Bacteriological Quality of Fresh Cow Milk From Dairy Farms in Parts of Kaduna State, Nigeria

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Abstract

The study was carried out to determine the bacteriological quality of farm fresh raw cow milk with emphasis on the detection of Staphylococcus species. A total of 592 quarter milk samples, 30 bulk milk samples and 27 swab samples of the hands of milk men were examined from 12 dairy farms in Kaduna and Zaria. The bacteriological quality of the milk samples were determined by both the California Mastitis Test and the Total Viable Staphylococcal Count. The prevalence of subclinical mastitis from positive California Mastitis Test (³+) was 24.5%. The mean Staphylococcal count was 4.2 log10 cfu/ml. The number of suspected Staphylococcal isolates that were Gram positive and catalase positive were 103, which were then biochemically screened down clearly to 51, with their identities confirmed using the Microbat Microgen Kit. Among the Staphylococcal species, Staphylococcus aureus showed the highest population of phenotypic identity with 38%. This organism is important from public health point of view as they have been associated with the onset of food poisoning in human beings.
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尼日利亚卡杜纳州部分地区奶牛场鲜牛奶的细菌学质量
这项研究旨在确定农场新鲜生牛奶的细菌学质量,重点是检测葡萄球菌的种类。共检测了来自卡杜纳和扎里亚 12 个奶牛场的 592 份四分之一牛奶样本、30 份散装牛奶样本和 27 份挤奶工手拭样本。牛奶样本的细菌学质量是通过加利福尼亚乳腺炎测试和总存活葡萄球菌计数确定的。加利福尼亚乳腺炎测试(³+)呈阳性的亚临床乳腺炎发病率为 24.5%。平均葡萄球菌计数为 4.2 log10 cfu/ml。革兰氏阳性和过氧化氢酶阳性的疑似葡萄球菌分离物为 103 个,经生化筛选后明确为 51 个,并使用 Microbat Microgen Kit 确认了它们的身份。在葡萄球菌中,金黄色葡萄球菌的表型一致性最高,达到 38%。从公共卫生的角度来看,这种微生物非常重要,因为它们与人类食物中毒的发生有关。
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