity of the extracts was evaluated using the DPPH radical scavenging method (2,2-diphenyl-1-picrylhydrazyl) and high-performance liquid chromatography (HPLC). Our research findings yielded noteworthy results, specifically, extracts derived from sage and celandine, particularly when present at a concentration of 15%, exhibited a fungistatic effect. This effect was particularly remarkable when compared to the performance of the synthetic fungicide azoxystrobin at a concentration of 0.2% when challenged with the Alternaria pathogen. These results suggest the potential utility of sage and celandine extracts as eco-friendly alternatives for mitigating fungal pathogens in tomato crops, warranting further investigation and consideration within agricultural practices.
{"title":"Ecological control of mycotic pathogens in tomato crops - alternatives to synthetic pesticides","authors":"A. Din, C. Mihăescu, Diana I. POPESCU (STEGARUS), D. Vîlcoci, Georgiana Cîrstea, Ionela Sărdărescu, Diana-Elena Vizitiu, Alina Paunescu, Ion Mitrea, R. Mitrea","doi":"10.15835/nbha51413492","DOIUrl":"https://doi.org/10.15835/nbha51413492","url":null,"abstract":"The pathogens Alternaria solani and Fusarium oxysporum f. sp. lycopersici are of significant interest from a pathogenic perspective in the context of tomato cultivation. This study focuses on evaluating the fungicidal and fungistatic effects of different synthetic substances and natural compounds on the development of these two investigated pathogens. The fungicidal agents employed comprised fosetyl aluminum at a concentration of 0.3%, azoxystrobin at 0.2%, and metiram at 0.3%, while the natural extracts investigated included those derived from European birthwort, celandine and sage, each tested at concentrations of 0.5%, 2%, 9%, and 15%. The assessment of mycelial growth inhibition was conducted utilizing Vincent’s formula. Additionally, the total polyphenol content (TPC) within the extracts was determined via the Folin-Ciocalteu spectrophotometric method in accordance with Frum et al. (2022). Furthermore, the antioxidant capacity of the extracts was evaluated using the DPPH radical scavenging method (2,2-diphenyl-1-picrylhydrazyl) and high-performance liquid chromatography (HPLC). Our research findings yielded noteworthy results, specifically, extracts derived from sage and celandine, particularly when present at a concentration of 15%, exhibited a fungistatic effect. This effect was particularly remarkable when compared to the performance of the synthetic fungicide azoxystrobin at a concentration of 0.2% when challenged with the Alternaria pathogen. These results suggest the potential utility of sage and celandine extracts as eco-friendly alternatives for mitigating fungal pathogens in tomato crops, warranting further investigation and consideration within agricultural practices.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":" 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138961648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This review examines the possible clinical uses of extracts from mushrooms with medicinal properties, particularly their role as gastroprotective agents. After conducting a thorough examination of the literature, we identified and chose publications that presented six distinct types of mushroom extracts. These extracts have been experimentally demonstrated to have gastroprotective effects in animal models. Animals like rats or mice were used as subjects in experimental models to generate stomach ulcers through various approaches, such as chemical induction or inducing stress. Studies have shown that mushroom extracts are efficacious in facilitating the healing process of stomach ulcers. Several fundamental modes of action include anti-inflammatory and antioxidant effects, along with enhanced preservation of stomach mucus. No clinical trials pertaining to this topic have been uncovered. However, the initial positive outcomes gained serve as a basis for additional investigation on this subject.
{"title":"The role of medicinal mushrooms as gastroprotective agents: A short narrative review","authors":"Aida Puia, Diana Schlanger, I. C. Puia","doi":"10.15835/nbha51413532","DOIUrl":"https://doi.org/10.15835/nbha51413532","url":null,"abstract":"This review examines the possible clinical uses of extracts from mushrooms with medicinal properties, particularly their role as gastroprotective agents. After conducting a thorough examination of the literature, we identified and chose publications that presented six distinct types of mushroom extracts. These extracts have been experimentally demonstrated to have gastroprotective effects in animal models. Animals like rats or mice were used as subjects in experimental models to generate stomach ulcers through various approaches, such as chemical induction or inducing stress. Studies have shown that mushroom extracts are efficacious in facilitating the healing process of stomach ulcers. Several fundamental modes of action include anti-inflammatory and antioxidant effects, along with enhanced preservation of stomach mucus. No clinical trials pertaining to this topic have been uncovered. However, the initial positive outcomes gained serve as a basis for additional investigation on this subject.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":" 98","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138961084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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